Callus culture in vitro in the experimental evaluation of drought resistance in cereals (review)

Круглова, Н.Н.; Seldimirova, O.A.; Zinatullina, А.Е.

doi:10.33952/2542-0720-2021-1-25-124-139

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…В последние годы исследователи обращают самое пристальное внимание на такое направление диагностической лабораторной оценки стресс-устойчивости злаковых растений, как использование селективных методов культуры in vitro клеток, тканей и органов [2,3,[24][25][26][27][28]. Особенно обширные исследования посвящены анализу в селективных экспериментальных условиях культуры in vitro засухоустойчивости различных видов хлебных злаков [29][30][31][32].…”

Section: Introductionunclassified

“…Использование методов культуры in vitro в качестве экспериментальных способов оценки стресс-устойчивости эксплантов и далее регенерантов имеет ряд преимуществ и недостатков. Подробно эти вопросы рассмотрены во многих обзорных работах [3,26,28,33,34]. В контексте темы данного обзора подчеркнем то несомненное преимущество, что параметры условий культуры in vitro, заданные аналогично экстремальным условиям in vivo, дают возможность детально анализировать реакции эксплантов/регенерантов на действие конкретных абиотических стрессов (в ряде случаев -нескольких стресс-факторов в комплексе), что сложно изучить в тепличных и полевых условиях из-за изменчивого характера действия этих стрессов.…”

Section: Introductionunclassified

“…Непрямой путь образования регенерантов хлебных злаков и связанное с этим изучение различных вопросов индукции формирования морфогенных каллусов и их развития на индукционной и регенерационной средах in vitro -предмет давних научных интересов авторов данной обзорной статьи. Анализ результатов многолетних экспериментальных исследований позволил авторам предложить оценивать морфогенные каллусы in vitro как модельные системы при изучении сложнейшей проблемы морфогенеза растений [42][43][44] и при биотехнологических исследованиях устойчивости растений к абиотическим стресс-факторам и действия обработок антистрессовыми препаратами [26,28,45,46]. В последнее десятилетие авторы данной статьи в своих исследованиях обращают большое внимание на изучение прямого пути получения регенерантов хлебных злаков в условиях эмбриокультуры in vitro -как в теоретическом плане [47], так и в разработке прикладных способов использования культивируемых зародышей в селективной оценке засухоустойчивости.…”

Section: Introductionunclassified

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Embryo culture in vitro in the experimental evaluation of drought resistance in cereals (review)

Круглова¹,

Seldimirova²,

Zinatullina³

2021

TVAN

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Drought is an unfavorable combination of meteorological conditions when plants experience a long-term water deficiency both in the air and soil. This is one of the most common abiotic stressors, which leads not only to significant crop losses but also rises threat to food security. Researchers are actively developing ways to breed drought-tolerant cultivars of economically valuable crops, especially cereals – the main food resource. One of the promising areas of biotechnological evaluation of the resistance of existing and newly created cereal genotypes to drought for breeding purposes is the use of culture in vitro. In this case, embryos at the particular stage of development are used as explants (so-called embryo culture in vitro). The review aims to analyze the literature and own data on the production of cereal regenerants in embryo culture in vitro under selective experimental conditions of imitation of physiological drought. It has been shown that in vitro cultivation of immature embryos at a critical stage of relative autonomy is especially promising. This kind of embryo does not depend on the physiological factors of the maternal organism and can autonomously give rise to the fully developed plant under adequate conditions in vitro and later ex vitro. This allows the biotechnologist to obtain regenerants directly, excluding an additional time-consuming stage of the formation of morphogenic calli in vitro. As follows, the time required for expensive experiments is also reduced. Data on the identification of the critical stage of the relative autonomy of the cereal embryogenesis are presented. Criterion (proposed by the authors) for identifying this stage by the ability of the embryos to complete embryogenesis and form the seedlings on a hormone-free medium in vitro and give rise to the full developed regenerants ex vitro has been analyzed. Furthermore, the analysis of the laboratory germination of the obtained caryopses was carried out. It was discovered that in spring soft wheat, for example, such stage, corresponding to the formation of all organs in the embryo, occurs 15 days after pollination. The issues of using relatively autonomous embryos in the biotechnological assessment of the genotype drought resistance under selective conditions in vitro are considered.

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Section: Introductionunclassified

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Embryo culture in vitro in the experimental evaluation of drought resistance in cereals (review)

Круглова¹,

Seldimirova²,

Zinatullina³

2021

TVAN

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In Vitro Culture of Autonomous Embryos as a Model System for the Study of Plant Stress Tolerance to Abiotic Factors (on the Example of Cereals)

Круглова

Zinatullina

2022

Biol Bull Rev

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Development of regenerated plants of interspecific wheat hybrids using immature embryos as explants

Kalinina,

Dontsova,

Chertkova

et al. 2023

Zernovoe hozâjstvo Rossii

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Nowadays particular attention in winter wheat breeding is paid to the introduction of biotechnological methods. The culture of immature embryos in vitro is an alternative way to produce hybrids that often do not develop fully in vivo. The purpose of the current study was to estimate the ability of immature embryos of interspecific winter wheat hybrids for callus induction, morphogenesis, and plant regeneration on artificial nutrient media, as well as to select optimal conditions for obtaining full-fledged plants with all the botanical characteristics of the species. The research material included 5 hybrid combinations of interspecific crosses between durum (Triticum durum DESF.) and common (Triticum aestivum L.) winter wheat. The material was collected during one field season (2022). Immature embryos were used as explants. Culture media were according to Murashige-Skoog (MS), and they differed in the content of hormones and organic substances. There has been found that the hybrid combinations were characterized by a variety of morphogenic responses. The manifestation of callusogenesis was not high enough and averaged 22–32 % depending on the nutrient medium. Rhizogenesis averaged from 1–6.7 %, and ended with the formation of roots. The process of formation of embryoid- and hemmorizogenesis was best demonstrated by the hybrid combination No.1, and on two media. All processes of morphogenesis excepting ‘rhizogenesis’ varied greatly among hybrid combinations. The largest number of regenerated plants was obtained from hybrid combination No.1 (61 pcs., 54.5 %). After vernalization and acclimatization, 38 % of the plants survived from the number of planted embryos. The largest number of heads (79 pcs.) was formed according to hybrid combination No.1, on average 1.46 heads per plant. However, not all heads were fertile. The results of the correlation analysis made it possible to identify indicators that closely correlated with the amount of plant regeneration, namely, the high yield of embryoid- and hemmorizogenic structures ensured a high frequency of plant regeneration (r = 0.769).

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Callus culture in vitro in the experimental evaluation of drought resistance in cereals (review)

Cited by 3 publications

References 19 publications

Embryo culture in vitro in the experimental evaluation of drought resistance in cereals (review)

Embryo culture in vitro in the experimental evaluation of drought resistance in cereals (review)

In Vitro Culture of Autonomous Embryos as a Model System for the Study of Plant Stress Tolerance to Abiotic Factors (on the Example of Cereals)

Development of regenerated plants of interspecific wheat hybrids using immature embryos as explants

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