Callus formation in albino Wistar rats after femur fracture assessed by visible spectroscopy

Orban, Emese; Z, Pap; Micu, Andreea Maria; Şipoş, Remus; Fechete, Radu

doi:10.1016/j.bbrc.2022.09.114

Cited by 5 publications

(3 citation statements)

References 25 publications

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“…On the other hand, in the ovarectomized batch treated with feno brate, it could be observed that the cytoarchitectonics of the bone tissue is similar in the structure of the molecular exchanges with the control batch, especially with the non-ovarectomized control batch. This distinct effect on cytoarchitectonic change, if correlated with evidence from studies using visible spectroscopy, demonstrated that in ovarectomized rats feno brate treatment promotes the callus process, whereas simvastatin delays this process [31], could explain why primary callus remodeling is slower in ovarectomized and simvastatin-treated rats.…”

Section: Discussionsupporting

confidence: 56%

Assessment of bone tissue cytoarchitectonics by 2D 1 H NMR relaxometry maps

Orban,

Pap,

Sipos

et al. 2024

Preprint

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Introduction. Bone is a complex tissue that fulfils the role of a resistance structure. This quality is most commonly assessed by bone densitometry, but bone strength may not only be related to bone mineral density but also to the preservation of bone cytoarchitectonics. Material and methods. The study included two groups of rats, ovarectomized and non-ovarectomized. Each group was divided into three batches: control, simvastatin-treated and fenofibrate-treated. In the ovarectomized group, hypolipidemic treatment was instituted at 12 weeks post ovarectomy. One rat from each of the 6 batches was sacrificed 8 weeks after the start of treatment in the group. The experimental study was performed using a Bruker Minispec mq 20 spectrometer operating at a frequency of 20 MHz, subsequently also performed by 1H T2-T2 molecular exchange maps. Results. The results were represented by T2-T2 molecular exchange maps that showed, comparatively, both pore size and their interconnectivity at the level of the posterior femoral epiphysis, being able to evaluate both the effect of estrogen on bone tissue biology and the effect of the lipid-lowering medication simvastatin and fenofibrate both in the presence and absence of estrogen. Conclusion. T 2-T2 molecular exchange maps showed that the absence of estrogen results in an increase in bone tissue pore size and interconnectivity. In the presence of estrogen, lipid-lowering medication, both simvastatin and fenofibrate alter bone tissue cytoarchitectonics by reducing pore interconnectivity. In the absence of estrogen, fenofibrate improves bone tissue cytoarchitectonics, the T2-T2 molecular exchange map being similar to that of non-osteoporotic bone tissue.

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Section: Discussionsupporting

confidence: 56%

Assessment of bone tissue cytoarchitectonics by 2D 1 H NMR relaxometry maps

Orban,

Pap,

Sipos

et al. 2024

Preprint

Self Cite

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“…It was found that in non-ovariectomized rats, hypolipidemic medication with simvastatin and fenofibrate delayed the callus formation process, while with fenofibrate, this process was delayed much more. On the other hand, in the ovariectomized group of rats, the effects of the lipid-lowering drugs are opposite, so fenofibrate favors the callus formation process and simvastatin delays it [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Influence of Hypolipidemic Medication on Albino Wistar Rats’ Bone Tissue by NMR Diffusiometry

Orban,

Pap,

Fechete

et al. 2024

Medicina

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Introduction: The ongoing concern of the medical profession regarding chronic medication is related to increasing patient adherence and compliance to treatment and reducing medication side effects. In this respect, drugs represented by fixed-dose combinations of active substances within the same tablet have emerged. Such a principle can be extrapolated by following the potential beneficial effects that a chronic medication can have on chronic pathologies affecting different systems. Materials and Methods: The study included 48 female Albino Wistar rats, aged 16–18 months, which were divided into two groups: ovariectomized and non-ovariectomized rats. One batch of 12 non-ovariectomized rats received no treatment, becoming a control batch (NOVX-M). The ovariectomized (OVX) group was divided into 3 batches of 12 rats each: no treatment, control (OVX-M), fenofibrate-treated (OVX-F) and statin-treated (OVX-S) rats. At 12 weeks after ovariectomy, a femoral fracture occurred in the right hind limb of all animals included in the experiment To reveal the changes, at intervals of 2, 4, 6 and 8 weeks post-fracture, the proximal part of the femur was evaluated by NMR diffusiometry, which allows random motion of proton molecules expressed by self-diffusion coefficients, D, thus allowing analysis of the size and complexity of microscopic order cavities within biological structures, such as pores inside bones. Results: The effects of hypolipidemic medication in the absence of estrogen were evidenced, proving the beneficial effect that fenofibrate can have in preserving healthy tissue exposed to osteoporotic risk during the menopausal period. The effects of lipid-lowering medication are also influenced by the duration of administration. Conclusions: Osteoporosis and heart disease are two chronic pathologies that affect mainly female population in the second half of life, and proving the dual therapeutic potential of lipid-lowering medication may also have positive effects by increasing adherence and compliance to treatment.

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“…On the other hand, experimental testing in animal models has been used for the study of the early period of fracture healing [12][13][14], but these models can hardly be applied to humans, due to the differences at the anatomical level and between in vivo and in vitro behaviour.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Computational Model Simulating the Initial Callus Growth during Fracture Healing in Long Bones: Application to Different Fracture Types

et al. 2023

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Bone fractures are among the most common and potentially serious injuries to the skeleton, femoral shaft fractures being especially severe. Thanks to recent advances in the area of in silico analysis, several approximations of the bone healing process have been achieved. In this context, the objective of this work was to simulate the initial phase of callus formation in long bones, without a pre-meshed domain in the 3D space. A finite element approach was computationally implemented to obtain the values of the cell concentrations along the whole domain and evaluate the areas where the biological quantities reached the thresholds necessary to trigger callus growth. A voxel model was used to obtain the 3D domain of the bone fragments and callus. A mesh growth algorithm controlled the addition of new elements to the domain at each step of the iterative procedure until complete callus formation. The implemented approach is able to reproduce the generation of the primary callus, which corresponds to the initial phase of fracture healing, independently of the fracture type and complexity, even in the case of several bone fragments. The proposed approach can be applied to the most complex bone fractures such as oblique, severely comminuted or spiral-type fractures, whose simulation remains hardly possible by means of the different existing approaches available to date.

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Callus formation in albino Wistar rats after femur fracture assessed by visible spectroscopy

Cited by 5 publications

References 25 publications

Assessment of bone tissue cytoarchitectonics by 2D 1 H NMR relaxometry maps

Assessment of bone tissue cytoarchitectonics by 2D 1 H NMR relaxometry maps

Evaluation of the Influence of Hypolipidemic Medication on Albino Wistar Rats’ Bone Tissue by NMR Diffusiometry

Three-Dimensional Computational Model Simulating the Initial Callus Growth during Fracture Healing in Long Bones: Application to Different Fracture Types

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