2020
DOI: 10.1371/journal.pone.0220084
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Callus growth kinetics and accumulation of secondary metabolites of Bletilla striata Rchb.f. using a callus suspension culture

Abstract: Bletilla striata is an endangered traditional Chinese medicinal plant with multiple uses and a slow regeneration rate of its germplasm resources. To evaluate the callus growth kinetics and accumulation of secondary metabolites (SMs), a callus suspension culture was proven to be a valuable approach for acquiring high yields of medicinal compounds. An effective callus suspension culture for obtaining B. striata callus growth and its SMs was achieved with the in vitro induction of calluses from B. striata seeds. … Show more

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Cited by 41 publications
(30 citation statements)
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“…The synthesis of secondary metabolites in plants is closely related to the growth stage and environmental conditions, and each type of secondary metabolites has different biosynthetic pathways at different stages of growth. In general, the secondary metabolite concentration peaked during the exponential growth stage and decreased afterward during the stationary stage of the callus culture [38]. The NPs can elicit oxidative damage in plants by generating reactive oxygen species (ROS), such as H 2 O 2 and hydroxyl radical, causing damage to lipid membranes, proteins, and nucleic acids, which results in denaturation of cellular components or activation of the defense system in plants [39,40].…”
Section: Discussionmentioning
confidence: 99%
“…The synthesis of secondary metabolites in plants is closely related to the growth stage and environmental conditions, and each type of secondary metabolites has different biosynthetic pathways at different stages of growth. In general, the secondary metabolite concentration peaked during the exponential growth stage and decreased afterward during the stationary stage of the callus culture [38]. The NPs can elicit oxidative damage in plants by generating reactive oxygen species (ROS), such as H 2 O 2 and hydroxyl radical, causing damage to lipid membranes, proteins, and nucleic acids, which results in denaturation of cellular components or activation of the defense system in plants [39,40].…”
Section: Discussionmentioning
confidence: 99%
“…This technology is one of the most important methods for the biosynthesis of various secondary metabolites from the callus. In various medicinal plants, the callus suspension culture system has been recognized throughout the world but the wounded L. radiata callus and analysis of secondary metabolites have not yet been studied [ 14 , 15 , 16 ]. Previously, few studies have been carried out to analyze the secondary metabolite content in the bulb, leaf, and root of L. radiata [ 4 ].…”
Section: Introductionmentioning
confidence: 99%
“…The culture system was improved based on our former study (Pan et al, 2020). The loose and bright yellow callus obtained after two subculture processes for about 30 days were used as explants for suspension culturing and inoculating into 100 mL culture flask which containing 35 mL liquid medium.…”
Section: Suspension Culture Systemmentioning
confidence: 99%