Callus in<i>Rosmarinus officinalis</i>L. (Lamiaceae): A morphoanatomical, histochemical and volatile analysis

Boix, Yilan Fung; Arruda, Rosani do Carmo de Oliveira; Defaveri, Anna Carina Antunes e; Sato, Alice; Lage, Celso Luiz Salgueiro; ́rio, C. P. Victo

doi:10.1080/11263504.2012.751067

Cited by 14 publications

(10 citation statements)

References 17 publications

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“…These results are similar to that reported about leaf explants dedifferentiation of rosemary after 9 days of culture [10][11][12]16]. Leelavathi and Narendra [14], obtained rosemary callus, with BAP (8.88 µM), and total differentiation at 28 days of culture, and Boix et al [7], reported callus formation with 2,4-D (0.86, 1.7, and 2.6 µM) on MS medium [13], similar to this study.…”

Section: Discussionsupporting

confidence: 89%

“…Leaves were washed in a NaOCl (3%) solution for 10 min; explants were transferred to ethanol (70%) for 30 s and rinsed with sterile distilled water three times under laminar flow chamber. Leaves were cut into 0.3 mm sections and placed on Petri dishes containing 20 mL of MS medium [13] supplemented with 4 g/L Phytagel R , 30 g/L sucrose, pH 5.8, growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D) (2.6 µM) and 6-benzylaminopurine (8.8 µM) for callus initiation [7,14]. Explants were incubated at 16 h light/8 h dark and 25 ± 2°C.…”

Section: In Vitro Culture and Callus Inductionmentioning

confidence: 99%

“…In vitro systems are useful experimental tools in order to manipulate dedifferentiation process and secondary metabolites production [7]. Friable calli is used to generate plant cell suspension cultures, which are largely used as a source of large-scale production of secondary metabolites for therapeutic use [8].…”

Section: Introductionmentioning

confidence: 99%

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Chemical characterization of leaves and calli extracts of Rosmarinus officinalis by UHPLC‐MS

et al. 2019

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Rosmarinus officinalis L. (Lamiaceae) is an aromatic plant widely popular mainly due to its uses in traditional medicine as an anti-inflammatory, diuretic and antimicrobial, as well as in the prevention and treatment of diseases. These biological activities are mainly related to the presence of phenolic and terpenic compounds. This work reports a chemical profile analysis of extracts from leaves and calli of rosemary obtained by both pressurized liquid extraction and maceration. Chemical profiles were determined on calli extracts of 3, 6, 9, and 15 days of culture; chemical characterization and quantification of compounds was carried out using ultrahigh performance liquid chromatography-mass spectrometry. A total of 53 metabolites were identified in callus and 47 compounds in leaf extracts, of which 25 correspond to phenolic compounds, mainly flavonoids and flavones, 13 terpenes that include phenolic terpenes and one diterpenolactone, two glycosides which correspond to 6-O-caffeoyl-␤-D-fructofuranosil-(2→1)-␣-D-glucopyranoside and primulaverin, an aromatic compound identified as fenantrenone and a growth regulator 12-hydroxy jasmonic acid. These results showed that undifferentiated rosemary cells accumulate the same compounds identified mainly in highly specialized tissues such as leaves. The plant cell culture supply the possibility of developing biotechnological processes to obtain compounds of commercial interest.

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Section: Discussionsupporting

confidence: 89%

Section: In Vitro Culture and Callus Inductionmentioning

confidence: 99%

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Chemical characterization of leaves and calli extracts of Rosmarinus officinalis by UHPLC‐MS

et al. 2019

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“…Further major components were 1.8-cineole, camphor and verbenone. Similar essential oil composition was reported earlier in rosemary leaves (Tucker and Maciarello, 1986;Boix et al, 2011;Affholder et al, 2013;Sagawa et al, 2013;Loussouarn et al, 2017), peltate glandular hairs (Sagawa et al, 2013) or even callus (Boix et al, 2013). However, essential oil composition strongly varies among cultivars (Tucker and Maciarello, 1986) and developmental stages or even during seasons (Lakusic and Slavkovska, 2013).…”

Section: The Effect Of Prolonged Darkness On the Essential Oil Compossupporting

confidence: 83%

The Effect of Light on Plastid Differentiation, Chlorophyll Biosynthesis, and Essential Oil Composition in Rosemary (Rosmarinus officinalis) Leaves and Cotyledons

et al. 2020

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It is unclear whether light affects the structure and activity of exogenous secretory tissues like glandular hairs. Therefore, transmission electron microscopy was first used to study plastid differentiation in glandular hairs and leaves of light-grown rosemary (Rosmarinus officinalis "Arp") plants kept for 2 weeks under ambient light conditions. During our detailed analyses, among others, we found leucoplasts with tubuloreticular membrane structures resembling prolamellar bodies in stalk cell plastids of peltate glandular hairs. To study the effect of darkness on plastid differentiation, we then dark-forced adult, light-grown rosemary plants for 2 weeks and observed occasionally the development of new shoots with elongated internodes and pale leaves on them. Absorption and fluorescence spectroscopic analyses of the chlorophyllous pigment contents, the native arrangement of the pigment-protein complexes and photosynthetic activity confirmed that the first and second pairs of leaf primordia of darkforced shoots were partially etiolated (contained low amounts of protochlorophyll/ide and residual chlorophylls, had etio-chloroplasts with prolamellar bodies and low grana, and impaired photosynthesis). Darkness did not influence plastid structure in fifth leaves or secretory tissues (except for head cells of peltate glandular hairs in which rarely tubuloreticular membranes appeared). The mesophyll cells of cotyledons of 2week-old dark-germinated rosemary seedlings contained etioplasts with highly regular prolamellar bodies similar to those in mesophyll etio-chloroplasts of leaves and clearly differing from tubuloreticular membranes of secretory cells. Analyses of the essential oil composition obtained after solid phase microextraction and gas chromatography-mass spectroscopy showed that in addition to light, the age of the studied organ (i.e., first leaf primordia and leaf tip vs. fifth, fully developed green leaves) and the type of the organ

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“…Currently, it is contributing enormously to food production, crop improvement, production of secondary metabolites and conservation of endangered species (Boix et al 2012;Minutolo et al 2012). However, one of the major problems of in vitro plant cultivation is the high level of somaclonal variation during long cultivation periods.…”

Section: Introductionmentioning

confidence: 99%

Effects of long-term culture ofAstragalus chrysochloruscallus onmorphology, genetic structure, gene expression and metabolism

Turgut-Kara

Kahraman

2013

Plant Biosystems - An International Journal Dealing with all As

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To cite this article: N. Turgut-Kara & B. Ü. Kahraman (2015) Effects of long-term culture of Astragalus chrysochlorus callus onmorphology, genetic structure, gene expression and metabolism, AbstractThe objective of this study was to investigate the phenotypic and genetic effects of long-term (12 years) in vitro culturing of Astragalus chrysochlorus callus tissue. Murashige and Skoog medium supplemented with 0.5 mg/l 2,4-dichlorophenoxyacetic acid was used for the production of young callus (2 months old) from hypocotyl explants. Morphological analysis showed that long-term cultures were well adapted, with greener and more friable calli, and a higher growth index (2.11^0.39) was compared with that in short-term cultures (1.2^0.73). Genetic variation between the two types of calli was investigated by randomly amplified polymorphic DNA analysis, which showed different amplification patterns with a polymorphism level of about 45%. We furthermore measured RNA transcript levels for two key enzymes of the important stress-related phenylpropanoid pathway, PAL and C4H, via quantitative polymerase chain reaction. The gene expression levels of C4H and PAL were 0.205-and 0.584-fold lower in old cultures than in young cultures. Metabolic analysis by high performance thin layer chromatography clearly showed differences in flavonoid type metabolic content. Together, these data show that long-term culturing of callus tissue leads to genetic heterogeneity and variations in secondary metabolite content.

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Callus inRosmarinus officinalisL. (Lamiaceae): A morphoanatomical, histochemical and volatile analysis

Cited by 14 publications

References 17 publications

Chemical characterization of leaves and calli extracts of Rosmarinus officinalis by UHPLC‐MS

Chemical characterization of leaves and calli extracts of Rosmarinus officinalis by UHPLC‐MS

The Effect of Light on Plastid Differentiation, Chlorophyll Biosynthesis, and Essential Oil Composition in Rosemary (Rosmarinus officinalis) Leaves and Cotyledons

Effects of long-term culture ofAstragalus chrysochloruscallus onmorphology, genetic structure, gene expression and metabolism

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