Callus induction and secondary metabolite profile from <i>Elephantopus scaber</i> L.

Abstract

Elephantopus scaber L. is a plant that has potential as traditional medicine. Callus induction and production of secondary metabolite content can be increased by culture callus using plant growth regulators. This study was purposed to investigate the effect of IBA and kinetin concentration on the induction and secondary metabolite profile of callus from E. scaber L. leaves. Leaves explant of E. scaber L. were cultured on MS medium with various combination concentrations of IBA and kinetin for 6 weeks and then callus was extracted using methanol. Secondary metabolite content from the resulting extract was analyzed using the phytochemical screening method. The result showed that the treatment of IBA 2.0 mg/L and kinetin 1.0 mg/L and treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L are the fastest combination concentration to induce callus at 5.33 ± 0.577 days. Treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L produced callus with the highest fresh weight and dry weight at 0.7016 ± 0.0588 grams and 0.0766 ± 0.0062 grams, respectively. The morphology of calluses grown during this study was compact with various colors appearance, such as light green, brownish green, and brown. Secondary metabolite content of methanol extract of callus E. scaber L. are flavonoids, alkaloids, terpenoids, and saponins.

Callus Induction and Bioactive Compounds Production from Various Cultivars of Medicago sativa L. (alfalfa)

“…In our cases, total tannin content was at different levels in all tested genotypes under callus culture including 1 mg /L 2,4-D and 0.0125 mg /L kinetin. These findings are consistent with one of the first reports on callus induction and secondary metabolite profile obtained from Elephantopus scaber (Junairiah et al, 2021). A similar result of callus induction and production of phenolic compounds production under callus cultures of Nepeta binaloudensis Jamzad (Lamiaceae) was also reported (Sagharyan et al, 2020).…”

“…Plant growth regulators are largely used to stimulate callus, as they boost cell growth by inducing cell division and elongation through synergistic, antagonistic, and additive interactions. 2,4-dichlorophenoxyacetic acid (2,4-D, an auxin) has been commonly used either alone or combined with cytokinins, specially 6-benzylaminopurine (BAP), to induce callus initiation and determine bioactive molecules in tissue culture (Junairiah et al, 2021). However, the dosage and combination of these plant growth regulators must be identified for each species (Loredo-Carrillo et al, 2013).…”

Callus induction of Calotropis gigantea using BAP and 2,4-D in vitro

“…Because of the difference in fluid content, turgor pressure develops, allowing osmosis to bring nutrients and water from the medium into the cells. Cell walls would become more rigid as a result, resulting in a compact callus [18].…”

Keanekaragaman Tumbuhan Bawah Di Taman Wisata Alam (Twa) Gunung Baung Pasuruan Jawa Timur

“…Spesies Elephantopus scaber ini memiliki kategori intensitas cahaya matahari dan kondisi lingkungan yang sangat tinggi [23]. Elephantopus scaber ini termasuk tanaman yang akan tumbuh dan berkembang dengan optimal bila kondisi tanah tempat hidupnya sesuai dengan kebutuhan nutrisi terhadap unsur hara [34] Selanjutnya hasil koefisien korelasi spesies Commelina erecta dengan parameter intensitas cahaya, kadar air tanah dan bahan organik tanah memperoleh korelasi positif dengan nilai parameter intensitas cahaya -1,00 nilai korelasi parameter kadar air tanah -1,00 dan nilai parameter bahan organik tanah -1,00. Nilai korelasi tergolong negatif menunjukan bahwa arah hubungan berbanding terbalik antara variabel yang satu dengan lainnya.…”