Callus induction and somatic embryogenesis in Stevia rebaudiana Bertoni as a medicinal plant

Keshvari, Tahereh; Najaphy, Abdollah; Kahrizi, Danial; Zebarjadi, Alireza

doi:10.14715/cmb/2018.64.2.9

Cited by 30 publications

(12 citation statements)

References 10 publications

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“…In our study, the calli started to appear 10 days after the induction treatment, with varying sizes, color, consistency and morphogenesis. The results observed in this study corroborate the findings for Stevia rebaudiana Bertoni, where the time of callus induction was six days using 1 mg L -1 BAP + 1 mg L -1 NAA for both media (MS and B5) and explant types (leaf and internode) (Keshvari et al, 2018). On the other hand, other authors have reported no significant difference between interaction effects of explant type × hormone concentration on callus induction percentage in Cuminum cyminum L. (Kazemi; and Mentha pulegium L. (Jafari;.…”

Section: Embryogenic Callus Induction and Multiplication Of Leaf And supporting

confidence: 89%

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In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

Rodrigues

Almeida²,

Lêdo

et al. 2020

Ciênc. agrotec.

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Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as somatic embryogenesis, can be used as an alternative method for rapid and large-scale plant regeneration. The objectives of this study were to induce callogenesis in Vernonia condensata from different types of explants and to evaluate the structural aspects of the development of pro-embryogenic masses of this species by means of histological analyses. The formation of calli was induced from leaf explants and internodal segments, which were inoculated in EME medium supplemented with 50 g L-1 sucrose, 0.5 g L-1 malt extract and 2.68 μM NAA, plus varying concentrations of BAP (0.00, 2.22, 4.44 or 8.88 μM). After 40 days, the following morphogenetic traits were evaluated: intensity of callus formation, intensity of oxidation, callus texture, and morphogenesis. The calli with embryogenic masses were analyzed by light and scanning electron microscopy. Both types of explants were responsive regarding callogenesis, with the BAP concentration of 4.44 μM promoting the formation of friable calli associated with a larger percentage of calli with embryogenic masses. Cells from leaf explants and internodal segments were able to dedifferentiate and change into embryonic structures.

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Section: Embryogenic Callus Induction and Multiplication Of Leaf And supporting

confidence: 89%

“…Observations from other authors indicate that the type of medium and explant are important factors in quantity and quality of calli obtained from explants (Keshvari et al, 2018). Kumlaya and Ercisli, (2015) also noted that the callus induction percentage and friability of the produced calli varied widely between explants.…”

Section: Callus Oxidationmentioning

confidence: 94%

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

Rodrigues

Almeida²,

Lêdo

et al. 2020

Ciênc. agrotec.

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“…Phytohormones are another key influencing factors for SE. Most plant species undergoing SE are dependent on the powerful action of auxin and cytokinin, especially 2,4dichlorophenoxyacetic acid (2,4-D) which regulates and balances endogenous auxin levels (Illyas Ahmad et al, 2013;Mahendran and Bai, 2016;Keshvari et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Direct and Indirect Somatic Embryogenesis Induction in Camellia oleifera Abel

et al. 2021

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Camellia oleifera Abel. is an important woody oil species; however, the shortage of rapid and industrialized seedling culture is a large constraint on the development of the tea oil industry. Somatic embryogenesis (SE) is one of the main powerful biotechnological tools for plant mass regeneration, but the largely unknown SE in C. oleifera limits the scale production of clonal plants. In this study, we described a high-efficiency SE system via direct and indirect pathways in C. oleifera and investigated the effect of genotype, explant age and phytohormones on SE. In the direct pathway, somatic embryos were highly induced from immature seeds 220 days after full blossom, and the development of embryoids was achieved with a combination of 0.19 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.05 mg/L thidiazuron (TDZ). In the indirect pathway, embryogenic calli were induced from the same explants in medium containing 1.5 mg/L 2,4-D, while 0.75 mg/L 2,4-D treatment led to high proliferation rates for embryogenic calli. The addition of 0.19 mg/L 2,4-D alone stimulated the production of globular embryos while causing a 75% loss of the induction rate in the heart embryo stage. Upon transfer of the globular embryos to phytohormone-free medium, an optimal induction rate of 62.37% from globular embryos to cotyledonary embryos was obtained. These data suggest that the subsequent differentiation process after the globular embryo stage in ISE is more similar to an endogenous phytohormones-driven process. Mature embryos germinated to produce intact plantlets on half-strength MS basal medium with a regeneration rate of 63.67%. Histological analysis confirmed the vascular bundle isolation of embryoids from the mother tissue. We further studied the different varieties and found that there were no significant genotype differences for SE induction efficiency in C. oleifera. Thus, we established a high-efficiency induction system for direct and indirect somatic embryogenesis (ISE) in C. oleifera and regenerated intact plantlets via SE, not organogenesis. ISE has a more complicated induction and regulatory mechanism than direct somatic embryogenesis. The improved protocol of SE would benefit mass propagation and genetic manipulation in C. oleifera.

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“…Currently, in vitro culture of callus has been reported as an alternative method for the production of biomass aiming at the production of secondary metabolites, especially for the ease of induction and proliferation, being a simpler process than organogenic or embryogenic responses, which allows increasing the in vitro plant biomass, one of the main economic limitations in the production of secondary metabolites in vitro [ 8 ]. In addition, the induction and proliferation of calli in vitro allow good repeatability and standardization of responses [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Calli Production Resulted in Different Profiles of Plant-Derived Medicinal Compounds in Phyllanthus amarus

2020

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The efficient production of plant-derived medicinal compounds (PDMCs) from in vitro plants requires improvements in knowledge about control of plant or organ development and factors affecting the biosynthesis pathway of specific PDMCs under in vitro conditions, leading to a realistic large-scale tool for in vitro secondary metabolite production. Thus, this study aimed to develop an in vitro technique, through the induction and proliferation of calli, for production of plant fresh weight, and to compare the PDMC profile obtained from the plants versus in vitro calli of Phyllanthus amarus. It was successfully possible to obtain and proliferate two types of calli, one with a beige color and a friable appearance, obtained in the dark using Murashige and Skoog (MS) medium plus 2,4-dichlorophenoxyacetic acid (2,4-D), and a second type with a green color, rigid consistency, and nonfriable appearance obtained under light conditions and MS medium plus 6-benzyladenine (6-BA). In vitro micropropagated plants that gave rise to calli were also acclimatized in a greenhouse and cultivated until obtaining the mass for PDMC analysis and used as a control. While the micropropagated-derived plants concentrated the lignans niranthin, nirtetralin, and phyllanthin, the Phyllanthus amarus calli proliferated in vitro concentrated a completely different biochemical profile and synthesis of compounds, such as betulone, squalene, stigmasterol, and β-sitosterol, in addition to others not identified by GC-MS database. These results demonstrate the possibility of applying the calli in vitro from Phyllanthus amarus for production of important PDMCs unlike those obtained in cultures of differentiated tissues from field plants.

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Callus induction and somatic embryogenesis in Stevia rebaudiana Bertoni as a medicinal plant

Cited by 30 publications

References 10 publications

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

Direct and Indirect Somatic Embryogenesis Induction in Camellia oleifera Abel

In Vitro Calli Production Resulted in Different Profiles of Plant-Derived Medicinal Compounds in Phyllanthus amarus

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