Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Marisa, Fajri; Hidayati, Lisna; Sasongko, Aries Bagus; Nuringtyas, Tri Rini

doi:10.29303/jbt.v21i2.2629

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…Callus induction with 2,4-D 1 ppm; 1.5 ppm; 2 ppm; BAP 0.5 ppm + 2,4-D 2 ppm; and BAP 1 ppm + 2,4-D 1 ppm had a friable texture. The callus's loosely arranged cells and high division frequency contribute to its friability [17]. Based on observation, high concentrations of 2,4-D stimulated callus formation with a friable texture.…”

Section: Callus Morphologymentioning

confidence: 99%

Callus induction of Calotropis gigantea using BAP and 2,4-D in vitro

Muthiah

Sakya

Setyawati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Calotropis gigantea is a plant not widely used but has potential as a medicinal plant. The phytochemical compound can be produced faster using a tissue culture and a combination of growth regulators. The research objectives were to study the effect and determine the best concentration of BAP and 2,4-D in callus induction of Calotropis gigantea. This research was carried out in the Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sebelas Maret University, from September to November 2021. A month-old Calotropis gigantea stem was used as an explant. The research used three replicates of a completely randomized design with 25 combination treatments. The culture medium was MS with BAP (0; 0.5; 1; 1.5; 2 ppm) and 2,4-D (0; 0.5; 1; 1.5; 2 ppm) supplements. Parameters observed in this research were callus formation time, callus colour, and callus texture. The results showed that a combination of BAP 0.5 ppm + 2,4-D 1.5 ppm could induce callus faster than other treatments, 6 days. Callus morphology with green color and compact texture resulted from a combination of BAP 1.5 ppm + 2,4-D 1.5 ppm treatment.

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Section: Callus Morphologymentioning

confidence: 99%

Callus induction of Calotropis gigantea using BAP and 2,4-D in vitro

Muthiah

Sakya

Setyawati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…multiply, and then continue to grow(Idris and Paserang, 2019). According toMarisa et al (2021), physiologically fresh weight Callus…”

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confidence: 99%

Callus Induction In Leucaena (Leucaena leucocephala (Lam.) de Wit) As An Effort To Provide Target Transformation Through Agrobacterium tumefaciens

Arieswari,

Astarini,

Hardini

et al. 2022

Int. J. Biosci. Biotech.

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Leucaena is a plant that produces biomass productivity in the form of hardwood for fuel with low humidity and high calorific value. However, Leucaena is also classified as an invasive plant which can cause the urgency of native plant species and ecosystems in Indonesia. Therefore, the formation of sterile Leucaena needs to be done, one of which is through genetic transformation using Agrobacterium tumefaciens. Callus is used as a target for transformants in the genetic transformation process, so it is necessary to use appropriate media and PGR. This study aimed to determine the type of media and the concentration of 2,4-D on callus induction. This research is an experimental study with a completely randomized design (CRD) method with two factors. The first factor is the type of media (MS and WPM) and the second factor is the concentration of 2,4-D (0; 0.25; 0.50; 0.75; 1.00; 1.25 and 1.50 mgL-1 ). Each treatment was repeated three times so that 42 experimental units were obtained. Parameters observed were callus initiation, callus fresh weight (gram), callus texture and color. Quantitative data is analyzed by analysis of variance (ANOVA). The results showed that the use of media had a significant effect (P<0.05) on callus fresh weight. The use of 2,4- D concentration had a significant effect (P>0.05) on callus texture. The use of WPM media resulted in the fastest callus emergence time (6.67±0.57), the best callus texture (crumb callus type 2) and the best callus color (green). Meanwhile, the highest fresh weight (2,48±0.83) was in the use of MS media. The fastest callus emergence time occurred in the control (without the addition of 2,4-D) (7.33±0.57 and 6.67±0.57), the highest average fresh callus weight (2,48±0.83 and 2.35±0.32) occurred in the treatment with the addition of 1.00 mgL-1 2,4-D with a crumb callus texture of type 2 and callus green color only appeared in the treatment with a concentration of 0.25 mgL-1.

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Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Cited by 2 publications

References 12 publications

Callus induction of Calotropis gigantea using BAP and 2,4-D in vitro

Callus induction of Calotropis gigantea using BAP and 2,4-D in vitro

Callus Induction In Leucaena (Leucaena leucocephala (Lam.) de Wit) As An Effort To Provide Target Transformation Through Agrobacterium tumefaciens

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