Callus induction via different growth regulators from cotyledon explants of indigenous chick pea (Cicer arietinum L.) cultivars KK-1 and Hassan-2K

Khan, Saleem; Ahmad, Farhad; Ali, Farhad; Khan, Hakim Ali; Khan, Abdul Ghafoor; Swati, Zahoor Ahmad

doi:10.5897/ajb10.2378

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…These two factors have a direct influence on the callogenesis rate and are decisive for the optimization of the callogenic potential of Cicer arietinum (Shahab-ud-din et al, 2011). Notably, the combination of 2 mg of IAA with 2 mg of kinetin induced a rather interesting callogenesis rate, of about 80%, considering the recalcitrant nature of the tissues of Cicer arietinum (Sani and Mustapha, 2010).This rate was recorded in all the genotypes, as well as with the different explants that were tested, which contradicts the results of Khan et al (2011), who reported that the response of two native chickpea genotypes, namely 'KK1' and 'Hassan-2 K', subjected to in vitro culture conditions identical to those of the present study, expressed different callogenic abilities.…”

Section: Discussionmentioning

confidence: 76%

Callogenesis in Cicer arietinum and identification of a genotype resistant to Ascochyta rabiei

2020

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The chickpea (Cicer arietinum) is one of the leguminous species most appreciated by consumers in the Mediterranean basin, while being an important source of protein. Nevertheless, its crop yields are greatly limited by several biotic and abiotic stresses, the main one being Ascochyta rabiei, the causal agent of anthracnose. As traditional breeding methods have proved to be ineffective in controlling this pathogen, resorting to biotechnological methods is necessary. Therefore, in this study, the callogenic capacity of stem and leaflet explants from three genotypes of chickpea, namely ‘FLIP 84-92 C’, ‘ILC 32-97’, and ‘ILC 263’, cultured on Murashige and Skoog (MS) medium with different hormonal balances of auxins (indole-3-acetic acid [IAA] and 2,4-dichlorophenoxyacetic acid [2,4-D]) and cytokinin (kinetin), was determined. For all the genotypes, high percentages of callogenesis were recorded in the different explants grown on an MS medium with 2 mg of both IAA and kinetin. Then, a patho-system of Cicer arietinum calluses with Ascochyta rabiei was investigated, followed by a histological assessment of this interaction. The presence of the fruiting bodies of the pathogen was revealed in the calluses of the ‘ILC 32-97’ and ‘ILC 263’ genotypes. Notably, the latter showed a high sensitivity to the pathogen, as indicated by an abundance of pycnidia in its tissues. As for the ‘FLIP 84-92 C’ genotype, the histological sections showed a total absence of inter- and intracellular fruiting bodies of the pathogen in the callus tissues. Therefore, this genotype was considered as resistant to Ascochyta rabiei.

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Section: Discussionmentioning

confidence: 76%

Callogenesis in Cicer arietinum and identification of a genotype resistant to Ascochyta rabiei

2020

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“…Hormonal concentration 2.0 mg/l 2, 4-D provided the best result for callus induction, and medium supplemented with 0.1 mg/l BAP and 1.0 mg/l 2, 4-D require fewer times (4.25 days) to initiate callus as well as a medium containing 1.0 mg/l 2, 4-D gave highest growth rate of callus of chickpea (Chenar et al 2015). For chickpea cultivar KK-1, the highest callus frequency (97%) was observed on MS medium supplemented with 4.0 mg/l 2, 4-D and 5.0 μM BAP after 28 days of culture and in chickpea cultivar Hassan-2K, the maximum 96% callus were recorded on MS medium with 4.0 mg/l 2, 4-D and 0.5 mg/l NAA after 28 days of culture (Saleem et al 2011). Compared with the previous studies on callus induction of chickpea revealed that the present investigation differs from them in terms of growth regulators used and their response to callus induction.…”

Section: Resultsmentioning

confidence: 93%

In vitro Regeneration of Chickpea (Cicer arietinum L.) cultivated in Bangladesh

Sarkar¹,

Sarkar²,

Akter³

et al. 2022

Plant Tissue Cult. & Biotech.

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An efficient, and reproducible in vitro regeneration protocol was developed for three varieties of chickpea (Cicer arietinum L.) named Barichhola-9, Barichhola-10, and Barichhola-11 using embryos with decapitated root tips as explants. The best response towards callus induction was obtained on MS medium supplemented with a combination of 2.0 mg/l BAP and 0.3 mg/l NAA. MS medium containing 3.0 mg/l BAP alone provided the highest number of shoots from the induced callus. MS medium with 0.5 mg/l NAA was found to be the best for root induction from in vitro regenerated shoots in all three varieties of chickpea. In vitro regenerated well-rooted plantlets were transplanted into a small pot containing a mixture of soil and cocodust for acclimatization. Regeneration responses were found to be identical for the three varieties of chickpea used in this study. The results of the present investigation will facilitate the in vitro regeneration of chicpea varieties as well as further genetic improvement of this economically valuable crop species through genetic transformation. Plant Tissue Cult. & Biotech. 32(2): 205-216, 2022 (December)

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“…Combination of 2,4-D and kinetin has been employed in the ratio of 1:5 and 1:10 in the concentration range from 1-25 µM. Among various range of PGRs supplemented, MS medium supplemented with 5µM 2,4-D and 10µM Kinetin have induced callus at the maximum level (90.5%) in case of cotyledon explants excised from three varieties of soybean viz CO1, CO2, and CO3.Various types of explants and different plant growth regulators concentrations were already tested in legume plants species like Vigna radiata (L.) (Rao et al, 2005), Vigna mungo (L.) (Mony et al, 2007), Phaseolus vulgaris (L.) (Mahamune et al, 2011) and Cicer arietinum (L.) (Khan et al, 2011). The limitations in developing callus from oilseed plants such as recalcitrant in nature has also been experienced in our attempts.…”

Section: Discussionmentioning

confidence: 99%

Combined Supplementation of 2, 4-D and Kinetin Elevates Callus Induction From Cotyledon Segments of Different Soybean Cultivars [Glycine Max (L.) Merril]

Muthu,

Shanmugasundaram,

Karuppan

et al. 2023

PLANT ARCHIVES

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Callus induction and embryogenesis were tested in Soybean (Glycine max (L.) Merril) using cotyledon segments of different cultivars. In this study, embryogenic calli have been produced from cotyledon segments of various soybean cultivars like CO1, CO2, and CO3. To assess the callus induction and embryogenic potential of cotyledon explants, Murashige and Skoog media were added with different combinations of auxin and cytokinin. Among the treatments, medium added through 2,4-D (5µM) + Kinetin (10µM) exhibited a good size of profuse greenish callus (90.5±0.1g) with highest fresh weight of 2.5±0.1g and higher proliferation of embryogenic calli from cotyledons of CO3 variety. Pretreatment of cotyledonary explants with 0.5% polyvinypyrollidone overcome the limitations in generating callus from cotyledon segment cut ends. The present study implies the use of combined supplementation of 2,4-D (5µM) + Kinetin (10µM) on callus induction and the possibility of micro propagation in soybean cultivars using the cotyledonary explants that will be useful for genetic improvement studies in soybean cultivars .

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Callus induction via different growth regulators from cotyledon explants of indigenous chick pea (Cicer arietinum L.) cultivars KK-1 and Hassan-2K

Cited by 5 publications

References 10 publications

Callogenesis in Cicer arietinum and identification of a genotype resistant to Ascochyta rabiei

Callogenesis in Cicer arietinum and identification of a genotype resistant to Ascochyta rabiei

In vitro Regeneration of Chickpea (Cicer arietinum L.) cultivated in Bangladesh

Combined Supplementation of 2, 4-D and Kinetin Elevates Callus Induction From Cotyledon Segments of Different Soybean Cultivars [Glycine Max (L.) Merril]

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