Callus proliferation of <i>Dendrobium</i> in liquid culture system using airlift bioreactor

Rachmawati, Fitri; Thamrin, M.; Soehendi, Rudy; Winarto, Budi

doi:10.17660/actahortic.2022.1334.30

Cited by 1 publication

(4 citation statements)

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“…D. Indonesia Raya Ina had the best response to initiation and proliferation of EC compared to D. Sonia Earsakul and D. Gradita 10 with 89.1% to 99.7% regeneration of explants and 87.5% to 98.7% formation of EC (Rachmawati et al 2016b). Additionally, D. CF22-58 produced a higher EC proliferation improvement up to 356.7% with a 4.6 multiplication rate than D. Dian Agrihorti (Rachmawati et al, 2022). Thus, D. Jayakarta had a better embryogenesis response than D. Zahra FR 62 and D. Gradita 31 (Winarto, 2012).…”

Section: Discussion Discussion Discussionmentioning

confidence: 88%

“…In this study, the most significant contributor to EC malformations was EC browning, accounted for 48.50% of cases in BIOS-1 (an airlift bioreactor with 10 g 150 mL -1 explant density and 5 vvm aeration rate). Callus browning occurs due to stressing of callus tissue friction and callus deposition, causing an imbalance between the aeration rate and the callus density used (Rachmawati et al, 2016(Rachmawati et al, , 2022. The friction between callus causes the rupture of vacuoles and stimulates the production of phenol compounds.…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

“…Orbital shaker was used as culture system for D. Gradita 31, and D. Indonesia Raya (Rachmawati et al, 2015). The combination of shoot tips explant, half strength MS medium, and airlift bioreactor were applied for D. Zahra FR 62, D. Gradita 10, D. Indonesia Raya Ina, and D. Balithi CF22-58 Rachmawati et al, 2014Rachmawati et al, , 2016aRachmawati et al, , 2019Rachmawati et al, , 2022. While, the combination of basal part of plantlets as an explant, half strength MS medium, and airlift bioreactor for D. lasianthera x D. antennatum (Sasmita et al, 2022).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

“…Several critical aspects existed in establishing a mass propagation protocol for Dendrobium via somatic embryogenesis in the initiation stage. Optimal EC initiation using a basal part explant was determined in a half-strength MS medium containing 1.5 mg L -1 TDZ and 0.5 mg L -1 BAP (Rachmawati et al, 2019(Rachmawati et al, , 2022. Overcoming browning incidence while maintaining and improving EC growth under a liquid culture system is a crucial issue tackled during the proliferation stage.…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

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Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

RACHMAWATI,

PRAMANIK,

SHINTIAVIRA

et al. 2024

Not Bot Horti Agrobo

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The study focuses on two Indonesia Dendrobium varieties, D. Dian Agrihorti (DDA) and D. Syifa Agrihorti (DSA), which have potential to be commercialized, but face limitations on the availability of qualified seedlings sustainably. The research aimed to establish an in vitro propagation protocol using a Temporary Immersion System (TIS) to produce high-quality seedlings efficiently. Various factors, including varieties, media, plant growth regulators, anti-phenol compounds, organic additives, and TIS settings, were investigated using the basal part of the plantlet as the explant source. Key findings revealed that DDA outperformed DSA across all observed variables. In the initiation phase, basal plantlets cultured in Murashige and Skoog (MS) medium supplemented with 1.0 mg L-1 thidiazuron and 0.5 mg L-1 N-6 benzylaminopurine enhanced embryogenic callus (EC) formation, with a 13.5-day initiation period, 72% potential explant growth, 0.41 cm callus size, and a 3.45 rate of multiplication. During the proliferation stage, the addition of 150 mg L-1 ascorbic acid (AC) and the application of a TIS with a 30-minute dry period and a 15-minute wet period resulted in a 515.5% increase in EC fresh weight for DDA accompanied by a 6.16 multiplication rate. Regeneration of shoots was achieved using a Vacin and Went medium with 150 g L-1 banana extract, yielding 29.2 shoots per clump. Subsequent rooting of the shoots in 2 g L-1 Hyponex® medium with 20 g L-1 sugar and 2% AC proved successful. Acclimatization of plantlets with Cycas rumpii bulk demonstrated a 100% survivability rate. The established propagation protocol for DDA holds significant potential for application to other Dendrobium varieties, offering a sustainable and efficient method for meeting commercial demands in the Indonesian market.

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Section: Discussion Discussion Discussionmentioning

confidence: 88%

Section: Discussion Discussion Discussionmentioning

confidence: 99%