Callus sieving is effective in improving synchronization and frequency of somatic embryogenesis in Citrus sinensis

Souza, J. M. M.; Tomaz, M. L.; Arruda, S. C.; Demétrio, Clarice Garcia Borges; Venables, W. N.; Martinelli, Adriana Pinheiro

doi:10.1007/s10535-011-0171-y

Cited by 16 publications

(7 citation statements)

References 30 publications

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“…In contrast, the auxin-free cultures failed to produce callus irrespective of explants used. The loosely aggregated, friable callus mass induced by the intervention of 2,4-D is capable to grow rapidly with high cell proliferation (Souza et al 2011). Due to superior response and high fresh weight, the internode derived calli were selected for further avonoid production experiments.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Callus Culture of Dianthus Chinensis L. for Assessment of Flavonoid Related Gene Expression Profile

Sreelek

Siril

2021

Preprint

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Dianthus chinensis L. is an edible, ornamental herb used to prepare the Dianthi Herba, a Chinese traditional rejuvenating medicine. Owing to the rapid proliferation of callus tissues, in vitro production of flavonoids has their own specific importance. Callus cultures raised followed by auxin directed biosynthesis of flavonoid through related transcript profile were carried out. Murashige and Skoog (MS) medium fortified with 2,4- Dichlorophenoxy acetic acid (2,4- D) or picloram induced formation of friable callus from internode derived cultures of D. chinensis. Culture medium containing 2,4- D (10 µM) produced the highest flavonoid content, 4.44 mg quercetin equivalent per gram (QE g− 1) under incubation in continuous dark condition, while maximum dry weight yield (0.38 g/ culture) was obtained from 10 µM 2,4- D under 16 h light / 8 h dark condition (50 µmol m− 2 s− 1 irradiance) at 60 days of incubation. The callus raised in light condition in 10 µM 2,4- D selected to analyze flavonoid related gene expression profile viz., chalcone synthase (CHS), chalcone isomerase (CHI), flavanone-3-hydroxylase (F3H), and flavonol synthase (FLS) at specific time intervals. The transcript abundance of CHS, F3H, or FLS gene was higher at 60 days old callus cultures and reaching 11.59, 48.31, and 114.63-fold relative expression than that of initial callus tissues respectively. These understandings are critical for the regulation of targeted phytochemicals as well as their wide exploitation in the field of biological research.

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Section: Resultsmentioning

confidence: 99%

In Vitro Callus Culture of Dianthus Chinensis L. for Assessment of Flavonoid Related Gene Expression Profile

Sreelek

Siril

2021

Preprint

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“…As mentioned by Souza et al (2014), friable calluses are distinct from compact calluses, as the former are characterized by loosely aggregated cells, with lower density and the latter are thicker aggregates of cells with higher density. The friable calluses have different cell types with different structural and histochemical characteristics, mainly characterized by the presence of cells in rapidly small growing, isodiametric, with high frequency of cell divisions (Souza et al, 2011). This kind of callus can be used to initiate cell suspension cultures, for the cells can easily disperse in the liquid medium.…”

Section: Resultsmentioning

confidence: 99%

Effect of 2,4-D and BA on the establishment of cell suspension from nodes and internodes of Capsicum annuum cv. Etna

Santos

Souza

2019

Int J Innov Educ Res

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In vitro cell suspension cultivation systems have been largely reported as safe and standardized methods for production of secondary metabolites with medicinal and agricultural interest. Capsicum annuum is one of the most widely grown vegetable in the world and its biological activities have been demonstrated against insects, fungi, bacteria and other groups of organisms. The determination of procedures for the dedifferentiation of cells into callus cells and the subsequent study of the callus growth pattern are necessary for the establishment of cell suspensions and also to subsidize studies regarding the bioactivity of its secondary metabolites. The objective of this study was to establish a protocol for dedifferentiation of leaf cells of the cultivar C. annuum cv. Etna and to determine the growth pattern of the calluses with a focus on the deceleration phase, when the callus cells must be subcultured into a liquid medium in order to establish cell suspension cultivations aiming at the production of secondary metabolites. treatment that resulted in the highest %CI, ACCC and callus weight was the combination of 4.52 µM 2,4-D + 0.44 µM BA. The calluses produced were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 23rd day of cultivation. Callus induction in leaf explants of C. annuum cv. Etna can be achieved in MS medium supplemented with 4.52 µM 2,4-D + 0.44 µM BA, which results in high cellular proliferation; in order to start a cell suspension culture, callus cells on the 23rd day of culture should be used.

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“…In angiosperms, the same diversity of developmental stages of early stage multiplying embryos can be observed. Owing to the morphology of the somatic embryos, fractionation of sizes by sieving the cultures has been shown to support synchronization in embryo cultures in angiosperms, e.g., in Daucus carota L. (Fujimura and Komamine, 1979), Citrus sinensis (L.) Osbeck (Souza et al, 2011), and Fraxinus angustifolia Vahl (Tonon et al, 2001). As the early stage embryos of a conifer PEM culture are composed of tightly associated clusters of embryos forming a web of embryonic tissue, fractionation and sieving of different stages of conifer somatic embryos are not effective.…”

Section: Overview Of Somatic Embryogenesis In In Vitro Developmental mentioning

confidence: 99%

Automation and Scale Up of Somatic Embryogenesis for Commercial Plant Production, With Emphasis on Conifers

2019

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For large scale production of clonal plants, somatic embryogenesis (SE) has many advantages over other clonal propagation methods such as the rooting of cuttings. In particular, the SE process is more suited to scale up and automation, thereby reducing labor costs and increasing the reliability of the production process. Furthermore, the plants resulting from SE closely resemble those from seeds, as somatic embryos, like zygotic (seed) embryos, develop with good connection between root and shoot, and without the plagiotropism often associated with propagation by cuttings. For practical purposes in breeding programs and for deployment of elite clones, it is valuable that a virtually unlimited number of SE plants can be generated from one original seed embryo; and SE cultures (clones) can be cryostored for at least 20 years, allowing long-term testing of clones. To date, there has however been limited use of SE for large-scale plant production mainly because without automation it is labor-intensive. Development of automation is particularly attractive in countries with high labor costs, where conifer forestry is often of great economic importance. Various approaches for automating SE processes are under investigation and the progress is reviewed here, with emphasis on conifers. These approaches include simplification of culture routines with preference for liquid rather than solid cultures, use of robotics and automation for the harvest of selected individual mature embryos, followed by automated handling of germination and subsequent planting. Different approaches to handle the processes of somatic embryogenesis in conifers are outlined below, followed by an update on efforts to automate the different steps, which are nearing an operational stage.

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Callus sieving is effective in improving synchronization and frequency of somatic embryogenesis in Citrus sinensis

Cited by 16 publications

References 30 publications

In Vitro Callus Culture of Dianthus Chinensis L. for Assessment of Flavonoid Related Gene Expression Profile

In Vitro Callus Culture of Dianthus Chinensis L. for Assessment of Flavonoid Related Gene Expression Profile

Effect of 2,4-D and BA on the establishment of cell suspension from nodes and internodes of Capsicum annuum cv. Etna

Automation and Scale Up of Somatic Embryogenesis for Commercial Plant Production, With Emphasis on Conifers

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