Calluses of Cynara cardunculus Var. Cardunculus cardoon (Asteraceae): determination of cynarine and chlorogenic acid by automated high-performance capillary electrophoresis

Trajtemberg, Silvia P.; Apóstolo, Nancy Mariel; Fernandez, Gina E.

doi:10.1079/ivp2006803

Cited by 24 publications

(9 citation statements)

References 23 publications

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“…Leaves had higher antioxidant activity (216.7 µmolTE.g -1 ) than 120-day calli (104.6 µmolTE.g -1 ). A similar result was observed in Cynara cardunculus leaves, which showed higher antioxidant activity than calli of the same species (Trajtemberg et al, 2006).…”

Section: Antioxidant Activitysupporting

confidence: 81%

Ilex paraguariensis: the effect of genotypes and growth phase on biomass, secondary metabolism and antioxidant activity of in vitro cultivated calli

Grunennvaldt¹,

Degenhardt²,

Tomasi³

et al. 2022

BLACPMA

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Yerba mate (Ilex paraguariensis) produces several secondary metabolites of interest to the pharmaceutical industry, such as chlorogenic acids and methylxanthines. These compounds have been produced in vitro by callus culture from different species. However, for I. paraguariensis, no studies upon the production of these compounds in vitro have been performed to date. In this work, we show that the concentration of secondary metabolites from I. paraguariensis callus is possible and highly dependent on the callus growth phase. We observed that the best phase for the production of secondary compounds in calli of yerba mate is the stationary growth phase on both genotypes tested. In this phase, higher levels of phenolic compounds, chlorogenic acid and 3,5-dicaffeoylquinic acid and greater antioxidant activity were observed. Chlorogenic acid and 3,5-dicaffeoylquinic acid presented positive correlation with antioxidant activity. For the first time, secondary compounds were reported in yerba mate calli cultivated in vitro.

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Section: Antioxidant Activitysupporting

confidence: 81%

Ilex paraguariensis: the effect of genotypes and growth phase on biomass, secondary metabolism and antioxidant activity of in vitro cultivated calli

Grunennvaldt¹,

Degenhardt²,

Tomasi³

et al. 2022

BLACPMA

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“…In a biorefinery context, in addition to the high yields of biomass, cardoon has been described for its rich phenolic profile, characterized by high-added value nutraceuticals [ 19 , 22 ]. Metabolic analyses revealed that cardoon calluses accumulate high levels of phenolics, in particular CGA and cynarine [ 22 , 83 ]. We confirmed the presence of high levels of total phenols in WT lines, that are even higher than previously observed in leaves of the same genotype [ 22 ], whereas total phenols were reduced in the AtMYB4oe lines, as expected.…”

Section: Discussionmentioning

confidence: 99%

Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

Paolo

Locatelli

Cominelli

et al. 2021

IJMS

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Cultivated cardoon (Cynara cardunculus var. altilis L.) is a promising candidate species for the development of plant cell cultures suitable for large-scale biomass production and recovery of nutraceuticals. We set up a protocol for Agrobacterium tumefaciens-mediated transformation, which can be used for the improvement of cardoon cell cultures in a frame of biorefinery. As high lignin content determines lower saccharification yields for the biomass, we opted for a biotechnological approach, with the purpose of reducing lignin content; we generated transgenic lines overexpressing the Arabidopsis thaliana MYB4 transcription factor, a known repressor of lignin/flavonoid biosynthesis. Here, we report a comprehensive characterization, including metabolic and transcriptomic analyses of AtMYB4 overexpression cardoon lines, in comparison to wild type, underlining favorable traits for their use in biorefinery. Among these, the improved accessibility of the lignocellulosic biomass to degrading enzymes due to depletion of lignin content, the unexpected increased growth rates, and the valuable nutraceutical profiles, in particular for hydroxycinnamic/caffeoylquinic and fatty acids profiles.

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“…In addition, several flavonoids with their glucosides (eriodictyol, eriodictyol 7 ‐O‐ glucoside, luteolin, luteolin 7‐ O ‐glucoside, trifolin, quercetin, isoquercitrin and rutin) have been detected. The co‐presence of caffeoylquinic acids, secondary metabolites present in several edible and medicinal plants from various families [35] and of cynarine, an hydroxycinnamic acid derivative, is typical of cardoon flower buds [36] . The data reported in the literature agreed on the presence of the main compounds coreopsin and sulfuretin 6 ‐O‐ glucoside, [16,25,26] while they provide different description in terms of minor compounds (eriodictyol 7 ‐O‐ glucoside, [16,26] isoquercitrin, [23,26] rutin and trifolin) [23] .…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of a South American Yellow Dye Source: Cosmos Sulphureus

2022

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Determining the dye source used in ancient coloured textiles is challenging, especially if little information is available on the raw materials or traditional recipes of the geographical area. This is the case of South American dye sources and particularly of yellow ones, the widest and highest photo‐sensitive class. In this work, Cosmos sulphureus, an annual plant whose flowers were used for dyeing in nuances of red to yellow, was analysed. The petal content was analysed by high‐pressure liquid chromatography with a diode array detector (HPLC‐DAD) and high‐resolution mass spectrometer (HPLC‐HRMS) and sixteen compounds were identified, some of which had never been reported in Cosmos sulphureus. Not mordanted and mordanted yarns, dyed with Cosmos sulphureus petal extract and artificially aged, were analysed to assess the role played by the mordant and ageing. The information pointed at Cosmos sulphureus as the dye source in a yellow Chimú / Chancay textile (1000‐1047 AD).

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Calluses of Cynara cardunculus Var. Cardunculus cardoon (Asteraceae): determination of cynarine and chlorogenic acid by automated high-performance capillary electrophoresis

Cited by 24 publications

References 23 publications

Ilex paraguariensis: the effect of genotypes and growth phase on biomass, secondary metabolism and antioxidant activity of in vitro cultivated calli

Ilex paraguariensis: the effect of genotypes and growth phase on biomass, secondary metabolism and antioxidant activity of in vitro cultivated calli

Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

Molecular Characterization of a South American Yellow Dye Source: Cosmos Sulphureus

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