Calmodulin-binding transcription activators and perspectives for applications in biotechnology

Shen, Chenjia; Yang, Yanjun; Du, Liqun; Wang, Huizhong

doi:10.1007/s00253-015-6966-6

Cited by 38 publications

(26 citation statements)

References 55 publications

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“…First, transcriptional regulators known to respond to cold in Arabidopsis, such as CBFs, were analyzed and showed an induction at 6 h with a fast decline observed by 24 h (Fig. 3A), as shown previously (Chinnusamy et al, 2003; Vogel et al, 2005; Kim et al, 2013; Shen et al, 2015; Kim et al, 2017). Overall, all of the 120 up-regulated TFs in Arabidopsis responded early to cold between 6 and 24 h at 5 °C (Fig.…”

Section: Resultssupporting

confidence: 73%

Norway spruce deploys tissue specific canonical responses to acclimate to cold

Vergara

Haas

Stachula

et al. 2020

Preprint

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SummaryCold acclimation in plants is a complex phenomenon involving numerous stress-responsive transcriptional and metabolic pathways. Existing gene expression studies have primarily addressed short-term cold acclimation responses in herbaceous plants, while few have focused on perennial evergreens, such as conifers, that survive extremely low temperatures during winter. To characterize the transcriptome changes during cold acclimation in Picea abies (L.) H. Karst (Norway spruce), we performed RNA-Sequencing analysis of needles and roots subjected to a chilling progression (5 °C) followed by 10 days at freezing temperature (−5 °C). Comparing gene expression responses of needles against Arabidopsis thaliana L. (Arabidopsis) leaves, our results showed that early transient inductions were observed in both species but the transcriptional response of Norway spruce was delayed. Our results indicate that, similar to herbaceous species, Norway spruce principally utilizes early response transcription factors (TFs) that belong to the APETALA 2/ethylene-responsive element binding factor (AP2/ERF) superfamily and NACs. However, unique to the Norway spruce response was a large group of TFs that mounted a late transcriptional response to low temperature. A predicted regulatory network analysis identified key conserved TFs, including a root-specific bHLH101 homolog and other members of the same family with a pervasive role in cold regulation, such as homologs of ICE1 and AKS3 and also homologs of the NAC (anac47 and anac28) and AP2/ERF superfamilies (DREB2 and ERF3), providing new functional insights into cold stress response strategies in Norway spruce.One sentence summaryNorway spruce shares elements of the cold regulon described in herbaceous species but has undescribed components that contribute to the cold tolerance of this evergreen coniferous species.

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Section: Resultssupporting

confidence: 73%

Norway spruce deploys tissue specific canonical responses to acclimate to cold

Vergara

Haas

Stachula

et al. 2020

Preprint

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“…Previously, while investigating the mechanisms of Ca 2+ signaling under abiotic stress, a family of six genes encoding calmodulin (CaM)-binding transcription activators (CAMTAs; also referred to as SRs) was identified in Arabidopsis (Bouché et al, 2002;Yang and Poovaiah, 2002;Mitsuda et al, 2003;Finkler et al, 2007a;Doherty et al, 2009;Shen et al, 2015), all with similar DNA-binding domains. These genes were suggested to mediate responses to various external and hormonal stimuli, including abiotic stresses and ABA (Yang and Poovaiah, 2002;Finkler et al, 2007a).…”

mentioning

confidence: 99%

CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 6: A Key Regulator of Na⁺ Homeostasis during Germination

et al. 2019

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Salinity impairs seed germination and seedling establishment. We investigated the role of Arabidopsis (Arabidopsis thaliana) CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 6 (CAMTA6) in salinity stress responses during early germination. Compared with the wild type, the camta6-4 and camta6-5 mutants were more tolerant to NaCl and abscisic acid (ABA) and accumulated less Na +. In contrast, 4-to 11-d-old camta6 seedlings were more sensitive to NaCl. In camta6, expression of HIGH-AFFINITY K + TRANSPORTER1 (AtHKT1;1), encoding an Na + /K + transporter, was restricted to the radicles and was not enhanced by NaCl or ABA. During germination, the camta6 hkt1 double mutant was as sensitive as the wild type and hkt1 to NaCl, suggesting that HKT1;1 is crucial for the salt tolerance of camta6. An ABA response element in the HKT1;1 promoter was found to be indispensable for the enhanced expression of the gene in response to NaCl and to ABA. Transcriptome analysis of the wild type and camta6-5 with and without salt treatment revealed 1,020 up-regulated and 1,467 down-regulated saltresponsive genes in the wild type. Among these, 638 up-regulated and 1,242 down-regulated genes were classified as CAMTA6-dependent. Expression of several known salt stress-associated genes, including SALT OVERLY SENSITIVE1 and Na + /H + ANTIPORTER, was impaired in camta6 mutants. Bioinformatics analysis of the 59 upstream sequences of the saltresponsive CAMTA6-dependent up-regulated genes revealed the CACGTGTC motif as the most prominent element, representing an ABA response element and a potential CAMTA-binding site. We suggest that CAMTA6 regulates, directly or indirectly, the expression of most of the salt-responsive genes in germinating seeds, including genes that are crucial for Na + homeostasis and salt stress tolerance.

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“…Arabidopsis thaliana has six CAMTA proteins that regulate the expression of genes that impart tolerance to abiotic stresses and defense against bacterial pathogens (Shen et al, 2015). The six CAMTA proteins can be divided into two classes based on the presence or absence of a TIG (transcription-associated immunoglobulin) domain (Rahman et al, 2016), which functions in nonspecific DNA binding: whereas CAMTA4, CAMTA5, and CAMTA6 have a TIG domain, CAMTA1, CAMTA2, and CAMTA3 do not.…”

Section: Introductionmentioning

confidence: 99%

CAMTA-Mediated Regulation of Salicylic Acid Immunity Pathway Genes in Arabidopsis Exposed to Low Temperature and Pathogen Infection

et al. 2017

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Arabidopsis thaliana calmodulin binding transcription activator (CAMTA) factors repress the expression of genes involved in salicylic acid (SA) biosynthesis and SA-mediated immunity in healthy plants grown at warm temperature (22°C). This repression is overcome in plants exposed to low temperature (4°C) for more than a week and in plants infected by biotrophic and hemibiotrophic pathogens. Here, we present evidence that CAMTA3-mediated repression of SA pathway genes in nonstressed plants involves the action of an N-terminal repression module (NRM) that acts independently of calmodulin (CaM) binding to the IQ and CaM binding (CaMB) domains, a finding that is contrary to current thinking that CAMTA3 repression activity requires binding of CaM to the CaMB domain. Induction of SA pathway genes in response to low temperature did not occur in plants expressing only the CAMTA3-NRM region of the protein. Mutational analysis provided evidence that the repression activity of the NRM was suppressed by action of the IQ and CaMB domains responding to signals generated in response to low temperature. Plants expressing the CAMTA3-NRM region were also impaired in defense against the bacterial hemibiotrophic pathogen Pseudomonas syringae pv tomato DC3000. Our results indicate that the regulation of CAMTA3 repression activity by low temperature and pathogen infection involves related mechanisms, but with distinct differences.

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Calmodulin-binding transcription activators and perspectives for applications in biotechnology

Cited by 38 publications

References 55 publications

Norway spruce deploys tissue specific canonical responses to acclimate to cold

Norway spruce deploys tissue specific canonical responses to acclimate to cold

CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 6: A Key Regulator of Na⁺ Homeostasis during Germination

CAMTA-Mediated Regulation of Salicylic Acid Immunity Pathway Genes in Arabidopsis Exposed to Low Temperature and Pathogen Infection

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Calmodulin-binding transcription activators and perspectives for applications in biotechnology

Cited by 38 publications

References 55 publications

Norway spruce deploys tissue specific canonical responses to acclimate to cold

Norway spruce deploys tissue specific canonical responses to acclimate to cold

CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 6: A Key Regulator of Na+ Homeostasis during Germination

CAMTA-Mediated Regulation of Salicylic Acid Immunity Pathway Genes in Arabidopsis Exposed to Low Temperature and Pathogen Infection

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CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 6: A Key Regulator of Na⁺ Homeostasis during Germination