Calogênese, curva de crescimento de calos e formação de suspensões celulares de Lavandula

Abstract

The cultivation of calli and cell suspension lines of aromatic plants is important because they are considered to be factories of active compounds such as, for instance, the rosmarinic acid found in Lavandula species. L. angustifolia is considered the most important species because it is regarded to be practically free from camphor, differently from other species in the genus, however, it is less productive than L. dentata, which is very productive and easily asexually propagated, but presents higher content of camphor. Information about calli and cell suspensions induction and maintenance for Lavandula species, especially the latter, is scarce. Not only that, but once callus formation is induced, it is necessary to identify the growth dynamic of the cells in order to maintain healthy, logarithmically phased growing cells for future elicitation. The goals of this work were to induce calli formation, evaluate growth curve and produce cell suspensions of Lavandula angustifolia and Lavandula dentata, two Lavandula species of great economical importance. Apical and lateral buds of L. angustifolia and L. dentata were placed on MS + 1 mg.L -1 2.4-D or picloran + 0.5 mg.L -1 BAP or 1 mg.L -1 2.4-D + 0.5 mg.L -1 GA 3 , in the presence of light or in the dark. Calli were obtained only from L. angustifolia, under light, with the combination of 2.4-D + GA 3 . The growth curve of L. angusifolia calli indicated the need for media exchange every three weeks. A blue pigment which may be interesting as a dye in the food industry was present in the media. Cells suspensions were formed.Key words: Growth regulator, lavender, micropropagation, tissue culture. Calogênese, curva de crescimento de calos e formação de suspensões celulares de Lavandula RESUMO O cultivo de calos e suspensões celulares de plantas aromáticas é importante porque estes são considerados fábricas de compostos ativos como, por exemplo, o ácido rosmarínico encontrado em espécies de Lavandula. L. angustifolia é considerada a espécie mais importante por ser praticamente livre de cânfora, todavia, é menos produtiva que L. dentata, que é facilmente propagada vegetativamente, mas apresenta alto teor de cânfora. Informação sobre indução e manutenção de calos e suspensões celulares de espécies de Lavandula, especialmente a última, é escassa. Não somente isto, mas uma vez que a formação de calos é induzida, é necessário identificar o crescimento dinâmico das células para mantê-las com crescimento saudável, em fase logarítmica para futuras elicitações. Os alvos deste trabalho foram indução de calos, avaliação de curva de crescimento e formação de suspensões celulares de Lavandula angustifolia e Lavandula dentata, duas espécies de Lavandula de grande importância econômica. Gemas de L. angustifolia e L. dentata foram colocadas em MS + 1 mg.L -1 2,4-D ou picloran + 0,5 mg.L -1 BAP ou 1 mg.L -1 2,4-D + 0,5 mg.L -1 GA 3 , em condições de luz, ou no escuro. Foram obtidos calos de L. angustifolia em condições de luz, sendo 2,4-D + GA 3 a melhor combinação. A curva de cr...

Indirect in vitro organogenesis of Hancornia speciosa Gomes

“…has also showed the highest percentage of callus formation (98%) with further shooting regeneration in the culture medium supplied with 6.0 µM 2,4-D and 0.5 µM TDZ (BASKARAN et al, 2009) which demonstrates that the presence of 2,4-D was crucial to obtain the result. The superiority of this synthetic auxin in comparison to other auxin and cytokinin sources during indirect organogenesis has also been recorded in essays with Lavandula angustifolia buds in which the use of 4.52 µM 2,4-D and 1.44 µM GA 3 in the culture medium was the most suitable combination to induce calluses (BONA et al, 2012), as well as for leaf segments of Myracrodruon urundeuva in which the use of 4.52 µM 2,4-D promoted the highest rate of callus formation (VASCONCELOS et al, 2012). The importance of the auxins as an inductor of the cell competence is known and the 2,4-D has been particularly reported in literature as a necessary regulator for the somatic cell dedifferentiation (RAGHAVAN, 2004;PINTO et al, 2010;LI et al, 2012) most of all from parenchyma cells of vascular bundles (LI et al, 2012).…”