Calorespirometry of terrestrial organisms and ecosystems

Wadsö, Lars; Hansen, Lee D.

doi:10.1016/j.ymeth.2014.10.024

Cited by 29 publications

(19 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, the maximum measured heat flow using IMC matches well with calculated heat flow for M. smegmatis and M. tuberculosis (Table 2 ). Furthermore, the calculated heat flow based on carbon dioxide production rate of both mature biofilms confirm the fact that 50 µmol s −1 CO 2 lead to 5 µW heat production rate 38 .…”

Section: Discussionsupporting

confidence: 71%

Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria

Solokhina

Bruckner

Bonkat³

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Mycobacteria are classified into two groups, fast- and slow-growing. Often, fast-growing mycobacteria are assumed to have a higher metabolic activity than their slower counterparts, but in mature biofilms this assumption might not be correct. Indeed, when measuring the metabolic activity of mycobacterial biofilms with two independent non-invasive techniques (isothermal microcalorimetry and tunable diode laser absorption spectrometry), mature biofilms of slow- and fast-growing species appeared more alike than expected. Metabolic heat production rate was 2298 ± 181 µW for M. smegmatis and 792 ± 81 µW for M. phlei, while M. tuberculosis and M. bovis metabolic heat production rates were between these values. These small differences were further confirmed by similar oxygen consumption rates (3.3 ± 0.2 nMole/s and 1.7 ± 0.3 nMole/s for M. smegmatis and M. tuberculosis, respectively). These data suggest that the metabolic potential of slow-growing mycobacterial biofilms has been underestimated, particularly for pathogenic species.

show abstract

Section: Discussionsupporting

confidence: 71%

Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria

Solokhina

Bruckner

Bonkat³

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…However, if a product is formed, an additional online measure is required. Therefore, we took advantage of calorespirometry, a rarely used, but highly informative technique (Wadsö and Hansen, ). Many metabolic events, for example, shifts from one substrate to another, or occurrence of nutrient limitations, lead to a characteristic change in the calorespirometric ratios, heat per CO 2 and heat per O 2 .…”

Section: Resultsmentioning

confidence: 99%

Calorespirometric feeding control enhances bioproduction from toxic feedstocks—Demonstration for biopolymer production out of methanol

Rohde

Paufler

Harms

et al. 2016

Biotech & Bioengineering

View full text Add to dashboard Cite

The sustainable production of fuels and industrial bulk chemicals by microorganisms in biotechnological processes is promising but still facing various challenges. In particular, toxic substrates require an efficient process control strategy. Methanol, as an example, has the potential to become a major future feedstock due to its availability from fossil and renewable resources. However, besides being toxic, methanol is highly volatile. To optimize its dosage during microbial cultivations, an innovative, predictive process control strategy based on calorespirometry, i.e., simultaneous measurements of heat and CO2 emission rates, was developed. This rarely used technique allows an online-estimation of growth parameters such as the specific growth rate and substrate consumption rate as well as a detection of shifts in microbial metabolism thus enabling an adapted feeding for different phases of growth. The calorespirometric control strategy is demonstrated exemplarily for growth of the methylotrophic bacterium Methylobacterium extorquens on methanol and compared to alternative control strategies. Applying the new approach, the methanol concentration could be maintained far below a critical limit, while increased growth rates of M. extorquens and higher final contents of the biopolymer polyhydroxybutyrate were obtained. Biotechnol. Bioeng. 2016;113: 2113-2121. © 2016 Wiley Periodicals, Inc.

show abstract

“…Calorespirometry permits the direct measurement of the metabolic heat and CO 2 dissipation rates of any living system including soil (Wadsö and Hansen 2015, Barros et al 2016). The quotient of the heat rate and CO 2 rate may approach the enthalpy change for catabolism of SOM.…”

Section: Introductionmentioning

confidence: 99%

Thermodynamics of soil organic matter decomposition in semi‐natural oak (Quercus) woodland in southwest Ireland

Barros

Fernández

Byrne

et al. 2020

Oikos

Self Cite

View full text Add to dashboard Cite

The evolution of soil terrestrial ecosystems is a subject with difficulties to define their maturity and evolutionary state. In the last century, thermodynamics was one of the options considered by ecologists for that goal. Difficulties in quantifying the thermodynamic parameters needed by the evolutionary theories caused that this subject has been practically locked since the end of the last century. Application of thermodynamics needs reactions and one of the main reactions in soil ecosystems are those involved in the decomposition of the soil organic matter. This paper aims to provide an initial step to study those reactions from a thermodynamic perspective. With that goal in mind, thermal analysis and isothermal calorespirometric measurements were made on soil samples collected at three depths in semi‐natural oak woodlands at three different sites in southwest Ireland. It is assumed that the organic matter evolves from a less to a higher mature state as soil depth increases. The maturity state could be chemically defined by the redox state. The proposed methods yield the enthalpy change, Gibbs energy change and entropy change for the microbial catabolism and combustion reactions of the soil organic matter. The degree of reduction was calculated by the enthalpy changes. Results show the soil organic matter becomes more reduced from the soil organic surface to mineral soils. The top layer is characterized by high carbon content, organic materials with low energy content per Cmole, and fast biodegradation rates. Mineral soils are characterized by low carbon content, organic materials with high energy content per Cmole, and slow biodegradation rates. Values obtained for the entropy change were sensitive to these differences among the different soil layers. These results contribute to unlock the thermodynamics of the soil reactions and to develop the bioenergetics of soil ecosystems.

show abstract

Calorespirometry of terrestrial organisms and ecosystems

Cited by 29 publications

References 48 publications

Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria

Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria

Calorespirometric feeding control enhances bioproduction from toxic feedstocks—Demonstration for biopolymer production out of methanol

Thermodynamics of soil organic matter decomposition in semi‐natural oak (Quercus) woodland in southwest Ireland

Contact Info

Product

Resources

About