Caloric state modulates locomotion, heart rate and motor neuron responses to acute administration of d-amphetamine in zebrafish larvae

Bansal, Pushkar; Roitman, Mitchell F.; Jung, Erica E.

doi:10.1016/j.physbeh.2023.114144

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…Its small size (∼3mm) makes it easy to house for raising and handling during experimentation. Zebrafish larvae are optically transparent, which is advantageous in imaging and recording the activities inside the larval body, such as in the heart and brain (Bansal et al, 2023; Keller & Ahrens, 2015). We leveraged all these useful features, including optical transparency in the larvae, and recorded the change in DA neuron activity at a single neuron level using calcium imaging in vivo in real-time.…”

Section: Introductionmentioning

confidence: 99%

Hypothalamic dopamine neuron activity is modulated by caloric states and amphetamine abuse in zebrafish larvae

Bansal,

Jung

2024

Preprint

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Dopaminergic (DA) neuron activity is affected by reward and stress-inducing entities such as food and drugs of abuse, and different DA neuron populations can respond distinctly to these stimuli. Interaction between both stimuli significantly alters the dynamics of DA release in different DA populations. Additionally, these stimulating entities can affect the interconnections among different DA populations by impairing their correspondence with each other. However, limited studies have been performed that could point to the effect of interaction between AMPH and caloric states on DA neurons and their inter-correlation. This study explores the individual and interactive effect of two caloric states, ad-libitum fed (AL) and food deprived (FD), and acute exposure to a stimulant drug (amphetamine) in two different DA neurons in the hypothalamus of zebrafish larvae. We used a transgenic zebrafish line Tg(th2:GCaMP7s), which expresses a calcium indicator (GCaMP7s) in A11(Posterior Tuberculum) and a part of A14 (Caudal Hypothalamus and Intermediate Hypothalamus) DA populations located in the hypothalamus of the larval zebrafish. The larvae were subjected to acute FD and ad AL feeding followed by acute treatment with 0.7uM and 1.5uM doses of AMPH. We recorded calcium activity and quantified fluorescence change, activity duration, peak rise/fall time, and latency in the spikes of the DA neurons. Our results show that baseline DA neuron activity amplitude, spike duration, and correlation between inter- and intra-DA neurons were higher in the FD than in the AL state. Dose-dependent AMPH treatment further increased the activity intensity of the aforementioned parameters in the neuron spikes in the FD state. The DA activity correlation and spike latency were dose-dependently impaired in both DA populations. These results suggest that different DA populations in the brain exhibit a similar activity trend in response to caloric states and AMPH, where the AMPH-mediated intensity change in the activity was dose-dependent.

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Section: Introductionmentioning

confidence: 99%

Hypothalamic dopamine neuron activity is modulated by caloric states and amphetamine abuse in zebrafish larvae

Bansal,

Jung

2024

Preprint

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“…Larval zebrafish have shown behavioral sensitivity similar to mammals to a variety of addictive drugs including amphetamine(Irons et al, 2010; Cousin et al, 2014; Basnet et al, 2019). A recent study showed the effect of chronic food restriction on physiological responses, locomotion, heart rate, and motor neuron response(Bansal et al, 2023). Here, we address whether acute feeding and acute deprivation can differently modulate the motor response to amphetamine especially relative to the ad libitum fed and chronically deprived state.…”

Section: Introductionmentioning

confidence: 99%

Modulation of locomotion and motor neuron response by the cohesive effect of acute and chronic feeding states and acute d-amphetamine treatment in zebrafish larvae

Bansal,

Roitman,

Jung

2024

Preprint

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Amphetamine (AMPH) increases locomotor activities in animals, and the locomotor response to AMPH is further modulated by caloric deficits such as food deprivation and restriction. The increment in locomotor activity regulated by AMPH-caloric deficit concomitance can be further modulated by varying feeding schedules (e.g. acute and chronic food deprivation and acute feeding after chronic food deprivation). However, the effects of different feeding schedules on AMPH-induced locomotor activity are yet to be explicated. Here, we have explored the stimulatory responses of acutely administered d-amphetamine in locomotion under systematically varying feeding states (fed/sated and food deprivation) and schedules (chronic and acute) in zebrafish larvae. We used wild-type and transgenic [Tg(mnx1:GCaMP5)] zebrafish larvae and measured swimming activity and spinal motor neuron activity in vivo in real-time in time-elapsed and cumulative manner pre- and post-AMPH treatment. Our results showed that locomotion and motor neuron activity increased in both chronic and acute food deprivation post-AMPH treatment cumulatively. A steady increase in locomotion was observed in acute food-deprivation compared to an immediate abrupt increase in chronic food-deprivation state. The ad libitum-fed larvae exhibited a moderate increase both in locomotion and motor neuron activity. Conversely to all other caloric states, food-sated (acute feeding after chronic food deprivation) larvae moved moderately less and exhibited a mild decrease in motor neuron activity after AMPH treatment. These results point to the importance of the feeding schedule in modulating characteristic stimulatory response of amphetamine on behavior and motor neurons.

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“…The quantitative analysis of zebrafish behavioral phenotypes can serve as a valuable tool for studying drug effects and mechanisms of action [7][8][9][10][11][12]. Additionally, it can provide insights into the psychological state of zebrafish [13].…”

Section: Introductionmentioning

confidence: 99%

Quantifying Variability in Zebrafish Larvae Locomotor Behavior across Experimental Conditions: A Learning-Based Tracker

Zhang,

Chai,

et al. 2024

Fishes

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This study investigated the effects of environmental changes on zebrafish larval behavior, using single-factor and orthogonal experiments to assess locomotion during temperature and pH changes. In single-factor experiments, zebrafish larvae were exposed to variations in temperature (22 to 30 °C) and pH levels (6.0, 7.0, 9.0). The simultaneous temperature and pH changes were investigated by orthogonal tests. In both experiments, each zebrafish larva was recorded in three 5 min videos at different stages (before exposure, during short-term exposure (10 min), and after long-term exposure (60 min)). You Look Only Once (YOLOv5) and Deep Simple Online Real Time Tracking (DeepSORT) models were adopted to develop a zebrafish larva tracking system, and YOLOv5 was improved in two aspects of anchor clustering and network structure. The tracking accuracy of the tracking system for small targets effectively improved, reaching more than 98% MOTA (Multiple Object Tracking Accuracy). Principal Component Analysis (PCA) was employed to extract three behavioral features from 13 motion parameters, namely motion activity, edge behavior, and motion direction preference. Our findings reveal that lower temperatures and acidic conditions both led to a decrease in motion behavioral activity, and the former also increased edge behavior. Conversely, elevated temperatures and alkaline conditions had a muted impact on these behaviors. Interestingly, concurrent changes in temperature and pH significantly altered directional preference. Additionally, we observed that lower temperatures elicited distinct temporal behavioral patterns at a constant pH level. In summary, we recommend the precise control and explicit reporting of ambient temperature and pH in both breeding devices and experimental wells to minimize the environmental impact on zebrafish behavior and enhance experiment repeatability and reliability.

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Caloric state modulates locomotion, heart rate and motor neuron responses to acute administration of d-amphetamine in zebrafish larvae

Cited by 6 publications

References 42 publications

Hypothalamic dopamine neuron activity is modulated by caloric states and amphetamine abuse in zebrafish larvae

Hypothalamic dopamine neuron activity is modulated by caloric states and amphetamine abuse in zebrafish larvae

Modulation of locomotion and motor neuron response by the cohesive effect of acute and chronic feeding states and acute d-amphetamine treatment in zebrafish larvae

Quantifying Variability in Zebrafish Larvae Locomotor Behavior across Experimental Conditions: A Learning-Based Tracker

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