Calorie restriction changes muscle satellite cell proliferation in a manner independent of metabolic modulation

Abreu, Phablo; Serna, Julian David C.; Munhoz, Ana Cláudia; Kowaltowski, Alicia J.

doi:10.1016/j.mad.2020.111362

Cited by 9 publications

(7 citation statements)

References 24 publications

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“…CR has also the potential to modulate satellite cells, the stem cells of skeletal muscle, which are involved in muscle homeostasis and regeneration [ 27 , 28 ]. Some reports suggest that CR increases the number of satellite cells and enhances their regenerative ability [ 27 , 29 , 30 ]. Cerletti et al [ 29 ] showed that satellite cells from young mice under CR were metabolically reprogrammed and presented increased number of mitochondria and oxidative phosphorylation.…”

Section: Discussionmentioning

confidence: 99%

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Sex Specific Differences in Response to Calorie Restriction in Skeletal Muscle of Young Rats

et al. 2022

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Calorie restriction (CR), defined as a reduction of the total calorie intake of 30% to 60% without malnutrition, is the only nutritional strategy that has been shown to extend lifespan, prevent or delay the onset of age-associated diseases, and delay the functional decline in a wide range of species. However, little is known about the effects of CR when started early in life. We sought to analyze the effects of CR in the skeletal muscle of young Wistar rats. For this, 3-month-old male and female rats were subjected to 40% CR or fed ad libitum for 3 months. Gastrocnemius muscles were used to extract RNA and total protein. Western blot and RT-qPCR were performed to evaluate the expression of key markers/pathways modulated by CR and affected by aging. CR decreased body and skeletal muscle weight in both sexes. No differences were found in most senescence, antioxidant, and nutrient sensing pathways analyzed. However, we found a sexual dimorphism in markers of oxidative stress, inflammation, apoptosis, and mitochondrial function in response to CR. Our data show that young female rats treated with CR exhibit similar expression patterns of key genes/pathways associated with healthy aging when compared to old animals treated with CR, while in male rats these effects are reduced. Additional studies are needed to understand how early or later life CR exerts positive effects on healthspan and lifespan.

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Section: Discussionmentioning

confidence: 99%

“…Cerletti et al [ 29 ] showed that satellite cells from young mice under CR were metabolically reprogrammed and presented increased number of mitochondria and oxidative phosphorylation. However, Abreu et al [ 30 ] reported that the increased proliferation of satellite cells of CR mice is not consequence of a change in metabolism.…”

Section: Discussionmentioning

confidence: 99%

Sex Specific Differences in Response to Calorie Restriction in Skeletal Muscle of Young Rats

et al. 2022

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“…However, mutant satellite cells cultivated with serum from Adck2 +/− mice on CR differentiated faster and showed myotube differentiation similar to that of WT satellite cells in the same conditions ( Figures 6B,C ), which is directly associated with the increase in oxidative pathways with better fatty acids use. During differentiation of adult muscle stem cells, a progressive metabolic shift from glycolysis to oxidative phosphorylation occurs through the activation of mitochondria and oxidative pathways ( Relaix et al, 2021 ), although other factors could also be involved ( Abreu et al, 2020 ). It has been previously shown that the addition of mammalian CR serum to cell cultures could result in a higher tolerance to stress response, reduced cell proliferation and lower oxidative damage due to components present in the serum ( De Cabo et al, 2003 ; García-Matas et al, 2015 ; Novelle et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Calorie Restriction Rescues Mitochondrial Dysfunction in Adck2-Deficient Skeletal Muscle

et al. 2022

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ADCK2 haploinsufficiency-mediated mitochondrial coenzyme Q deficiency in skeletal muscle causes mitochondrial myopathy associated with defects in beta-oxidation of fatty acids, aged-matched metabolic reprogramming, and defective physical performance. Calorie restriction has proven to increase lifespan and delay the onset of chronic diseases associated to aging. To study the possible treatment by food deprivation, heterozygous Adck2 knockout mice were fed under 40% calorie restriction (CR) and the phenotype was followed for 7 months. The overall glucose and fatty acids metabolism in muscle was restored in mutant mice to WT levels after CR. CR modulated the skeletal muscle metabolic profile of mutant mice, partially rescuing the profile of WT animals. The analysis of mitochondria isolated from skeletal muscle demonstrated that CR increased both CoQ levels and oxygen consumption rate (OCR) based on both glucose and fatty acids substrates, along with mitochondrial mass. The elevated aerobic metabolism fits with an increase of type IIa fibers, and a reduction of type IIx in mutant muscles, reaching WT levels. To further explore the effect of CR over muscle stem cells, satellite cells were isolated and induced to differentiate in culture media containing serum from animals in either ad libitum or CR diets for 72 h. Mutant cells showed slower differentiation alongside with decreased oxygen consumption. In vitro differentiation of mutant cells was increased under CR serum reaching levels of WT isolated cells, recovering respiration measured by OCR and partially beta-oxidation of fatty acids. The overall increase of skeletal muscle bioenergetics following CR intervention is paralleled with a physical activity improvement, with some increases in two and four limbs strength tests, and weights strength test. Running wheel activity was also partially improved in mutant mice under CR. These results demonstrate that CR intervention, which has been shown to improve age-associated physical and metabolic decline in WT mice, also recovers the defective aerobic metabolism and differentiation of skeletal muscle in mice caused by ADCK2 haploinsufficiency.

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“…Caloric restriction has been shown to extend lifespan and ameliorate age-related diseases in most species, and can have beneficial effects on mitochondrial function and apoptotic signaling in skeletal muscle of both young and old animals (Cerletti et al, 2012). Statistics show that even a reduction in food intake of as little as 8% still provides protection against sarcopenia (Marzetti et al, 2008), and both short-and long-term caloric restriction enhances myogenic cell proliferation (Abreu et al, 2020). Activation of mTOR signaling is one of the mechanisms of aging and many studies have confirmed that rapamycin can improve agingrelated diseases and effectively prolong lifespan by inhibiting the mTOR pathway.…”

Section: Strategies Acting On the Environmentmentioning

confidence: 99%

Contribution of muscle satellite cells to sarcopenia

Huo

Liu

2022

Front. Physiol.

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Sarcopenia, a disorder characterized by age-related muscle loss and reduced muscle strength, is associated with decreased individual independence and quality of life, as well as a high risk of death. Skeletal muscle houses a normally mitotically quiescent population of adult stem cells called muscle satellite cells (MuSCs) that are responsible for muscle maintenance, growth, repair, and regeneration throughout the life cycle. Patients with sarcopenia are often exhibit dysregulation of MuSCs homeostasis. In this review, we focus on the etiology, assessment, and treatment of sarcopenia. We also discuss phenotypic and regulatory mechanisms of MuSC quiescence, activation, and aging states, as well as the controversy between MuSC depletion and sarcopenia. Finally, we give a multi-dimensional treatment strategy for sarcopenia based on improving MuSC function.

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Calorie restriction changes muscle satellite cell proliferation in a manner independent of metabolic modulation

Cited by 9 publications

References 24 publications

Sex Specific Differences in Response to Calorie Restriction in Skeletal Muscle of Young Rats

Sex Specific Differences in Response to Calorie Restriction in Skeletal Muscle of Young Rats

Calorie Restriction Rescues Mitochondrial Dysfunction in Adck2-Deficient Skeletal Muscle

Contribution of muscle satellite cells to sarcopenia

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