Calorimetric real time monitoring of lambda prophage induction

Maskow, Thomas; Kiesel, Bärbel; Schubert, Torsten; Zhou, Yong; Harms, Hauke; Yao, Jun

doi:10.1016/j.jviromet.2010.05.002

Cited by 31 publications

(31 citation statements)

References 36 publications

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“…Recently, Lucia insists on applying thermodynamic methodology in research on biological processes, including viral life cycle and epidemiology [ Lucia and Grisolia, 2020 ; Lucia, 2015 ; Lucia et al, 2021 , 2020a , 2020b ; Kaniadakis et al, 2020 ]. Maskow has used thermodynamics and calorimetry to study microorganisms and viruses [ Maskow, 2013 ; Maskow et al, 2010a , 2010b ].…”

Section: Introductionmentioning

confidence: 99%

Beyond COVID-19: Do biothermodynamic properties allow predicting the future evolution of SARS-CoV-2 variants?

Popovic

2022

Microbial Risk Analysis

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Section: Introductionmentioning

confidence: 99%

Beyond COVID-19: Do biothermodynamic properties allow predicting the future evolution of SARS-CoV-2 variants?

Popovic

2022

Microbial Risk Analysis

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“…As every metabolic flux is quantitatively related to the heat production rate via the law of Hess, the heat signal can provide a global real time insight into the stoichiometry and kinetics of bioprocesses (von Stockar and Marison, ). Biocalorimetry is applicable to any microbial system, for instance, bacteria (Ishikawa and Shoda, ), yeasts (Larsson et al, ), animal cell cultures (Guan et al, ), and even for monitoring phage‐bacteria interactions (Maskow et al, ). Other advantages of this technique are its non‐invasiveness and high sensitivity, and the facts that it delivers real time signals, works in optically opaque solutions, and does not require labeling or specific reactants.…”

Section: Introductionmentioning

confidence: 99%

Calorespirometric feeding control enhances bioproduction from toxic feedstocks—Demonstration for biopolymer production out of methanol

Rohde

Paufler

Harms

et al. 2016

Biotech & Bioengineering

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The sustainable production of fuels and industrial bulk chemicals by microorganisms in biotechnological processes is promising but still facing various challenges. In particular, toxic substrates require an efficient process control strategy. Methanol, as an example, has the potential to become a major future feedstock due to its availability from fossil and renewable resources. However, besides being toxic, methanol is highly volatile. To optimize its dosage during microbial cultivations, an innovative, predictive process control strategy based on calorespirometry, i.e., simultaneous measurements of heat and CO2 emission rates, was developed. This rarely used technique allows an online-estimation of growth parameters such as the specific growth rate and substrate consumption rate as well as a detection of shifts in microbial metabolism thus enabling an adapted feeding for different phases of growth. The calorespirometric control strategy is demonstrated exemplarily for growth of the methylotrophic bacterium Methylobacterium extorquens on methanol and compared to alternative control strategies. Applying the new approach, the methanol concentration could be maintained far below a critical limit, while increased growth rates of M. extorquens and higher final contents of the biopolymer polyhydroxybutyrate were obtained. Biotechnol. Bioeng. 2016;113: 2113-2121. © 2016 Wiley Periodicals, Inc.

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“…Numerous lytic phages were isolated against Caulobacter and Asticcacaulis bacteria using the enrichment method [25]. Methods such as spot testing, plaque testing, culture lysis and the calorimetric method are used in the detection of newly isolated bacteriophages ( Figure 2) [26][27][28][29][30][31]. In the spot assay [26], bacteria are grown in Luria-Bertani broth, and after they are in the early log phase, they are mixed with soft agar and poured onto a Petri plate with previously poured agar.…”

Section: Phages As Pharmaceuticals 21 Phage Isolation and Enrichmentmentioning

confidence: 99%

Bacteriophage Therapy of Bacterial Infections: The Rediscovered Frontier

2021

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Antibiotic-resistant infections present a serious health concern worldwide. It is estimated that there are 2.8 million antibiotic-resistant infections and 35,000 deaths in the United States every year. Such microorganisms include Acinetobacter, Enterobacterioceae, Pseudomonas, Staphylococcus and Mycobacterium. Alternative treatment methods are, thus, necessary to treat such infections. Bacteriophages are viruses of bacteria. In a lytic infection, the newly formed phage particles lyse the bacterium and continue to infect other bacteria. In the early 20th century, d’Herelle, Bruynoghe and Maisin used bacterium-specific phages to treat bacterial infections. Bacteriophages are being identified, purified and developed as pharmaceutically acceptable macromolecular “drugs,” undergoing strict quality control. Phages can be applied topically or delivered by inhalation, orally or parenterally. Some of the major drug-resistant infections that are potential targets of pharmaceutically prepared phages are Pseudomonas aeruginosa, Mycobacterium tuberculosis and Acinetobacter baumannii.

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Calorimetric real time monitoring of lambda prophage induction

Cited by 31 publications

References 36 publications

Beyond COVID-19: Do biothermodynamic properties allow predicting the future evolution of SARS-CoV-2 variants?

Beyond COVID-19: Do biothermodynamic properties allow predicting the future evolution of SARS-CoV-2 variants?

Calorespirometric feeding control enhances bioproduction from toxic feedstocks—Demonstration for biopolymer production out of methanol

Bacteriophage Therapy of Bacterial Infections: The Rediscovered Frontier

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