Calorimetric study of adsorption of human serum albumin onto silica powders

Kulikova, Galina A.; Ryabinina, I. V.; Guseynov, Sabir S.; Parfenyuk, Elena V.

doi:10.1016/j.tca.2010.03.009

Cited by 10 publications

(9 citation statements)

References 19 publications

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“…Their density is pH-dependent, with a p K a of about 5 . Thus, serum albumin (net negative) adsorbs to silica with a monolayer coverage of about 1 mg m –2 even though the adsorption is endothermic . The adsorption is driven entropically by the release of counterions .…”

Section: Resultsmentioning

confidence: 99%

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Quasi-Spherical Cell Clusters Induced by a Polyelectrolyte Multilayer

2015

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Fibroblasts cultured on polyelectrolyte multilayers, PEMUs, made from poly(diallyldimethylammonium), PDADMA, and poly(styrene sulfonate), PSS, showed a variety of attachment modes, depending on the charge of the last layer and deposition conditions. PEMUs terminated with PDADMA (cationic) were cytotoxic when built in 1.0 M NaCl but cytophilic when built in 0.15 M NaCl. Cells adhered poorly to all PSS-capped (anionic) films. PEMUs built in 0.15 M NaCl but terminated with a layer of PSS in 1.0 M NaCl induced most cells to form spherical clusters after about 48 h of culture. These clusters still interrogated the surface, and when they were replated on control tissue culture plastic, cells emerged with close to 100% viability. Differences between the various surfaces were probed in an effort to identify the mechanism responsible for this unusual behavior, which did not follow accepted correlations between substrate stiffness and cell adhesion. No significant differences in roughness or wetting were observed between cluster-inducing PSS-capped multilayers and those that did not produce clusters. When the surface charge was assayed with radiolabeled ions a strong increase in negative surface charge was revealed. Viewing the multilayer as a zwitterionic solid and comparing its surface charge density to that of a cell membrane yields similarities that suggest a mechanism for preventing protein adhesion to the surface, a necessary step in the integrin-mediated mechanotransduction properties of a cell.

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Section: Resultsmentioning

confidence: 99%

“…56 Thus, serum albumin (net negative) adsorbs to silica with a monolayer coverage of about 1 mg m −2 even though the adsorption is endothermic. 57 The adsorption is driven entropically by the release of counterions. 54 If the phospholipid headgroup occupies about 60 Å 2 , the density of lipids at the cell membrane is 2.8 μmol m −2 .…”

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confidence: 99%

Quasi-Spherical Cell Clusters Induced by a Polyelectrolyte Multilayer

2015

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“… 137 Thermochemical measurements of serum albumin adsorption to silica reveal an endothermic signature. 138 The electrostatic attraction of a positive patch on the protein to the negative surface should be exothermic. Because adsorption is spontaneous, the driving force must include an entropic component that outweighs the positive enthalpic one.…”

Section: Introductionmentioning

confidence: 99%

“…For example, silica is a strongly hydrophilic surface (water contact angle in air of ∼0°), yet silica is a universal adsorber for proteins . Thermochemical measurements of serum albumin adsorption to silica reveal an endothermic signature . The electrostatic attraction of a positive patch on the protein to the negative surface should be exothermic.…”

Section: Introductionmentioning

confidence: 99%

Zwitteration: Coating Surfaces with Zwitterionic Functionality to Reduce Nonspecific Adsorption

2014

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Coating surfaces with thin or thick films of zwitterionic material is an effective way to reduce or eliminate nonspecific adsorption to the solid/liquid interface. This review tracks the various approaches to zwitteration, such as monolayer assemblies and polymeric brush coatings, on micro- to macroscopic surfaces. A critical summary of the mechanisms responsible for antifouling shows how zwitterions are ideally suited to this task.

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“…″Template bleeding″ is a phenomenon of slow release of the template in small quantities coming from leakage of a physically entrapped template facilitated by the swelling and shrinkage of the polymer material, or formation of template clusters that could be released by the polymeric material. , Generally, for low-molecular-weight templates, highly cross-linked polymers are used to ensure preservation of the imprint cavity after its removal. Conversely, for a larger template, high cross-link densities can affect the mass transfer of the template, affect rebinding kinetics, and lead to slow template removal and thorough washing setps , or even permanent entrapment in the polymer network . Even though advanced techniques like thermal annealing, microwave-assisted extraction, Soxhlet extraction, and super critical fluid template desorption , have been used to recover the template from imprinted polymer, complete removal of template is often not possible.…”

Section: Discussionmentioning

confidence: 99%

Synthesis, Evaluation, and Characterization of an Ergotamine Imprinted Styrene-Based Polymer for Potential Use as an Ergot Alkaloid Selective Adsorbent

et al. 2021

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Alkaloid toxicities negatively impact livestock health and production. To assess alkaloid occurrences, adsorbent technologies may offer effective means to their extraction and isolation from a complex feed matrix. In this study, molecularly imprinted polymers (MIPs) were synthesized and evaluated for their specificity of binding to various ergot alkaloids. Co-polymers of styrene and hydroxyethyl methacrylate were synthesized in the absence or presence of an ergotamine (ETA) template, yielding non-imprinted polymer (NIP) and molecularly imprinted polymer (MIP), respectively. The influence of parameters such as pH, temperature, and initial concentration on the adsorption of ergot alkaloids was evaluated along with their application as solid phase extraction materials. Chemical and morphological properties were characterized. Adsorption was generally greater for MIP compared to NIP. Cross-reactivity with related alkaloids existed due to similarities in structure and functional groups and was dependent on the type and concentration of alkaloid and polymer type (alkaloid type × concentration × product; P < 0.05). The pH of the medium had no influence on the binding properties of polymers toward ETA within a pH range of 2–10. Binding was independent of temperature between 36 and 42 °C. When kinetics of adsorption were evaluated, the Langmuir isotherm had a better fit (R 2 > 0.95) to adsorption equilibrium data than the Freundlich equation. The maximum amounts adsorbed (Q o) from the Langmuir model were 8.68 and 7.55 μM/g for MIP and NIP, respectively. Fourier transform infrared, scanning and tandem electron microscopy, and Brunauer–Emmett–Teller analysis confirmed a highly porous MIP structure with a greater surface area compared to NIP. Binding characteristics evaluated with computational strategy using molecular docking experiments and in vitro in a complex media (rumen fluid) indicated a stronger ETA adsorption by the tested composition selected among other polymeric materials and affinity of MIP compared with NIP. This study suggested the possible utility of MIP as a solid phase extraction sorbent for applications in analytical chemistry or sensing devices tailored to track ergot alkaloid incidence and the fate of those alkaloids in complex ruminal digestive samples.

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Calorimetric study of adsorption of human serum albumin onto silica powders

Cited by 10 publications

References 19 publications

Quasi-Spherical Cell Clusters Induced by a Polyelectrolyte Multilayer

Quasi-Spherical Cell Clusters Induced by a Polyelectrolyte Multilayer

Zwitteration: Coating Surfaces with Zwitterionic Functionality to Reduce Nonspecific Adsorption

Synthesis, Evaluation, and Characterization of an Ergotamine Imprinted Styrene-Based Polymer for Potential Use as an Ergot Alkaloid Selective Adsorbent

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