Calpeptin is neuroprotective against acrylamide-induced neuropathy in rats

Su, Benyu; Guan, Qiangdong; Wang, Miaomiao; Wei, Xiaomin; Wang, Shue; Yang, Xiwei; Jiang, Wenchong; Xu, Menglong; Sung, Yunsick

doi:10.1016/j.tox.2018.03.003

Cited by 16 publications

(17 citation statements)

References 37 publications

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“…In the hind limb splay examination [3,5], the hind paws of rats were inked, then the rats were placed in a horizontal position of 30 cm high and dropped onto a white paper. The distance between the center points of right and left heels were recorded as the landing foot spread distance.…”

Section: Behavioral Testsmentioning

confidence: 99%

“…In the gait score test [3,5], animals were placed on the table and were observed for 3 min. Gait was scored as follow: 1: normal gait; 2, slightly abnormal gait characterized by slight ataxia, weakness and foot splay; 3, moderately abnormal gait characterized by obvious ataxia and foot splay with limb spread during ambulation; 4, severely abnormal gait characterized by a combination of all the above symptoms, dragging hind limbs and inability to support body weight.…”

Section: Behavioral Testsmentioning

confidence: 99%

“…Accordingly, in recent years, some agents with antiapoptosis, antioxidant and anti-inflammatory properties have been expected to attenuate ACR-induced neurotoxicity [3,8,[11][12][13][14]. As the most active constituent in turmeric, a common spice, with a strong safety record, curcumin has been considered to be a potential natural neuroprotective agent under limelight [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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The anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin on acrylamide-induced neurotoxicity in rats

Guo

Cao

et al. 2020

BMC Pharmacol Toxicol

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Background: Acrylamide (ACR) formed during heating of tobacco and carbohydrate-rich food as well as widely applied in industries has been known as a well-established neurotoxic pollutant. Although the precise mechanism is unclear, enhanced apoptosis, oxidative stress and inflammation have been demonstrated to contribute to the ACRinduced neurotoxicity. In this study, we assessed the possible anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin, the most active component in a popular spice known as turmeric, on the neurotoxicity caused by ACR in rats. Methods: Curcumin at the dose of 50 and 100 mg/kg was orally given to ACR-intoxicated Sprague-Dawley rats exposed by ACR at 40 mg/kg for 4 weeks. All rats were subjected to behavioral analysis. The HE staining and terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL) staining were used to detect histopathological changes and apoptotic cells, respectively. The mRNA and protein expressions of apoptosis-related molecule telomerase reverse transcriptase (TERT) were detected using real-time PCR and immunohistochemistry, respectively. The contents of malondialdehyde (MDA) and glutathione (GSH) as well as the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured as the indicators for evaluating the level of oxidative stress in brain. The levels of pro-inflammatory cytokinestumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the cerebral homogenates were detected using ELISA assay. Results: ACR-induced weigh loss, deficits in motor function as well as pathological alterations in brains were significantly improved in rats administrated with 50 and 100 mg/kg curcumin. TUNEL-positive apoptotic cells in curcumin-treated ACR intoxicated brains were less than those in the ACR model group. Curcumin administration especially at the dose of 100 mg/kg upregulated the TERT mRNA expression and enhanced the number of TERTpositive cells in ACR-intoxicated cortex tissues. Moreover, curcumin treatment reduced the concentrations of TNF-α, IL-1β and MDA, while increased the GSH contents as well as the SOD and GSH-Px activities in the cerebral homogenates, in comparison to ACR control group.

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Section: Behavioral Testsmentioning

confidence: 99%

Section: Behavioral Testsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin on acrylamide-induced neurotoxicity in rats

Guo

Cao

et al. 2020

BMC Pharmacol Toxicol

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show abstract

“…As a chemical formed during the high-temperature processing of tobacco and carbohydrate-rich foods, acrylamide (ACR) is well recognized as a human neurotoxin which has posed signi cant public health concerns due to its daily intake [1][2][3]. Moreover, ACR is widely employed in various chemical and industrial processes as a component to produce polymers used in gel chromatography, dye synthesis, production of paper, cosmetics and waste water management, etc [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Accordingly, in recent years, some agents with anti-apoptosis, antioxidant and anti-in ammatory properties have been expected to attenuate ACR-induced neurotoxicity [3,8,[11][12][13][14]. As the most active constituent in turmeric, a common spice, with a strong safety record, curcumin has been considered to be a potential natural neuroprotective agent under limelight [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

The anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin on acrylamide-induced neurotoxicity in rats

Guo

Cao

et al. 2020

Preprint

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Background: Acrylamide (ACR) formed during heating of tobacco and carbohydrate-rich food as well as widely applied in industries has been known as a well-established neurotoxic pollutant. Although the precise mechanism is unclear, enhanced apoptosis, oxidative stress and inflammation have been demonstrated to contribute to the ACR-induced neurotoxicity. In this study, we assessed the possible anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin, the most active component in a popular spice known as turmeric, on the neurotoxicity caused by ACR in rats.Methods: Curcumin at the dose of 50 and 100 mg/kg was orally given to ACR- intoxicated Sprague-Dawley rats exposed by ACR at 40mg/kg for 4 weeks. All rats were subjected to behavioral analysis. The HE staining and terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL) staining were used to detect histopathological changes and apoptotic cells, respectively. The mRNA and protein expressions of apoptosis-related molecule telomerase reverse transcriptase (TERT) were detected using real-time PCR and immunohistochemistry, respectively. The contents of malondialdehyde (MDA) and glutathione (GSH) as well as the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured as the indicators for evaluating the level of oxidative stress in brain. The levels of pro-inflammatory cytokinestumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the cerebral homogenates were detected using ELISA assay.Results: ACR-induced weigh loss, deficits in motor function as well as pathological alterations in brains were significantly improved in rats administrated with 50 and 100 mg/kg curcumin. TUNEL-positive apoptotic cells in curcumin-treated ACR intoxicated brains were less than those in the ACR model group. Curcumin administration especially at the dose of 100 mg/kg upregulated the TERT mRNA expression and enhanced the number of TERT-positive cells in ACR-intoxicated cortex tissues. Moreover, curcumin treatment reduced the concentrations of TNF-α, IL-1β and MDA, while increased the GSH contents as well as the SOD and GSH-Px activities in the cerebral homogenates, in comparison to ACR control group.Conclusions: These data suggested the anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin on ACR-induced neurotoxicity in rats. Maintaining TERT-related anti-apoptotic function might be one mechanism underlying the protective effect of curcumin on ACR-intoxicated brains.

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High-fat diet exacerbated motor dysfunction via necroptosis and neuroinflammation in acrylamide-induced neurotoxicity in mice

Qiang,

Song,

Wang

et al. 2024

Ecotoxicology and Environmental Safety

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Calpeptin is neuroprotective against acrylamide-induced neuropathy in rats

Cited by 16 publications

References 37 publications

The anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin on acrylamide-induced neurotoxicity in rats

The anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin on acrylamide-induced neurotoxicity in rats

The anti-apoptotic, antioxidant and anti-inflammatory effects of curcumin on acrylamide-induced neurotoxicity in rats

High-fat diet exacerbated motor dysfunction via necroptosis and neuroinflammation in acrylamide-induced neurotoxicity in mice

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