Calprotectin Expression in Human Monocytes: Induction by <i>Porphyromonas gingivalis</i> Lipopolysaccharide, Tumor Necrosis Factor‐α, and Interleukin‐1β

Suryono, Suryono; Kido, Jun‐ichi; Hayashi, Noriko; Kataoka, Masatoshi; Nagata, Toshihiko

doi:10.1902/jop.2005.76.3.437

Cited by 55 publications

(26 citation statements)

References 37 publications

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“…Elevated GCF calprotectin levels in diseased groups might be due to the increased activation of inflammatory cells by bacterial components as well as greater release of intracellular material in gingivitis and periodontitis [45][46][47]. As a result, calprotectin could contribute to the host inflammatory immune defense against bacteria in periodontal disease [8,9,48,49]. In an in vitro study, epithelial calprotectin was shown to promote resistance to Porphyromonas gingivalis invasion and thereby reduce bacterial invasion [8].…”

Section: Discussionmentioning

confidence: 99%

Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

et al. 2011

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Abstract. Aim:The aim of the present study was to investigate gingival crevicular fluid (GCF) calprotectin, osteocalcin and cross-linked N-terminal telopeptide (NTx) levels in health along with different periodontal diseases. Material and methods: Twenty chronic periodontitis (CP), 20 generalized aggressive periodontitis (G-AgP), 20 gingivitis and 20 healthy subjects were included. Probing depth, clinical attachment level, plaque index and papillary bleeding index was recorded. GCF calprotectin, osteocalcin and NTx levels were analyzed by enzyme-linked immunosorbent assay (ELISA). Results: CP, G-AgP and gingivitis groups had higher GCF calprotectin total amount compared to healthy subjects (p < 0.008). CP and G-AgP groups had similar, but higher levels compared to gingivitis groups (p < 0.008). CP and G-AgP groups had lower GCF osteocalcin total amount compared to gingivitis and healthy groups (p < 0.008). CP group had higher GCF NTx but lower osteocalcin total amount and osteocalcin/NTx ratio than the G-AgP group (p < 0.008). Conclusions: Our results suggest that elevated GCF calprotectin levels play a role as a reliable inflammatory marker in the pathogenesis of periodontal disease. Fluctuating GCF levels of osteocalcin and NTx might point out to the abnormal bone turnover in periodontitis. Our data document for the first time the role of NTx in the pathogenesis of different periodontal diseases.

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Section: Discussionmentioning

confidence: 99%

Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

et al. 2011

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“…Calprotectin level in gingival crevicular fluid (GCF) of periodontitis patients was significanly higher than healthy subjects and it was detected in gingival tissue only from the periodontitis patients. 1,2 It was also known that calprotectin has the extracellular function, including antibacterial, chemotactic factor, and inhibition binding of pathogenic bacteria to the epithelial cell, suggest that calprotectin may play a role in the immune response mechanism of periodontitis 9 . However, the exact role of calprotectin in periodontitis patient with diabetes mellitus is unclear.…”

Section: Introductionmentioning

confidence: 99%

Expression of MRP8/MRP14 mRNA in Monocytes of Periodontitis: Comparison between Diabetic and Non Diabetic Patients

Syaify

Barunawati

Suryono

et al. 2010

The Indonesian J Dent Res

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The severity of periodontitis on patients with type 2 Diabetes Mellitus patients was strongly thought caused by decreasing of leukocytes function such as monoctyes and neutrophils. In our previous research it was found that calprotectin (MRP8/MRP14) level in leukocytes of periodontitis patients with type 2 DM was higher than periodontitis in non DM. The aim of this study was to determine calprotectin (MRP8/MRP14) mRNA expression in human monocytes of periodontitis patients with type 2 DM and without DM. Monocytes were isolated from the peripheral blood of periodontitis patients with uncontrolled type 2 DM, controlled type 2 DM, and non DM. The expression of total RNA calprotectin (MRP8 and MRP14) were detected by RT-PCR using GAPDH as the innate control. It was observed that the value of MRP8/MRP14 mRNA expression DM patients were higher than non DM, and the highly significant increase expression (p<0.05) was on the uncontrolled type 2 DM. The basal level of MRP8/MRP14 expression increased in monocyte of periodontitis and type 2 DM patients compared with non diabetes subjects. It was suggested that high basal level MRP8/ MRP14 has role in the regulation of severity periodontitis with diabetes mellitus.

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“…The concentration also increases compared with healthy patients. 8 In addition, the in vitro study shows that calprotectin has anti-microbe function, bacteriastatics and fungistatics with Minimal Inhibitory Concentration (MIC) that is similar with some antibiotics. The mechanism of this action is actually caused by the increasing of bacteria binding zinc needed for proliferation.…”

mentioning

confidence: 99%

“…The concentration of calprotectin in the gingival crevicular fluids can also reflect the severity of the inflammation in patients with periodontal disease. 7,8 Thus, in order to analyze the mechanism or pathogenesis of this disease, the concentration of calprotectin showing the degree of the inflammation in those patients with aggressive periodontitis must be examined.…”

mentioning

confidence: 99%

“…16 When calprotectin is discharged from neutrophile through the signal line of lipopolysaccaride, lipopolysaccaride interacts with some receptors, such as: CD14 and Toll-like receptor (TLR) that activate the function of nuclear factorkB (NF-kB), so it can cause the process of transcription in the nucleus and the process of translation in the ribosom, which then it is synthesized into calprotectin. 8,17 The concentration of calprotectin in gingival crevicular fluids increases about 12 times compared with that in chronic periodontitis patients and in the healthy patients in the longitudinal study of aggressive periodontitis patients. 16 Calprotectin is discharged through physiologic mechanism during the lifespan of neutrophile cells, in which there are more than 93.2% of cell viability after it is incubated with LPS P. gingivalis.…”

mentioning

confidence: 99%

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Detection of aggressive periodontitis by calprotectin expression

Sari

Suryono

2009

Dent. J. (Majalah Kedokteran Gigi)

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Calprotectin Expression in Human Monocytes: Induction by Porphyromonas gingivalis Lipopolysaccharide, Tumor Necrosis Factor‐α, and Interleukin‐1β

Abstract: These results demonstrate that P-LPS, TNF-alpha, and IL-1beta induce calprotectin production from human monocytes and that this production is associated with the activation of DNA C/EBPalpha binding complex.

Cited by 55 publications

References 37 publications

Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

Expression of MRP8/MRP14 mRNA in Monocytes of Periodontitis: Comparison between Diabetic and Non Diabetic Patients

Detection of aggressive periodontitis by calprotectin expression

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