Calprotectin (MRP8/14 protein complex) release during mycobacterial infection in vitro and in vivo

Pechkovsky, Dmitri V.; Zalutskaya, Oksana M.; Ivanov, G.I.; Misuno, Natalia I.

doi:10.1111/j.1574-695x.2000.tb01501.x

Cited by 54 publications

(20 citation statements)

References 25 publications

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“…6,18 Supporting this, Valheim et al 55 also found a high number of macrophages expressing Ki-67 in granulomas from subclinically paratuberculosis-affected goats, and high levels of calprotectin in plasma have been associated with severe and active tuberculosis in humans. 41 Opposed to this hypothesis, Jenkins et al 24 have pointed out that local macrophage proliferation is a signature of a Th2 inflammation. However, their study was conducted in pleural macrophages from rodents infected with a nematode, while Map is an intracellular pathogen affecting the intestine.…”

Section: Discussionmentioning

confidence: 97%

Macrophage Subsets Within Granulomatous Intestinal Lesions in Bovine Paratuberculosis

et al. 2016

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Animals infected with Mycobacterium avium subspecies paratuberculosis show a variety of granulomatous lesions that range from focal forms, seen in the subclinical stages, to diffuse lesions associated with clinical signs. The aim of this study was to phenotypically characterize the macrophages present in the different lesion types using immunohistochemical methods. Lesions from a total of 23 animals with bovine paratuberculosis, natural and experimental, were examined by immunohistochemistry. Antibodies against inducible nitric oxide synthase (iNOS), tumor necrosis factor α (TNF-α), CD163, interleukin 10 (IL-10), transforming growth factor β (TGF-β), major histocompatibility complex (MHC) class II, natural resistance-associated macrophage protein 1 (Nramp-1), calprotectin, Ki-67, CD68, lysozyme, and ionized calcium-binding adaptor molecule 1 (Iba-1) molecules were employed. Samples were scored semiquantitatively using a complete histological score (H-score), reflecting the staining intensity and the percentage of immunolabeled macrophages. Differences in the H-score were seen depending on the lesion type. In focal lesions, with none or few acid-fast bacilli (AFB), macrophages were polarized toward M1 phenotype, with high H-scores for iNOS and TNF-α. Diffuse multibacillary lesions showed M2 differentiation, with high expression of CD163, IL-10, and TGF-β as well as Nramp-1 and MHC class II antigens. Macrophages in diffuse paucibacillary forms showed high H-scores for iNOS but low ones for TNF-α. Diffuse lesions, either multibacillary or paucibacillary, showed high calprotectin and low Ki-67 expression, suggesting a progressive character, while focal forms, with low H-scores for these antigens, would be consistent with latency. Lysozyme and CD68 expression were related to the amount of AFB. H-score for Iba-1 antibody was similar among all types. The findings of this study provide insights into the polarization status of macrophages and lesion development in bovine paratuberculosis.

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Section: Discussionmentioning

confidence: 97%

Macrophage Subsets Within Granulomatous Intestinal Lesions in Bovine Paratuberculosis

et al. 2016

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“…The expression of S100A8 and S100A9 without concomitant formation of their complex has been rarely found, eg, in a chronic type of inflammatory reaction in glomerulonephritis or in chronic renal allograft rejection 76 77. Upregulation of the S100A8/9 complex, known as calprotectin, however, has been well described in inflammatory disorders such as cystic fibrosis,78-81 psoriasis,82 tuberculosis,83 Crohn disease and ulcerative colitis 66. The presence of S100A8 and S100A9 in infiltrated macrophages in RA was first detected in 1987 84.…”

Section: Proposed Function(s) Of Other S100 Proteins In Rheumatoid Armentioning

confidence: 99%

The metastasis associated protein S100A4: a potential novel link to inflammation and consequent aggressive behaviour of rheumatoid arthritis synovial fibroblasts

Oslejsková

Grigorian²,

Neidhart

et al. 2007

Annals of the Rheumatic Diseases

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The metastasis associated protein S100A4: a potential novel link to inflammation and consequent aggressive behaviour of rheumatoid arthritis synovial fibroblasts The metastasis associated protein S100A4: a potential novel link to inflammation and consequent aggressive behaviour of rheumatoid arthritis synovial fibroblasts AbstractThe metastasis-associated protein S100A4 belongs to the large family of S100 calcium-binding proteins that appear to play regulatory roles in diverse biological activities. Moreover, a prognostic role of S100A4 has been suggested for patients with several types of cancer. Cancer promoting properties for S100A4 have been demonstrated, particularly through its regulation of cell motility, proliferation and apoptosis, as well as by stimulation of angiogenesis and remodelling of the extracellular matrix. Increased expression of S100A4 mRNA has been detected in proliferating synovial fibroblasts in rheumatoid arthritis. Furthermore, strong upregulation of the S100A4 protein in rheumatoid arthritis synovial tissue compared with osteoarthritis and control tissues has been demonstrated recently, especially at sites of joint invasion. Several immune and vascular cells were also identified to be producing S100A4 within the synovium. The local upregulation of S100A4 was accompanied by high plasma and synovial fluid concentrations of the S100A4 protein existing in the bioactive oligomeric form in patients with rheumatoid arthritis. Consistent with data from cancer studies, the extracellular S100A4 oligomer appears to be involved in regulation of several matrix-degrading enzymes and modulation of the transcriptional activation function of the tumour suppressor protein p53 in rheumatoid arthritis synovial fibroblasts. Taken together, one can speculate that increased S100A4 protein in circulation and locally at sites of inflammation, particularly at sites of joint destruction, might be linked to the process of aggressive fibroblast behaviour contributing to the pathogenesis of chronic autoinflammatory diseases such as rheumatoid arthritis. The metastasis associated protein S100A4: a potential novel link to inflammation and consequent aggressive behavior of rheumatoid arthritis synovial fibroblasts AbstractThe metastasis-associated protein S100A4 belongs to the large family of calcium-binding proteins that appears to play regulatory roles in diverse biological activities. Moreover, a prognostic role of S100A4 has been suggested for patients with several types of cancer. Cancer promoting properties for S100A4 have been demonstrated, particularly through its regulation of cell motility, proliferation and apoptosis, as well as by stimulation of angiogenesis and remodeling of the extracellular matrix. Increased expression of S100A4 mRNA was detected in proliferating synovial fibroblasts in rheumatoid arthritis. Furthermore, strong up-regulation of S100A4 protein in rheumatoid arthritis synovial tissue compared with osteoarthritis and control tissues has been demonstrated recently, especially at the sites ...

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“…In lung infections, during the course of febrile conditions (26) or tuberculosis, (27) plasma levels of calprotectin were increased, as well as in acute organ rejection, (28) and rheumatoid arthritis. (29) Furthermore, calprotectin can be measured at inflamed tissue levels, e. g., in lung MØ contained in the bronchoalveolar lavage, (30) in renal MØ and epithelial cells, (31,4) in MØ and epithelial cells from skin lesions, (32) and in the synovial membrane in the joints.…”

Section: Introductionmentioning

confidence: 99%

“…(24) Proinflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, released by MØ, as well as arachidonic acid mediate the expression of calprotectin (20) that in turn enhances the expression of CD11b on human MØ, thus facilitating the mechanism of extravasation. (25) The derivation of calprotectin from inflammatory cells, such as PMN and MØ, has prompted many investigations on its detection in biological fluids in different clinical settings as a biomarker of inflammation.In lung infections, during the course of febrile conditions (26) or tuberculosis, (27) plasma levels of calprotectin were increased, as well as in acute organ rejection, (28) and rheumatoid arthritis. (29) Furthermore, calprotectin can be measured at inflamed tissue levels, e. g., in lung MØ contained in the bronchoalveolar lavage, (30) in renal MØ and epithelial cells, (31,4) in MØ and epithelial cells from skin lesions, (32) and in the synovial membrane in the joints.…”

mentioning

confidence: 99%

New Insights into the Biological and Clinical Significance of Fecal Calprotectin in Inflammatory Bowel Disease

Amati¹,

Passeri²,

Selicato³

et al. 2006

Immunopharmacology and Immunotoxicology

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Nowadays, calprotectin, a cytoplasmatic protein, released by activated neutrophilic polymorphonuclear cells (PMN) and/or monocytes-macrophages (MØ), is considered a good indicator of inflammation in several diseases. Accordingly, fecal calprotectin represents a good predictor of clinical relapse in ulcerative colitis (UC) patients, whereas conflicting results have been reported in Crohn's disease (CD) patients. In our study, in 76 IBD patients (29 CD and 47 UC) fecal calprotectin has been evaluated by a commercial ELISA kit. Results demonstrate that levels of this protein in the stool are significantly more elevated in active CD and UC patients than in normal volunteers. In quiescent CD and UC a trend to higher levels of calprotectin than in the normal counterpart is, however, evident. These data suggest that a low-grade inflammation of the intestinal wall is always present in CD and UC patients, which may predict a clinical relapse risk. In the same group of patients calprotectin levels also were analyzed according to sex and age. A trend to higher values of calprotectin was present in male patients with active or quiescent CD than in their female counterparts. Only in UC patients in remission a trend to calprotectin increase was more marked in the male group than in the female counterpart. When CD and UC patients were divided up according to age, calprotectin positivity peaked between 30-39 years in active CD patients, while in quiescent CD maximum positivity was between 40 and 49 years. However, in both active and quiescent UC patients, calprotectin positivity increased with age. The more precocious detectability of fecal calprotectin in CD patients, as a marker of intestinal mucosa inflammation, may be related to the different histopathology of the two diseases (CD versus UC). However, reduced PMN and/or MØ trafficking from peripheral blood to intestinal mucosa with age by effects of chronic treatment should not be ignored in CD patients.

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Calprotectin (MRP8/14 protein complex) release during mycobacterial infection in vitro and in vivo

Cited by 54 publications

References 25 publications

Macrophage Subsets Within Granulomatous Intestinal Lesions in Bovine Paratuberculosis

Macrophage Subsets Within Granulomatous Intestinal Lesions in Bovine Paratuberculosis

The metastasis associated protein S100A4: a potential novel link to inflammation and consequent aggressive behaviour of rheumatoid arthritis synovial fibroblasts

New Insights into the Biological and Clinical Significance of Fecal Calprotectin in Inflammatory Bowel Disease

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