Calreticulin enriched as an early-stage encapsulation protein in wax moth Galleria mellonella larvae

Choi, Jin-Ho; Whitten, Miranda M. A.; Cho, M.Y.; Lee, K.Y.; Kim, M.S.; Ratcliffe, Norman A.; Lee, B.L.

doi:10.1016/s0145-305x(01)00081-7

Cited by 49 publications

(40 citation statements)

References 16 publications

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“…Antibody and Immunochemistry-The generation and use of anti-Draper (14), anti-Croquemort (14), anti-focal adhesion kinase (21), anti-Pretaporter (17), and anti-Calreticulin (22,23) antibodies were reported previously. Anti-Drosophila DmCaBP1 antibody was raised by immunizing rats with recombinant DmCaBP1 expressed in E. coli as a protein fused to GST.…”

Section: Methodsmentioning

confidence: 99%

Apoptosis-dependent Externalization and Involvement in Apoptotic Cell Clearance of DmCaBP1, an Endoplasmic Reticulum Protein of Drosophila

Okada

Nagaosa

Kuraishi

et al. 2012

Journal of Biological Chemistry

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Background: Cells undergoing apoptosis are selectively recognized and engulfed by phagocytes. Results: A Drosophila endoplasmic reticulum protein named DmCaBP1 was externalized upon apoptosis, bound to both apoptotic cells and phagocytes, and enhanced phagocytosis. Conclusion: DmCaBP1 connects apoptotic cells and phagocytes to promote phagocytosis. Significance: We are first to report that a protein residing in the endoplasmic reticulum plays a role in apoptotic cell clearance as a tethering molecule.

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Section: Methodsmentioning

confidence: 99%

Apoptosis-dependent Externalization and Involvement in Apoptotic Cell Clearance of DmCaBP1, an Endoplasmic Reticulum Protein of Drosophila

Okada

Nagaosa

Kuraishi

et al. 2012

Journal of Biological Chemistry

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“…A proleg was amputated, and hemolymph was allowed to drip into an ice-cold, sterile Eppendorf tube containing 1.5 ml of decoagulation buffer (Deco; pH 5.5) prepared as described previously (32) and saturated with phenylthiourea (PTU) to inhibit the prophenoloxidase system. Hemocytes were washed twice by centrifugation at 200 ϫ g for 2 min at 4°C and resuspended as described for each assay below.…”

Section: Insect Rearing and Bleedingmentioning

confidence: 99%

“…These include the highly conserved multifunctional Ca 2ϩ transport protein, calreticulin (32); an 86-kDa protein with sequence homology to insect diapause protein 1 (33); as well as a novel 56-kDa protein (34). The role of calreticulin in mammalian immunity has only recently been reported, but it appears to be important for cell adhesion, Ag presentation, phagocytosis, and inflammation (35).…”

mentioning

confidence: 99%

A Novel Role for an Insect Apolipoprotein (Apolipophorin III) in β-1,3-Glucan Pattern Recognition and Cellular Encapsulation Reactions

Whitten

Tew

Lee

et al. 2004

The Journal of Immunology

205

163

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Lipoproteins and molecules for pattern recognition are centrally important in the innate immune response of both vertebrates and invertebrates. Mammalian apolipoproteins such as apolipoprotein E (apoE) are involved in LPS detoxification, phagocytosis, and possibly pattern recognition. The multifunctional insect protein, apolipophorin III (apoLp-III), is homologous to apoE. In this study we describe novel roles for apoLp-III in pattern recognition and multicellular encapsulation reactions in the innate immune response, which may be of direct relevance to mammalian systems. It is known that apoLp-III stimulates antimicrobial peptide production in insect blood, enhances phagocytosis by insect blood cells (hemocytes), and binds and detoxifies LPS and lipoteichoic acid. In the present study we show that apoLp-III from the greater wax moth, Galleria mellonella, also binds to fungal conidia and β-1,3-glucan and therefore may act as a pattern recognition molecule for multiple microbial and parasitic invaders. This protein also stimulates increases in cellular encapsulation of nonself particles by the blood cells and exerts shorter term, time-dependent, modulatory effects on cell attachment and spreading. All these responses are dose dependent, occur within physiological levels, and, with the notable exception of β-glucan binding, are only observed with the lipid-associated form of apoLp-III. Preliminary studies also established a beneficial role for apoLp-III in the in vivo response to an entomopathogenic fungus. These data suggest a wide range of immune functions for a multiple specificity pattern recognition molecule and may provide a useful model for identifying further potential roles for homologous proteins in mammalian immunology, particularly in terms of fungal infections, pneumoconiosis, and granulomatous reactions.

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“…The possible reason might be formation of a nodule requires that circulating haemocytes change from non-adhesive to adhesive cells that are able to bind to the target and one another and this change requires the involvement of plasma (Clark et al, 1997;Choi et al, 2002;Nardi et al, 2005;Shu et al, 2016). Some molecules have been reported to be involved in this process, such as extracellular matrix (ECM) proteins lacunin and the ligand for peanut agglutinin (PNA) lectin in M. sexta (Nardi et al, 2005), calrecticulin in Galleria melonella (Choi et al, 2002), and plasmatocyte spreading peptide (PSP) in the soybean looper moth, Pseudoplusia includens Walker (Lepidoptera: Noctuidae) (Clark et al, 1997) etc. Therefore, we hypothesize that Asian corn borer plasma supplies similar molecules for the nodule formation upon the challenge of B. bassiana conidia.…”

Section: Discussionmentioning

confidence: 99%

Cellular immune response of the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Pyralidae), to infection by the entomopathogenic fungus, Beauveria bassiana

Shen¹,

Li²,

Cheng³

et al. 2016

Eur. J. Entomol.

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Abstract. The term cellular immune response refers to haemocyte-mediated responses, including phagocytosis, nodulation, and encapsulation. In the present study, we identifi ed fi ve types of circulating haemocytes in larvae of the haemolymph of the Asian corn borer, Ostrinia furnacalis (Guenée), including granulocytes, oenocytoids, plasmatocytes, prohaemocytes, and spherulocytes. The relative number of total free haemocytes per larva decreased signifi cantly 0.5, 24, and 36 h after the injection of Beauveria bassiana conidia. Upon conidia challenge, both phagocytosis and nodulation were observed in the collected haemolymph from O. furnacalis larvae. In addition, plasma was found to be necessary for both phagocytosis and nodulation. Therefore, we here confi rm that phagocytosis and nodulation are involved in O. funacalis larvae during their fi ght against infection by B. bassiana, and further, that the cellular immune response of O. furnacalis helps eliminate the invading organisms despite the fact that not all the fungal conidia are killed.

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Calreticulin enriched as an early-stage encapsulation protein in wax moth Galleria mellonella larvae

Cited by 49 publications

References 16 publications

Apoptosis-dependent Externalization and Involvement in Apoptotic Cell Clearance of DmCaBP1, an Endoplasmic Reticulum Protein of Drosophila

Apoptosis-dependent Externalization and Involvement in Apoptotic Cell Clearance of DmCaBP1, an Endoplasmic Reticulum Protein of Drosophila

A Novel Role for an Insect Apolipoprotein (Apolipophorin III) in β-1,3-Glucan Pattern Recognition and Cellular Encapsulation Reactions

Cellular immune response of the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Pyralidae), to infection by the entomopathogenic fungus, Beauveria bassiana

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