Calumenin and fibulin-1 on tumor metastasis: Implications for pharmacology

Zheng, Pengli; Wang, Qiao; Jiang, Teng; Chen, Jianguo

doi:10.1016/j.phrs.2015.05.001

Cited by 19 publications

(16 citation statements)

References 83 publications

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“…Calumenin is thought to have a role in inhibiting tumor metastasis, and its expression levels have been reported to be altered in cancer of the head and neck, endometrium, liver, pancreas, lung, and colon. 53 CALU expression was demonstrated to be increased in the tumoral stroma (cancerassociated fibroblasts) and epithelium of colorectal cancer samples. 54 Activating mutations in CTNNB1 have been associated with desmoid fibromatosis, 55 indicating a role in fibroblastic tumors.…”

Section: Discussionmentioning

confidence: 95%

USP6 activation in nodular fasciitis by promoter-swapping gene fusions

et al. 2017

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Nodular fasciitis is a self-limited myofibroblastic lesion that can be misdiagnosed as a sarcoma as a result of its rapid growth, cellularity, and sometimes prominent mitotic activity. A recurrent translocation t(17;22) has been identified in nodular fasciitis, fusing the coding region of USP6 to the promoter region of MYH9, and resulting in increased USP6 expression. A subset of cases show USP6 rearrangement without the typical fusion variants by RT-PCR, or any MYH9 rearrangement by FISH. We sought to further characterize such tumors using molecular diagnostic assays. A novel RT-PCR assay was designed to detect the two known MYH9-USP6 fusion types in formalin-fixed paraffin-embedded and frozen tissue, and a break-apart FISH assay was designed to detect USP6 rearrangement. Twenty-six cases of nodular fasciitis diagnosed between 2002 and 2013 were retrieved from the pathology files of our institutions and were confirmed to be positive by FISH and/or RT-PCR. Seven samples showed USP6 rearrangement by FISH but were negative for MYH9-USP6 fusion by RT-PCR; these cases were subjected to a next-generation sequencing assay utilizing anchored multiplex PCR technology. This assay targets a single partner gene associated with fusions in bone and soft tissue tumors for agnostic detection of gene fusion partners. Novel fusion partners were identified in all seven cases and confirmed by RT-PCR. Structurally, all fusions consisted of the juxtaposition of the entire coding region of USP6 with the promoter of the partner gene, driving increased USP6 expression. This study confirms the neoplastic nature of nodular fasciitis, defines additional pathogenic fusion partners, and adds to the growing body of literature on USP6-associated neoplasia. Given the diagnostic challenges of these tumors, molecular assays can be useful ancillary tools; however, the prevalence of promoter swapping must be recognized when interpreting results.

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Section: Discussionmentioning

confidence: 95%

USP6 activation in nodular fasciitis by promoter-swapping gene fusions

et al. 2017

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“…Why does the expression of Djcalu need to be strictly regulated by DjPiwiB‐piRNA complexes? In other animals, calumenin is a multifunctional Ca 2+ ‐binding protein involved in ER function, cell migration, and other functions (Zheng, Wang, Teng, & Chen, ). In addition, in the mouse brain, neural progenitors specifically express Calu , which might have roles in the migration and differentiation of neurons (Vasiljevic et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…One possibility is that these genes have been accidentally duplicated in the D. japonica genome, and dosage compensation is therefore required. (Zheng, Wang, Teng, & Chen, 2015). In addition, in the mouse brain, neural progenitors specifically express Calu, which might have roles in the migration and differentiation of neurons (Vasiljevic et al, 2012).…”

Section: Djpiwib-pirna Complexes Coordinate Proper Gene Expression mentioning

confidence: 99%

Searching for non‐transposable targets of planarian nuclear PIWI in pluripotent stem cells and differentiated cells

2018

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Nuclear PIWIs together with their guide RNAs (piRNAs) epigenetically silence various genes including transposons in many organisms. In planarians, the nuclear piwi family gene, DjpiwiB is specifically transcribed in adult pluripotent stem cells (adult PSC, neoblast), but not in differentiated cells. However, the protein accumulates in the nuclei of both neoblasts and their descendant differentiated cells. Interestingly, PIWI(DjPiwiB)-piRNA complexes are indispensable for the repression of transposable genes at the onset of differentiation from neoblasts. Here, we conducted a comparative transcriptome analysis between control and DjpiwiB(RNAi) animals to identify non-transposable target genes of the DjPiwiB-piRNA complexes. Using bioinformatic analyses and RNAi we demonstrate that DjPiwiB-piRNA complexes are required for the proper expression of Djmcm2 and Djhistone h4 in neoblasts and that DjPiwiB-piRNA complexes regulate the transient expression of Djcalu during neoblast differentiation. Thus, DjPiwiB-piRNA complexes regulate the correct expression patterns during neoblast self-renewal and differentiation.

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“…Calumenin (CALU) is a calcium‐binding protein that belongs to the CREC family and contains six EF‐hand helix–loop–helix motifs with a low affinity for Ca2 + as well as an endoplasmic reticulum retention signal (Honoré, ; Honoré & Vorum, ). The CREC family proteins show various subcellular localization including cytosol, nucleus, Golgi apparatus, endoplasmic reticulum, extracellular space, as well as cell membrane, and participate in a large variety of functions such as calcium homoeostasis, secretory cargo sorting, chaperone activity, cell migration and cell proliferation (Zheng, Wang, Teng, & Chen, ). Recently, 15 isoforms of CALU, calumenin‐1 to ‐15, were identified in human cells (Feng et al., ).…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of different developmental stages of swamp buffalo testicular seminiferous tubules

Zhang

et al. 2017

Reprod Domestic Animals

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With ageing, many protein components change markedly during mammalian spermatogenesis. Most of these proteins have yet to be characterized and verified. Here, we have employed two-dimensional electrophoresis coupled to tandem mass spectrometry to explore the different proteins from pre-pubertal, pubertal and post-pubertal swamp buffalo testicular seminiferous tubules. The results showed that 25 protein spots were differentially expressed among developmental stages, and 13 of them were successfully identified by mass spectrometry. Of which four proteins were up-regulated and three proteins were down-regulated with age, and the remaining six proteins were fluctuated among developmental stages. Bioinformatics analysis indicates that these proteins were probably related to cellular developmental process (53.8%), cell differentiation (53.8%), spermatogenesis (15.4%), apoptotic process and cell death (30.8%). Expression profiles of calumenin (CALU) and galectin-1 (LGALS1) were further verified via Western blotting. In summary, the results help to develop an understanding of molecular mechanisms associated with buffalo spermatogenesis.

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Calumenin and fibulin-1 on tumor metastasis: Implications for pharmacology

Cited by 19 publications

References 83 publications

USP6 activation in nodular fasciitis by promoter-swapping gene fusions

USP6 activation in nodular fasciitis by promoter-swapping gene fusions

Searching for non‐transposable targets of planarian nuclear PIWI in pluripotent stem cells and differentiated cells

Comparative proteomic analysis of different developmental stages of swamp buffalo testicular seminiferous tubules

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