To better understand the optimization of the stator profile to improve the dynamic performance of a novel profiled chamber metering pump, this paper presents a dynamic analysis method for transition curve design and optimization of the profiled chamber. The face-shaped curve of the inner chamber of the stator is formed of two quarter circular arcs and two quarter noncircular arcs, and the two quarter noncircular arcs are defined as transition curves, which directly affect the mechanical vibration, friction, and wear of the profiled chamber metering pump. The proposed design and optimization method combine a friction analysis with the motion control of the moving parts. The approach based on a new strategy mainly includes three steps. For the first step, based on motion analysis, the trigonometric function and polynomial function are adopted to derive the candidate transition curves that have different order of continuity. Secondly, a friction model between the slides and the stator is established, by means of kinematic and force analysis of the slides. Then, the model is used to examine the friction dissipated energy for different candidate transition curves. Finally, through a comparison analysis of motion parameters and energy losses, comprehensive optimal transition curves are obtained. This three-step analytical work is proved to be efficient in the design of the transition curves.