Camel's Milk Protects Against Aluminum Chloride-Induced Normocytic Normocromic Anemia, Lipid Peroxidation and Oxidative Stress in Erythrocytes of White Albino Rats

Abstract

<b>Problem statement:</b> Aluminum (Al) is an indifferent element from a toxicological point of view. In recent years, however, Al has been implicated in the pathogenesis of several clinical disorders. One of the most frequently described problem in aluminum toxicity is anemia. The present study was carried out to determine the effectiveness of Camel’s milk in alleviating the toxicity of aluminum chloride (AlCl₃) on certain hematological parameters, lipid peroxidation and oxidative stress enzyme in the RBC’s of white albino rats. <b>Approach:</b> Ten rats per group were divided into three treatment groups: Group one were rats given normal saline and served as control group, group two were rats treated with 1 ml of AlCl₃ (0.5 mg kg^-1 body weight) and named AlCl₃ treated rats, group 3 were rat treated with 1ml fresh camel’s milk 10 min before the administration of AlCl₃ (0.5 mg kg^-1 body weight) and named Camel’s milk and AlCl₃ treated rats. Rats were orally administered their respective doses every day for 30 days. Evaluations were made for hematological parameters in the blood and for lipid peroxidation and oxidative stress enzymes activities in the RBC’s. <b>Results:</b> Results obtained showed that oral AlCl₃ treatment caused a significant decrease (p<0.05) in total erythrocytes count, blood Hemoglobin (Hb), hematocrite (PCV) and Serum iron levels, where as the values of Mean Corpuscular Volume (MCV), Mean Hemoglobin Concentration (MHC), Mean Corpuscular Hemoglobin Concentration (MCHC) and Total Ion Binding Capacity (TIBC) didn’t change. Also oral administration of AlCl₃ induced free radicals and as a result caused an increase the concentration of Thiobarbituric Acid Reactive Substances (TBARS) and decreased activities of Superoxide Dismutase (SOD) and Catalsae (CAT) in the RBCs homolysate. The oral administration of Camel’s milk before the administration of AlCl₃, alleviated it’s toxic effect. Camel’s milk administration resulted in a significant increase (p<0.05) in the in total erythrocytes count, blood hemoglobin (Hb), hematocrite (PCV) and Serum iron with No change in the values of MCV, MHC, MCHC and TIBC when compared to AlCl₃ treated rats. Camel’s milk reduced free radicals production and oxidative stress status in the RBC’s noticed by the significant decreased levels of TBARS and increased activities of SOD and CAT when compared to AlCl₃ treated rats. <b>Conclusion:</b> our data proved that there is an alternation in the hematological parameters and antioxidant system in the red blood cells of rats administered aluminum chloride orally, whereas oral administration of Camel’s milk prior the administration of Aluminum chloride protects the red blood cell form toxic effect of aluminum

Camel Milk Beneficial Effects on Treating Gentamicin Induced Alterations in Rats

“…GM toxicity enhances oxidative stress and the so formed reactive oxygen species (ROS) that exhaust the countering antioxidant enzymes and biomolecules [ 27 – 30 ], referring to further impaired pathway processes [ 31 ]. The significant increase in the LDH of GM injected rats compared to controls is also in agreement with previously published findings [ 3 , 13 ]. It was seen that the MDA (lipid peroxidation marker) and inflammatory marker MPO activity were significantly increased, whilst the endogenous antioxidants GST and SOD were decreased.…”

“…It was seen that the MDA (lipid peroxidation marker) and inflammatory marker MPO activity were significantly increased, whilst the endogenous antioxidants GST and SOD were decreased. This finding is consistent with researchers' reports that attribute the inactivation of enzymes to their cross-linking with MDA that lead to increased accumulation of superoxide free radicals inducing more lipid peroxidation [ 13 , 16 , 28 , 30 , 32 , 33 ].…”

Protective effects of synbiotic diets of Bacillus coagulans, Lactobacillus plantarum and inulin against acute cadmium toxicity in rats

“…We realized that the synbiotic diets resulted in increasing the SOD enzyme against cadmium toxicity. AL-Hashem [ 28 ] reported significant reduction in SOD activity of rats that were exposed to aluminium chloride. Similar assumption was proposed by Tian et al [ 17 ].…”

Inhibitory potentials of Cymbopogon citratus oil against aluminium-induced behavioral deficits and neuropathology in rats

“…Transferrin is known primarily for its role in the transport and cellular uptake of iron but is also the major serum binding protein for aluminum [37]. One of the mechanisms through which Al exerts its neurotoxicity is its ability to hinder iron transport and absorption by displacing iron from binding to transferrin and therefore inhibit iron metabolism [38]. We investigated cerebellar levels of TRP following the oral infusion of Al and ESO in Wistar rats.…”