Camelid Single-Domain Antibodies for the Development of Potent Diagnosis Platforms

Silva, Nairo Brilhante da; Sousa, Rosa Maria de Oliveira; Arruda, Andrelisse; Santos, Eliza Lima Dos; Marinho, Anna Carolina Machado; Stábeli, Rodrigo G.; Fernandes, Carla Freire Celedônio; Pereira, Soraya dos Santos

doi:10.1007/s40291-021-00533-7

Cited by 8 publications

(3 citation statements)

References 153 publications

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“…Another diagnostic method that can be used is blood sampling for serology using the lateral-flow rapid test (RT) or multiantigen print immunoassay (MAPIA). The lack of light chain in camelid antibodies, which makes forming immune complexes difficult, limits the sensitivity of lateral-flow assays [ 42 , 47 ]. A study carried out by Lyashchenko et al [ 48 ] on a limited number of animals showed that serological tests, such as MAPIA and RT, allowed the ante-mortem correct determination of infection status ( M. microti ) in 97 and 87% of the animals tested, respectively.…”

Section: Disease Caused By Mycobacterium Tuberculosis ...mentioning

confidence: 99%

A Literature Review of Selected Bacterial Diseases in Alpacas and Llamas—Epidemiology, Clinical Signs and Diagnostics

Konieczny,

Pomorska-Mól

2023

Animals

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The breeding of South American Camelids (SACs), particularly llamas and alpacas, is becoming increasingly popular in regions that are not their natural habitat, including Europe. These animals are considered to be relatively disease resistant. However, due to their growing popularity, special attention should be given to infections in llamas and alpacas. Knowledge of bacterial infections is very important to veterinarians and breeders. Many of these diseases also have zoonotic potential, so these animals must be considered as sources of potential zoonotic infections. Due to the limited information on many diseases occurring in llamas and alpacas, veterinarians often rely on data collected in other animal species, focusing on cattle, sheep and horses. This work aims to summarise the knowledge of diseases caused by Clostridium spp., Mycobacterium tuberculosis complex, Mycobacterium avium subsp. paratuberculosis, Streptococcus spp., Escherichia coli, Pasteurella multocida, Manheimia haemolytica and Corynebacterium pseudotuberculosis in llamas and alpacas, with particular attention to epidemiology, clinical signs and diagnostics.

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Section: Disease Caused By Mycobacterium Tuberculosis ...mentioning

confidence: 99%

A Literature Review of Selected Bacterial Diseases in Alpacas and Llamas—Epidemiology, Clinical Signs and Diagnostics

Konieczny,

Pomorska-Mól

2023

Animals

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“…Conventional antibodies have proven to be robust workhorses in various cell biology research fields 1 . Notwithstanding their indispensability, their large size and complex architecture preclude them from functioning within living cells 2 .…”

Section: Introductionmentioning

confidence: 99%

Development of a multifunctional toolkit of intrabody-based biosensors recognizing the V5 peptide tag: highlighting applications with G protein-coupled receptors

Zeghal

Matte

Venes

et al. 2023

Preprint

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Protein-protein interactions (PPIs) form the underpinnings of any cellular signaling network. PPIs are highly dynamic processes and often, cell-based assays can be essential for their study as they closely mimic the biological intricacies of cellular environments. Since no sole platform can perform all needed experiments to gain a thoroughly comprehensive understanding into these processes, developing a versatile toolkit is much needed to address this longstanding gap. The use of small peptide tags, such as the V5-tag, has been extensively used in biological and biomedical research, including labeling the C-termini of one of the largest human genome-wide open-reading frame collections. However, these small peptide tags have been primarily used in vitro and lack the in vivo traceability and functionality of larger specialized tags. In this study, we combined structural studies and computer-aided maturation to generate an intracellular nanobody, interacting with the V5-tag. Suitable for assays commonly used to study protein-protein interactions, our nanobody has been applied herein to interrogate G protein-coupled receptor signalling. This novel serviceable intrabody is the cornerstone of a multipurpose intracellular nanobody-based biosensors toolkit, named iBodyV5, which will be available for the scientific community at large.

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“…Given the indispensability of these peptides, antibodies have continually been developed to recognize existing and newly developed epitope tags. Although conventional antibodies have proven to be robust workhorses in various cell biology research fields, their large size and complex architecture preclude them from functioning within living cells ( 14 , 15 ). These limitations spurred advances in engineering novel recombinant antibody constructs, termed intracellular antibodies or intrabodies.…”

mentioning

confidence: 99%

Development of a V5-tag–directed nanobody and its implementation as an intracellular biosensor of GPCR signaling

Zeghal

Matte

Venes

et al. 2023

Journal of Biological Chemistry

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Camelid Single-Domain Antibodies for the Development of Potent Diagnosis Platforms

Cited by 8 publications

References 153 publications

A Literature Review of Selected Bacterial Diseases in Alpacas and Llamas—Epidemiology, Clinical Signs and Diagnostics

A Literature Review of Selected Bacterial Diseases in Alpacas and Llamas—Epidemiology, Clinical Signs and Diagnostics

Development of a multifunctional toolkit of intrabody-based biosensors recognizing the V5 peptide tag: highlighting applications with G protein-coupled receptors

Development of a V5-tag–directed nanobody and its implementation as an intracellular biosensor of GPCR signaling

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