In the process of the deformation monitoring for large-scale structure, the mobile vision method is often used. However, most of the existent researches rarely consider the real-time property and the variation of the intrinsic parameters. This paper proposes a real-time deformation monitoring method for the large-scale structure based on a relay camera. First, we achieve the real-time pose-position relationship by using the relay camera and the coded mark points whose coordinates are known. The real-time extrinsic parameters of the measuring camera are then solved according to the constraint relationship between the relay camera and the measuring camera. Second, the real-time intrinsic parameters of the measuring camera are calculated based on the real-time constraint relationship among the extrinsic parameters, the intrinsic parameters, and the fundamental matrix. Finally, the coordinates of the noncoded measured mark points, which are affixed to the surface of the structure, are achieved. Experimental results show that the accuracy of the proposed method is higher than 1.8 mm. Besides, the proposed method also possesses the real-time and automation property.