Campesin, a thermostable antifungal peptide with highly potent antipathogenic activities

Lin, Peng; Wong, Jack Ho; Xia, Lixin; Ng, Tzi Bun

doi:10.1016/j.jbiosc.2009.03.013

Cited by 14 publications

(13 citation statements)

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“…The characteristics of the isolated peptide were then compared with those of its previously reported counterparts from other Brassica species. 3 N-Terminal Amino Acid Sequence Analysis. The N-terminal amino acid sequence of the purified protein was performed by Edman degradation using a Hewlett-Packard amino acid sequencer.…”

Section: ' Introductionmentioning

confidence: 99%

“…Antifungal proteins and peptides are produced by a diversity of organisms ranging from bacteria to flowering plants to mammals . The antifungal proteins and peptides reported to date exhibit a variety of amino acid sequences and molecular sizes. ,− …”

Section: Introductionmentioning

confidence: 99%

“…Biological activities other than antifungal activity, including antiproliferative activity toward tumor cells and inhibitory activity toward HIV-1 reverse transcriptase, have been reported for these proteins. − ,,− ,, …”

Section: Introductionmentioning

confidence: 99%

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Protein from Red Cabbage (Brassica oleracea) Seeds with Antifungal, Antibacterial, and Anticancer Activities

et al. 2011

J. Agric. Food Chem.

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A 30 kDa antifungal protein was purified from red cabbage ( Brassica oleracea ) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange chromatography on Q-Sepharose and SP-Sepharose followed by fast protein liquid chromatography on Mono S. The protein hindered mycelial growth in Mycosphaerella arachidicola (with an IC50=5 μM), Setospaeria turcica, and Bipolaris maydis. It also inhibited the yeast Candida albicans with an IC50=96 μM. It exerted its antifungal action by permeabilizing the fungal membrane as evidenced by staining with Sytox green. The antifungal activity was stable from pH 3 to 11 and from 0 to 65 °C. It manifested antibacterial activity against Pseudomonas aeruginosa (IC50=53 μM). Furthermore, after 48 h of culture, it suppressed proliferation of nasopharyngeal cancer and hepatoma cells with IC50=50 and 90 μM, respectively.

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Section: ' Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protein from Red Cabbage (Brassica oleracea) Seeds with Antifungal, Antibacterial, and Anticancer Activities

et al. 2011

J. Agric. Food Chem.

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“…Table 3 lists the antifungal proteins and peptides previously isolated from Brassica spp. The molecular weights of juncin, brassiparin and kale peptides were 18.9 kDa, 5716 Da, and 5907 Da [30,31,32], respectively, while the molecular weight of BGAP was 8.5 kDa, which was closer to those of campesin (9.4 kDa) and nsLTP (9414 Da) [33,34]. However, both campesin and nsLTP were similar in sequence to lipid transfer protein, while BGAP was structurally similar to defensin, indicating that BGAP is a peptide different from the abovementioned peptides.…”

Section: Discussionmentioning

confidence: 99%

Purification of an Antifungal Peptide from Seeds of Brassica oleracea var. gongylodes and Investigation of Its Antifungal Activity and Mechanism of Action

Wang

Zhang

et al. 2019

Molecules

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In this study, a 8.5-kDa antifungal peptide designated as BGAP was purified from the crude extract of the seeds of Brassica oleracea var. gongylodes by employing a protocol that comprised cation exchange chromatography on SP-Sepharose, cation exchange chromatography on Mono S and gel filtration chromatography on Superdex peptide. BGAP showed the highest amino acid sequence similarity to defensin peptides by mass spectrometric analysis. BGAP showed a broad spectrum of antifungal activity with a half maximal inhibitory concentration at 17.33 μg/mL, 12.37 μg/mL, 16.81 μg/mL, and 5.60 μg/mL toward Colletotrichum higginsianum, Exserohilum turcicum, Magnaporthe oryzae and Mycosphaerella arachidicola, respectively. The antifungal activity of BGAP remained stable (i) after heat treatment at 40–100 °C for 15 min; (ii) after exposure to solutions of pH 1–3 and 11–13 for 15 min; (iii) after incubation with solutions containing K+, Ca2+, Mg2+, Mn2+ or Fe3+ ions at the concentrations of 20–150 mmol/L for 2 h; and (iv) following treatment with 10% methyl alcohol, 10% ethanol, 10% isopropanol or 10% chloroform for 2 h. Fluorescence staining experiments showed that BGAP brought about an increase in cell membrane permeability, a rise in reactive oxygen species production, a decrease in mitochondrial membrane potential, and an accumulation of chitin at the hyphal tips of Mycosphaerella arachidicola.

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“…A wide range of plants and animals produce antifungal compounds such as proteins, peptides, (Vasilevskii et al, 2007) and fungi (Wang and Ng, 2004). In plants, various tissues, including, fruits, bulbs, leaves, seeds, roots and tubers synthesize antifungal proteins and peptides (Lin et al, 2009). The present work is carried out to evaluate the antifungal potential of methanolic extracts of selected medicinal plants (Azadirachta indica, Alstonia scholaris) against Sclerotium rolfsii.…”

Section: Introductionmentioning

confidence: 99%

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2016

J. Bioresource Manage.

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Sclerotium rolfsii is a polyphagous soil borne fungus infecting more than 500 plant species across the world that is causing vast losses. Although the fungus is soil and seed borne, soil borne inoculum is most vital in infection-causing and syndrome development. Treating soil borne pathogens with fungicides is not reasonable due to very high costs. Environmental hazards are also involved. Therefore, integrated management of pathogens using biological controlling agents is the paramount alternative. Extracts of higher plants have demonstrated a wide range of activity against plant pathogenic organisms. The present research work was carried out to manage the pathogen and disease in vitro by using plant extracts. The antifungal activity of the methanolic leaf extract of two medicinally important indigenous plants, Alstonia scholaris and Azadirachta indica, against the fungal pathogen S. rolfsii was evaluated. In vitro antifungal bioassay was conducted against the S. rolfsii using different concentrations (0, 1, 2 and 5) of the methanolic leaf extract of A. scholaris and A. indica using malt extract (ME) broth as a culture medium. Different concentrations of A. indica leaf extract appreciably reduced the fungal biomass growth up to 76% as compared to the control. In the same manner, various concentrations of the leaf extract of A. scholaris significantly decreased fungal biomass up to 70% as compared to the control. Higher fungal growth was reduced by a 2% concentration of both plants. The present study concludes that the methanolic extract of A. indica has more active antifungal components and can be effectively used to manage phyto-pathogens.

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Campesin, a thermostable antifungal peptide with highly potent antipathogenic activities

Cited by 14 publications

References 56 publications

Protein from Red Cabbage (Brassica oleracea) Seeds with Antifungal, Antibacterial, and Anticancer Activities

Protein from Red Cabbage (Brassica oleracea) Seeds with Antifungal, Antibacterial, and Anticancer Activities

Purification of an Antifungal Peptide from Seeds of Brassica oleracea var. gongylodes and Investigation of Its Antifungal Activity and Mechanism of Action

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