Camphene isolated from essential oil of Piper cernuum (Piperaceae) induces intrinsic apoptosis in melanoma cells and displays antitumor activity in vivo

Girola, Natália; Figueiredo, Carlos R.; Farias, Camyla F.; Azevedo, Ricardo A.; Ferreira, Adilson Kleber; Teixeira, Sarah Fernandes; Capello, Tabata M.; Martins, Euder Glendes Andrade; Matsuo, Alisson L.; Travassos, Luiz R.; Lago, João Henrique G.

doi:10.1016/j.bbrc.2015.10.041

Cited by 101 publications

(78 citation statements)

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“…The main compounds of EOs such as α-humulene, β-caryophyllene, myrcene and β-pinene exhibited popularbioactivities against fungal infectious diseases, viruses and tumors [13,14]. Camphene isolated from Piper cernuum EO exerted antitumor activity against melanoma cells [15]. Limonene and β-elemene can be regarded as broad-spectrum antitumor agents [16][17][18].…”

Section: Discussionmentioning

confidence: 99%

The Essential Oils from <em>Pittosporum tobira</em> Flowers Display Antiproliferative Properties and Inducing Apoptosis on Lung Carcinoma Cells

Sun¹,

Wang²,

Lü³

et al. 2017

Preprint

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Abstract:Background: Pittosporum tobira (Thunb.) Aiton is an aromatic medicinal plant widely cultivated in the world. However, the essential oils (EOs) from P. tobira flowers for anti-cancer potential is still not systematically studied. The present aim to elucidate the phytochemical composition of the EOs and to explore mechanism of anticancer action. Methods: The EOs were extracted and analyzed by headspace solid-phase microextraction (HS-SPME) with gas chromatography-mass spectrometry (GC-MS). Volatile components were identified according to Kovats retention index (KI) and NIST database. The anti-neoplasm mechanisms of the EOs was comprehensively investigated in lung carcinoma A549 and H460 cells. Results: A total of 47 secondary metabolites representing 94.18% of the EOs were successfully identified: monoterpenes and sesquiterpenes were the dominant terpenoids. The EOs exhibited antiproliferative activity on A549 and H460 lung carcinoma cells.Hoechst 33324 fluorescent staining indicated the typical characteristics of apoptosis and induced cycle phase arrest. AnnexinV/PI staining revealed that the number of apoptotic cells was increased.Furthermore, the EOs also induced the caspase-mediated mitochondrial apoptosis pathway. Conclusions:Findings suggest that the full-scale chemical composition and first characterization of anticancer activities of the EOs, it could be used for integrative natural anti-cancer agents in health care should be pay more attention.

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Section: Discussionmentioning

confidence: 99%

The Essential Oils from <em>Pittosporum tobira</em> Flowers Display Antiproliferative Properties and Inducing Apoptosis on Lung Carcinoma Cells

Sun¹,

Wang²,

Lü³

et al. 2017

Preprint

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“…Many Piper species are biologically active and have shown antitumor [21], antimicrobial [22], antioxidant, insecticide, larvicide [5], anti-inlammatory, antinociceptive, enzyme inhibitor, trypanocidal, antiplatelet, piscicide, allelopathic, antiophidic, anti-malaria, antileishmania, ansiliotico/antidepressant, antituberculosis, antidiabetic, nematocide, herbicide, hepatoprotective, anti-Helicobacter pylori [23], amebicide [24] and diuretic [25] potential.…”

Section: Piper Genusmentioning

confidence: 99%

“…In a study conducted by Girola et al [21], the monoterpene camphene isolated from essential oil of P. cernuum, collected in Cubatão in the state of São Paulo, induced apoptosis in melanoma cells, and showed antitumor activity in vivo, thus showing itself to be a promising compound in cancer therapy.…”

Section: Biological Activities Of Essential Oils Of the Piper Speciesmentioning

confidence: 99%

Biological Properties of Essential Oils from the Piper Species of Brazil: A Review

Takeara¹,

Gonçalves²,

SantosAyres³

et al. 2017

Aromatic and Medicinal Plants - Back to Nature

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Piperaceae, a Latin name derived from Greek, which in turn originates from the Arabic word babary-black pepper, is considered one of the largest families of basal dicots, found in tropical and subtropical regions of both hemispheres. The species that belong to this family have a primarily pantropical distribution, predominantly herbaceous members, occurring in tropical Africa, tropical Asia, Central America and the Amazon region. The Piperaceae family includes ive genera: Piper, Peperomia, Manekia, Zippelia and Verhuellia.Brazil has about 500 species distributed in the Piper, Peperomia and Manekia genera. The Piper genus, the largest of the Piperaceae family, has about 4000 species. Within the Piper genus, about 260-450 species can be found in Brazil. Piper species have diverse biological activities and are used in pharmacopeia throughout the world. They are also used in folk medicine for treatment of many diseases in several countries including Brazil, China, India, Jamaica and Mexico. Pharmacological studies of Piper species point toward the vast potential of these plants to treat various diseases. Many of these species are biologically active and have shown antitumor, antimicrobial, antioxidant, insecticidal, antiinlammatory, antinociceptive, enzyme inhibitor, antiparasitic, antiplatelet, piscicide, allelopathic, antiophidic, anxiolytic, antidepressant, antidiabetic, hepatoprotective, amebicide and diuretic possibilities.

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“…It biosynthesizes polyphenols as flavonoids and acid-phenols (Girola et al, 2015;Ćetković, Djilas, Cannadanović-Brunet & Tubas, 2004), which possess pharmacological properties against cancer (Cabrera & Mach, 2012;Girola et al, 2015), a multifactorial disease that has been related with oxidative stress and inflammatory processes (Lobo, Patil, Phatak & Chandra, 2010). This suggests its use first as a bio functional food due to its high content of phenolic compounds with antioxidant capacity, and perhaps as the base of a new treatments.…”

Section: +mentioning

confidence: 99%

“…Previous studies have demonstrated that plant phenols act as free-radical scavengers, reduce Fe +3 /Fe +2 , chelate metals, quench singlet and triplet oxygen, inhibit peroxidation and have an anti-inflammatory effect by inhibiting pro-inflammatory cytokine expression (Farahpour, 2014;Girola et al, 2015).…”

Section: +mentioning

confidence: 99%

Perfil de polifenoles, capacidad antioxidante y efecto citotóxico in vitro en líneas celulares humanas de un extracto hidroalcohólico de pétalos de Calendula officinalis L.

et al. 2018

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ARTÍCULO ORIGINAL abstractThe hydro-alcoholic extract from Calendula officinalis petals, was evaluated showing beneficial effects against oxidative stress and therefore on diseases associated to inflammatory processes. The content of polyphenols of the extract was determined by TLC and HPLC-UV-DAD and the antioxidant efficiency estimated from Fe 3+ /Fe 2+ reducing power, Cu 2+and Fe 2+ chelation,• OH radical scavenging, ABTS [2, 2´-azino bis (3 ethylbenzothiazoline-6-sulphonic acid)] and DPPH (2,2´diphenyl-1-picrylhydrazyl) assays. The in vitro growth inhibition (IC 50 ) in human cancer cell breast (MDA-MB-231), uterine cervix (HeLa), lung (A-549), colon (HT-29) and (Caco-2) was determined by MTT method. The extract showed that the polyphenols content in the hydro-alcoholic extract was 37.01±0.015 mg GAE g -1 , being rutin the most abundant one. ABTS TEAC and DPPH TEAC tests showed a high antioxidant capacity. The antioxidant activity profile displayed high reducing capacity and ability to chelate Cu 2+ and Fe 2+ as well as good• OH radical scavenging capacity. The extract showed a cytotoxic effect on human cancer cell lines of colon (HT-29) and (Caco-2), breast (MDA-MB-231), uterine cervix (HeLa), and lung (A-549) (IC 50 = 10.84, 11.73, 11.26, 11.53, and 11.96 µg mL -1 , respectively). The correlation analysis suggests that the Calendula officinalis polyphenols are directly related to the antioxidant efficiency of the extract and inversely to the cell viability.Key Words: Calendula officinalis, polyphenols, antioxidant, cytotoxicity. OH, y ensayos ABTS [2, 2´-azino-bis (3 etilbenzotiazolin-6-sulfónico) y DPPH (2,2´difenil-1-picrilhidracilo). La inhibición del crecimiento in vitro (IC 50 ) en células cancerosas humanas de mama (MDA-MB-231), de cuello uterino (HeLa) , pulmón (A-549) , y colon (HT-29 y Caco-2), fue determinado por el método de MTT. El extracto mostró que el contenido total de polifenoles fue de 37.01±0.015 mg GAe g -1 , siendo la rutina la más abundante. Las pruebas ABTS TEAC y DPPH TEAC mostraron una alta capacidad antioxidante. El perfil de actividad antioxidante mostró buena capacidad reductora y capacidad para quelar Cu 2+ y Fe 2+, así como una buena capacidad de eliminación de radicales • OH. El extracto también mostró efecto citotóxico en células de colon (HT-29 y Caco-2) , de mama (MDA-MB-231), de cuello uterino (HeLa) y pulmón (A-549) (IC 50 = 10.84, 11.73, 11.26, 11.53 y 11.96 μg mL -1 , respectivamente). El análisis de correlación sugiere que los polifenoles de Calendula officinalis están directamente relacionados con la capacidad antioxidante del extracto y se asocian inversamente con la viabilidad celular.Palabras Clave: Calendula officinalis, polifenoles, antioxidante, citotoxicidad.

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Camphene isolated from essential oil of Piper cernuum (Piperaceae) induces intrinsic apoptosis in melanoma cells and displays antitumor activity in vivo

Cited by 101 publications

References 28 publications

The Essential Oils from <em>Pittosporum tobira</em> Flowers Display Antiproliferative Properties and Inducing Apoptosis on Lung Carcinoma Cells

The Essential Oils from <em>Pittosporum tobira</em> Flowers Display Antiproliferative Properties and Inducing Apoptosis on Lung Carcinoma Cells

Biological Properties of Essential Oils from the Piper Species of Brazil: A Review

Perfil de polifenoles, capacidad antioxidante y efecto citotóxico in vitro en líneas celulares humanas de un extracto hidroalcohólico de pétalos de Calendula officinalis L.

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