Campylobacter Enteritis in Brussels

Lauwers, Sabine; Boeck, M. De; Butzler, Jean‐Paul

doi:10.1016/s0140-6736(78)91045-0

Cited by 107 publications

(36 citation statements)

References 4 publications

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“…jejuni were used in this study. They were isolated from human stools in the laboratory of the Sint Pierre Hospital in 1976 and 1977 by means of the selective coproculture technique described by Butzler et al (3), simplified by omitting the preliminary filtration of the stools (10 ,ug/ml did not inhibit any of them. Penicillin G…”

Section: Methodsmentioning

confidence: 99%

Susceptibility of Campylobacter fetus subsp. jejuni to Twenty-Nine Antimicrobial Agents

Vanhoof

Vanderlinden

Dierickx

et al. 1978

Antimicrob Agents Chemother

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128

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The activity of 29 antimicrobial agents was tested against 95 strains of Campylobacter fetus subsp. jejuni isolated from human stools. Furazolidone and gentamycin were the most active agents. The tetracyclines, erythromycin, and chloramphenicol were very active against most of the strains, but with each antibiotic a few resistant strains were found. The penicillins were relatively inactive, and the cephalosporins tested were only active against occasional strains.The aims of this study were to determine the general susceptibility pattern of Campylobacter fetus subsp.jejuni; to recognize those antimicrobial agents to which it is generally susceptible and which might therefore be useful in the treatment of enteritis or septicemia due to Campylobacter; and to identify those antimicrobial agents to which it is always resistant and which might be used to develop an improved selective medium.In an early study of susceptibilities, Butzler et al. (4) showed that most strains of Campylobacter (Vibrio) fetus were resistant to the penicillins and almost all to cephalothin. We have now determined the in vitro activity of 29 antimicrobial agents against 95 strains isolated from patients' stools. MATERIALS AND METHODSBacterial strains. Ninety-five strains of C. fetus subsp. jejuni were used in this study. They were isolated from human stools in the laboratory of the Sint Pierre Hospital in 1976 and 1977 by means of the selective coproculture technique described by Butzler et al. (3), simplified by omitting the preliminary filtration of the stools (10). Strains were preserved as suspensions in fluid thioglycolate agar medium (Difco no. 0256-01) and stored in liquid nitrogen. When required, the suspensions were thawed and inoculated onto petri dishes containing fluid thioglycolate agar medium (Difco no. 0256-01) and stored in liquid nitrogen. When required, the suspensions were thawed and inoculated onto petri dishes containing fluid thioglycolate agar medium (Difco no. 0256-01) to which had been added 0.075% agar and 10% defibrinated sheep blood and, per milliliter, 25 IU of bacitracin (Nutritional Biochemicals Corp., Cleveland, Ohio), 0.005 mg of novobiocin (The Upjohn Co., Kalamazoo, Mich.), and 0.05 mg of actidione (Upjohn). The inoculated plates were incubated at 370C for at least 3 days in ordinary air with 10% CO2 added, since it had been found that subcultures of these organisms did not require any further reduction of the oxygen tension for growth. The carbon dioxide level was maintained by an automatic C02 controller (Portamatic, Forma Scientific Inc.). The colonies from the plates were then suspended in fluid thioglycolate medium and incubated for 24 h in 10% C02. Before the overnight cultures were used for inoculum, their turbidity was adjusted to match a McFarland no. 2 standard.Antibiotic susceptibility tests. The replica-inoculating apparatus described by Steers et al. (15) was used to apply the organisms to the surface of plates containing twofold dilutions of the antimicrobial agents. The medium used for sus...

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Section: Methodsmentioning

confidence: 99%

Susceptibility of Campylobacter fetus subsp. jejuni to Twenty-Nine Antimicrobial Agents

Vanhoof

Vanderlinden

Dierickx

et al. 1978

Antimicrob Agents Chemother

Self Cite

128

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“…A total of 493 samples from patients with enteritis were subjected to enrichment in Brain Heart Infusion (BHI) at 37°C and cell lysates subjected to PCR. The same samples were also cultured directly onto modified Butzler medium (Lauwers et al, 1978;Patton et al, 1981). The primers used, CF1 and CR2 (Table 6), amplified an 843-bp sequence of the 16S rRNA gene of all species of Campylobacter.…”

Section: Restriction Fragment Length Polymorphism Analysis (Pcr-rflp)mentioning

confidence: 99%

Campylobacter jejuni: A Review of its Characteristics, Pathogenicity, Ecology, Distribution, Subspecies Characterization and Molecular Methods of Detection

Levin

2007

Food Biotechnology

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Campylobacter jejuni is considered to be the leading cause of enteric illness in the United States and other industrialized nations, causing mild to severe symptoms including serious infections of the extremities and permanent neurological symptoms. The organism is a cytochrome oxidase positive, microaerophilic, curved Gram-negative rod exhibiting corkscrew motility and is carried in the intestine of many wild and domestic animals, particularly avian species including poultry, where the intestine is colonized resulting in healthy animals as carriers. Wildlife have long been considered an infectious reservoir for campylobacters because of their close association with and contamination of surface waters. This review deals with the areas of: phenotypic characteristics of C. jejuni and related human pathogenic species of Campylobacter, their ecological distribution, virulence factors, isolation of C. jejuni from foods, serotyping of Campylobacter isolates, bacteriophage typing, molecular methods of detecting and typing campylobacters, the viable but nonculturable state of campylobacters, the coccoid form of C. jejuni and immunomagnetic capture of C. jejuni.

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“…However, in situations where direct plating before enrichment is desirable, several selective agar media are available. Park et al, (1984) recommended that 2 to 3 loops full of cell concentrate collected by surface rinse or swab sampling techniques be transferred to the following selective agar media: Skirrow's agar (Skirrow, 1977) or Campy-BAP medium (Blaser et al, 1979;Kaplan, 1980), and Butzler's (Lauwers et al, 1978), modified Butzler's (Patton et al, 1981), or Preston (Bolton and Robertson, 1982) agars. The media are then streaked to obtain isolated colonies.…”

Section: Procedures For Detection and Enumerationmentioning

confidence: 99%

Methods for Detecting and Enumerating Campylobacter jejuni and Campylobacter coli in Poultry

Beuchat

1986

Poultry Science

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Methods, media, and biochemical tests for detecting, enumerating, isolating, and identifying Campylobacter jejuni and C. coli in foods are summarized with special consideration of poultry and poultry products. Information is drawn largely from the American Public Health Association Compendium of Methods for the Microbiological Examination of Foods and the US Food and Drug Administration Bacteriological Analytical Manual for Foods. Reference is also made to recently advanced techniques and procedures.

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Campylobacter Enteritis in Brussels

Cited by 107 publications

References 4 publications

Susceptibility of Campylobacter fetus subsp. jejuni to Twenty-Nine Antimicrobial Agents

Susceptibility of Campylobacter fetus subsp. jejuni to Twenty-Nine Antimicrobial Agents

Campylobacter jejuni: A Review of its Characteristics, Pathogenicity, Ecology, Distribution, Subspecies Characterization and Molecular Methods of Detection

Methods for Detecting and Enumerating Campylobacter jejuni and Campylobacter coli in Poultry

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