Campylobacter jejuni adhere to and invade chicken intestinal epithelial cells in vitro

Byrne, Catherine; Clyne, Marguerite; Bourke, Billy

doi:10.1099/mic.0.2006/000711-0

Cited by 128 publications

(120 citation statements)

References 51 publications

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“…Our results corroborate the occurrence of bacterium internalization in cultured primary chicken cells, as previously described (Byrne, Clyne, & Bourke, 2007), and may suggest that chicken ileum explants could be an alternative method to investigate C. jejuni invasiveness. Curiously, although mucin proteins in chicken intestines reduces C. jejuni internalization by the host cell (Alemka et al, 2010), that phenomenon was apparently not impaired in the C. jejuni-inoculated chicken ileum explants.…”

Section: Campylobacter Jejuni Increases Transcribed Il-1β and Causes supporting

confidence: 79%

Campylobacter Jejuni Increases Transcribed Il-1 B and Causes Morphometric Changes in the Ileal Enterocytes of Chickens

Fonseca

Júnior

Santos

et al. 2016

Rev. Bras. Cienc. Avic.

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Section: Campylobacter Jejuni Increases Transcribed Il-1β and Causes supporting

confidence: 79%

Campylobacter Jejuni Increases Transcribed Il-1 B and Causes Morphometric Changes in the Ileal Enterocytes of Chickens

Fonseca

Júnior

Santos

et al. 2016

Rev. Bras. Cienc. Avic.

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“…jejuni is driven by energy taxis rather than chemotaxis. Environmental taxis navigation mediated via CheA and CheY is essential for C. jejuni colonization and interaction with epithelial cells (9,44,51). However, this taxis navigation is most likely not mediated through transmembrane MCP-like proteins in a classical and metabolism-independent chemotaxis response, since the MCP-like proteins of this study did not mediate a detectable chemotaxis response.…”

Section: Resultsmentioning

confidence: 62%

“…It is speculated that the attraction toward chicken mucus directs and retains C. jejuni in the optimal environment of the avian intestinal lumen and thus prevents direct interaction with epithelial cells. This notion is based on in vitro observations where chicken mucus inhibited C. jejuni invasion of primary human epithelial cells, while increased invasion was observed for mutants carrying deletions of either cheA or cheY (9,44,51).…”

mentioning

confidence: 99%

Energy Taxis Drives Campylobacter jejuni toward the Most Favorable Conditions for Growth

Vegge

Brøndsted

et al. 2009

Appl Environ Microbiol

121

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Campylobacter jejuni is a serious food-borne bacterial pathogen in the developed world. Poultry is a major reservoir, and C. jejuni appears highly adapted to the gastrointestinal tract of birds. Several factors are important for chicken colonization and virulence, including a taxis mechanism for environmental navigation. To explore the mechanism of chemotaxis in C. jejuni, we constructed mutants with deletions of five putative mcp (methyl-accepting chemotaxis protein) genes (tlp1, tlp2, tlp3, docB, and docC). Surprisingly, the deletions did not affect the chemotactic behavior of the mutants compared to that of the parental strain. However, the tlp1, tlp3, docB, and docC mutant strains displayed a 10-fold decrease in the ability to invade human epithelial and chicken embryo cells, hence demonstrating that the corresponding proteins affect the host interaction. LAsparagine, formate, D-lactate, and chicken mucus were identified as new attractants of C. jejuni, and we observed that chemical substances promoting tactic attraction are all known to support the growth of this organism. The attractants could be categorized as carbon sources and electron donors and acceptors, and we furthermore observed a correlation between an attractant's potency and its efficiency as an energy source. The tactic attraction was inhibited by the respiratory inhibitors HQNO (2-n-heptyl-4-hydroxyquinoline N-oxide) and sodium azide, which significantly reduce energy production by oxidative phosphorylation. These findings strongly indicate that energy taxis is the primary force in environmental navigation by C. jejuni and that this mechanism drives the organism toward the optimal chemical conditions for energy generation and colonization.

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“…Those results support previous in vivo observation of the preventive effect of the heat-killed forms of both strains in pigs (Bernardeau & Guilmoto, 2008). In this study, we demonstrate that coaggregation properties of those two probiotic strains are not species specific since coaggregation effects were registered towards and extra-intestinal sequelae including bacteraemia and Guillain-Barré syndrome (Byrne et al, 2007). C. jejuni is sensitive to lactic acid (van Netten et al, 1995;Byrd et al, 2001;Dibner & Buttin, 2002).…”

Section: Discussionmentioning

confidence: 89%

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

113

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Bacterial aggregation and/or adhesion are key factors for colonization of the digestive ecosystem and the ability of probiotic strains to exclude pathogens. In the present study, two probiotic strains, Lactobacillus rhamnosus CNCM-I-3698 and Lactobacillus farciminis CNCM-I-3699, were evaluated as viable or heat-killed forms and compared with probiotic reference Lactobacillus strains (Lb. rhamnosus GG and Lb. farciminis CIP 103136). The autoaggregation potential of both forms was higher than that of reference strains and twice that of pathogenic strains. The coaggregation potential of these two beneficial micro-organisms was evaluated against several pathogenic agents that threaten the global safety of the feed/food chain: Escherichia coli, Salmonella spp., Campylobacter spp. and Listeria monocytogenes. The strongest coaggregative interactions were demonstrated with Campylobacter spp. by a coaggregation test, confirmed by electron microscopic examination for the two forms. Viable forms were investigated for the nature of the bacterial cell-surface molecules involved, by sugar reversal tests and chemical and enzymic pretreatments. The results suggest that the coaggregation between both probiotic strains and C. jejuni CIP 70.2 T is mediated by a carbohydrate-lectin interaction. The autoaggregation potential of the two probiotics decreased upon exposure to proteinase, SDS or LiCl, showing that proteinaceous components on the surface of the two lactobacilli play an important role in this interaction. Adhesion abilities of both Lactobacillus strains were also demonstrated at significant levels on Caco-2 cells, mucin and extracellular matrix material. Both viable and heat-killed forms of the two probiotic lactobacilli inhibited the attachment of C. jejuni CIP 70.2 T to mucin. In conclusion, in vitro assays showed that Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, as viable or heat-killed forms, are adherent to different intestinal matrix models and are highly aggregative in vitro with pathogens, especially Campylobacter spp., the most commonly reported zoonotic agent in the European Union. This study supports the need for further in vivo investigations to demonstrate the potential food safety benefits of Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, live or heat-killed, in the global feed/food chain.

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Campylobacter jejuni adhere to and invade chicken intestinal epithelial cells in vitro

Cited by 128 publications

References 51 publications

Campylobacter Jejuni Increases Transcribed Il-1 B and Causes Morphometric Changes in the Ileal Enterocytes of Chickens

Campylobacter Jejuni Increases Transcribed Il-1 B and Causes Morphometric Changes in the Ileal Enterocytes of Chickens

Energy Taxis Drives Campylobacter jejuni toward the Most Favorable Conditions for Growth

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

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