Campylobacter jejuni loss of culturability in aqueous microcosms and ability to resuscitate in a mouse model

Baffone, Wally; Casaroli, A.; Citterio, Barbara; Pierfelici, Lucia; Campana, Raffaella; Vittoria, Emanuela; Guaglianone, Emilio; Donelli, Gianfranco

doi:10.1016/j.ijfoodmicro.2005.08.015

Cited by 111 publications

(98 citation statements)

References 38 publications

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“…induction of re-culturable state) of Campylobacter cells are not understood. It has been reported that the in vivo resuscitation occurred by inoculation of non-culturable C. jejuni to 1-day chicks or embryonated eggs [4,11]. Jones et al [25] demonstrated that a suckling mouse passage of non-culturable cells induced intestinal bacterial colonization.…”

Section: Discussionmentioning

confidence: 99%

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Physiological Characterization of Campylobacter jejuni under Cold Stresses Conditions: Its Potential for Public Threat

et al. 2012

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ABSTRACT. Campylobacter jejuni is the major cause of human gastroenteritis worldwide. Under stress conditions, C. jejuni can enter a viable but non-culturable (VBNC) state. We found that the C. jejuni was able to enter a VBNC state by prolonged incubation at 4C. The standard isolation methods using pre-enrichment steps in Bolton broth or Preston broth could not detect the VBNC cells in spiked chicken meat. The transcription levels of virulence-associated genes (flaA, flaB, cadF, ciaB, cdtA, cdtB and cdtC) were expressed in VBNC cells but in low levels. The VBNC cells retained the ability to invade Caco-2 human intestinal epithelial cells in vitro. In most cases, VBNC cells failed to resuscitate in Caco-2 cells, but in some experiments, they formed colonies after co-incubation with host cells. Collectively, C. jejuni enters into a VBNC state at 4C and the VBNC C. jejuni remains virulent which may possibly lead to disease in humans. C. jejuni in VBNC state is a potential concern for food safety.KEY WORDS: Campylobacter jejuni, resuscitation, viable but nonculturable state, virulence.

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Section: Discussionmentioning

confidence: 99%

“…microcosm water) at 37°C. C. jejuni has been described to enter into the VBNC state in adverse environments such as pH stress, and combination of starvation and low temperature [4,11,14,25,32,45]. However, it was uncertain whether VBNC C. jejuni retains virulency and become culturable in human cells.…”

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confidence: 99%

Physiological Characterization of Campylobacter jejuni under Cold Stresses Conditions: Its Potential for Public Threat

et al. 2012

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“…But frequently observed and most vigorously discussed, Campylobacter transforms into a coccoid form, which definitely fails to grow in vitro [5]. However, such bacterial suspensions are capable of infection in various animal models, from which spiral and culturable Campylobacter were reisolated [6,7]. Also, the invasion of human epithelial cells was shown using coccoids without capacity to grow on agar plates [8].…”

Section: Detection Limitations Of Campylobacter and The Impact Of Viamentioning

confidence: 99%

Quo vadis? — MonitoringCampylobacterin Germany

Stingl

Knüver

Vogt

et al. 2012

European Journal of Microbiology and Immunology

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“…The enrichment procedure increases the recovery of damaged cells present in water (19) and could also increase the growth of any background microflora present, particularly in fecally contaminated water (1,15), that could prevent the detection of Campylobacter. Moreover, the presence of viable but noncultivable (VBNC) forms, under stressful environmental conditions (2,8,10), may also produce a negative result. Moreover, cells initially present in water can be irreversibly damaged if sampling or storage is not appropriate, and these can also not be detected.…”

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confidence: 99%

Development of a Rapid and Sensitive Method Combining a Cellulose Ester Microfilter and a Real-Time Quantitative PCR Assay To Detect Campylobacter jejuni and Campylobacter coli in 20 Liters of Drinking Water or Low-Turbidity Waters

Tissier¹,

Denis

Hartemann³

et al. 2012

Appl Environ Microbiol

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Investigations of Campylobacter jejuni and Campylobacter coli in samples of drinking water suspected of being at the origin of an outbreak very often lead to negative results. One of the reasons for this failure is the small volume of water typically used for detecting these pathogens (10 to 1,000 ml). The efficiencies of three microfilters and different elution procedures were determined using real-time quantitative PCR to propose a procedure allowing detection of Campylobacter in 20 liters of drinking water or low-turbidity water samples. The results showed that more than 80% of the bacteria inoculated in 1 liter of drinking water were retained on each microfilter. An elution with a solution containing 3% beef extract, 0.05 M glycine at pH 9, combined with direct extraction of the bacterial genomes retained on the cellulose ester microfilter, allowed recovery of 87.3% (؎22% [standard deviation]) of Campylobacter per 1 liter of tap water. Recoveries obtained from 20-liter volumes of tap water spiked with a C. coli strain were 69.5% (؎10.3%) and 78.5% (؎15.1%) for 91 CFU and 36 CFU, respectively. Finally, tests performed on eight samples of 20 liters of groundwater collected from an alluvial well used for the production of drinking water revealed the presence of C. jejuni and C. coli genomes, whereas no bacteria were detected with the normative culture method in volumes ranging from 10 to 1,000 ml. In the absence of available epidemiological data and information on bacterial viability, these last results indicate only that the water resource is not protected from contamination by Campylobacter.

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Campylobacter jejuni loss of culturability in aqueous microcosms and ability to resuscitate in a mouse model

Cited by 111 publications

References 38 publications

Physiological Characterization of Campylobacter jejuni under Cold Stresses Conditions: Its Potential for Public Threat

Physiological Characterization of Campylobacter jejuni under Cold Stresses Conditions: Its Potential for Public Threat

Quo vadis? — MonitoringCampylobacterin Germany

Development of a Rapid and Sensitive Method Combining a Cellulose Ester Microfilter and a Real-Time Quantitative PCR Assay To Detect Campylobacter jejuni and Campylobacter coli in 20 Liters of Drinking Water or Low-Turbidity Waters

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