Campylobacter jejuni strains isolated from chicken meat harbour several virulence factors and represent a potential risk to humans

Melo, Roberta Torres de; Nalevaiko, Priscila Christen; Mendonça, Eliane Pereira; Borges, Leandro Willian; Fonseca, Belchiolina Beatriz; Beletti, Marcelo Emı́lio; Rossi, D. A.

doi:10.1016/j.foodcont.2013.02.032

Cited by 38 publications

(33 citation statements)

References 24 publications

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“…Furthermore, this gene had a high prevalence in Campylobacter isolates in human cases and chicken carcasses [15]. In the present study, this gene in C. coli was more prevalent in isolates from chickens than those from pigs.…”

Section: Discussionsupporting

confidence: 49%

Macrolide resistance mechanisms and virulence factors in erythromycin-resistant <i>Campylobacter</i> species isolated from chicken and swine feces and carcasses

Lim

Moon

Chae

et al. 2016

The Journal of Veterinary Medical Science

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Resistance to antimicrobials was measured in 73 isolates of Campylobacter jejuni (C. jejuni) and 121 isolates of Campylobacter coli (C. coli) from chicken and swine feces and carcasses in Korea. Both bacterial species showed the highest resistance to (fluoro) quinolones (ciprofloxacin and nalidixic acid) out of the nine antimicrobials tested. Erythromycin resistance was much higher in C. coli (19.0%, 23/121) than in C. jejuni (6.8%, 5/73). The mutation in the 23S rRNA gene was primarily responsible for macrolide resistance in Campylobacter isolates. Several amino acid substitutions in the L4 and L22 ribosomal proteins may play a role in the mechanism of resistance, but the role requires further evaluation. A total of eight virulence genes were detected in 28 erythromycin-resistant Campylobacter isolates. All C. jejuni isolates carried more than four such genes, while C. coli isolates carried fewer than three such genes. The high rate of resistance highlights the need to employ more prudent use of critically important antimicrobials, such as fluoroquinolones and macrolides, in swine and poultry production, and to more carefully monitor antimicrobial resistance in Campylobacter isolates in food animals.

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Section: Discussionsupporting

confidence: 49%

Macrolide resistance mechanisms and virulence factors in erythromycin-resistant <i>Campylobacter</i> species isolated from chicken and swine feces and carcasses

Lim

Moon

Chae

et al. 2016

The Journal of Veterinary Medical Science

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“…These recesses provide ideal conditions for bacteria to adhere to and support the variations in processing conditions (JANG et al, 2007). Therefore, it is able to survive in frozen meat (-20ºC) even after long periods, which makes this type of food a potential source of infection in case they are not properly cooked in the right temperature (70-75ºC for 5 minutes) before consumption (MELO, 2013).…”

Section: Campylobacter In Broiler Carcassesmentioning

confidence: 99%

“…The pldA gene is related to cellular invasion and encodes a protein involved in the synthesis of outer membrane phospholipase (ZIPRIN et al, 2001). Melo et al (2013) analyzed virulence genes in C. jejuni strains isolated from poultry carcasses in Brazil showing that this species has great importance in the pathogenesis of human disease. This work showed that flaA, pldA, cadA, ciaB and cdtABC genes involved in adhesion, colonization, invasion of cells and toxin production process, were present in 74.5%, 63.6%, 67.3%, 67.3% and 65.5% of the strains, respectively.…”

Section: Pathogenesis and Virulencementioning

confidence: 99%

“…The ability of invasion seems to be strain dependent and strains isolated from the environment are much less invasive in HeLa cells than strains isolated from humans or animals (EVEREST et al, 1993). Melo et al (2013) selected five strains classified as most virulent to verify its ability to alter the morphology of Caco-2 cells and observed a loss of cells confluence for all strains tested. This loss of cellular integrity, reflected by loss of the ability to transport fluids and electrolytes between cells, contributes to the clinical symptoms of campylobacteriosis in vivo.…”

Section: Pathogenesis and Virulencementioning

confidence: 99%

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Campylobacteriosis: an emerging zoonosis, underdiagnosed and underreported by public health agencies in Brazil

et al. 2015

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ABSTRACT:The infection by Campylobacter spp. constitutes a zoonosis of worldwide distribution, with serious repercussions for public health and a significant socio-economic impact. The Campylobacter species that most often are implicated in outbreaks of gastrointestinal disease are C. jejuni and C. coli. Both are recognized as the most important and with greater pathogenic potential from the viewpoint of food safety. This review aims to clarify and discuss, based on literature, the epidemiology of Campylobacter spp. and the main challenges in their control, to assist in better understanding of the risk posed to human health. Due to the difficulty of isolating and cultivating of Campylobacter spp., the high cost and not mandatory, laboratory diagnosis by traditional cultivation techniques is not widely used in Brazil, resulting in lack of accurate data on the occurrence of the disease in the country. This factor has been identified as responsible for underdiagnosing and underreporting of the disease, which means that data on contamination of animal products or outbreaks are not statements of actual national situation. The epidemiology of Campylobacter spp. is considered complex presenting multiple determinants in the spread of this microorganism. Chickens are important reservoirs of Campylobacter sp. and the main target of control measures, however, other vehicles of transmission, such as raw cow milk, drinking water and other foods of animal origin must also be considered in the set of measures to be taken to control disease. The infection by Campylobacter spp. cause gastroenteritis in humans and occurred by oral contact with infected animals. In fact, are pets or production and food consumption, the most important pathways. The clinical manifestations of the disease can be from mild to severe symptoms, including watery diarrhea, sometimes with blood, abdominal pain, fever, headache and nausea. Sometimes the infection can get complications after the diarrhea stops and starts a progressive paralysis that can result in respiratory failure and severe neurological dysfunction and may even lead to death. The occurrence of campylobacteriosi's outbreaks should be mandatory to the authorities of surveillance, to provide an investigation of common sources and transmission control through preventive measures , thereby minimizing the potential risks to human health.

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“…animais, bem como, determinar o impacto da sua presença em alimentos e no meio-ambiente (AQUINO et al, 2002;SCARCELLI et al, 2005;ANDRADE et al, 2007;AQUINO et al, 2010;BIASI et al, 2011;QUETZ et al, 2012;ROSSI et al, 2012;MELO et al, 2013). v.73, n.5, p.1683-1685, 2007. …”

Section: N T R O D U ç ã O | 42unclassified

Caracterização molecular de linhagens de Campylobacter jejuni de origens diversas isoladas no Brasil

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Campylobacter jejuni strains isolated from chicken meat harbour several virulence factors and represent a potential risk to humans

Cited by 38 publications

References 24 publications

Macrolide resistance mechanisms and virulence factors in erythromycin-resistant <i>Campylobacter</i> species isolated from chicken and swine feces and carcasses

Macrolide resistance mechanisms and virulence factors in erythromycin-resistant <i>Campylobacter</i> species isolated from chicken and swine feces and carcasses

Campylobacteriosis: an emerging zoonosis, underdiagnosed and underreported by public health agencies in Brazil

Caracterização molecular de linhagens de Campylobacter jejuni de origens diversas isoladas no Brasil

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