Campylobacter spp. contamination of chicken carcasses during processing in relation to flock colonisation

Allen, V. M.; Bull, S. A.; Corry, Janet E L; Domingue, G.; Jørgensen, Frieda; Frost, J. A.; Whyte, R.; González, Ana; Elviss, Nicola; Humphrey, Tom J.

doi:10.1016/j.ijfoodmicro.2006.07.011

Cited by 149 publications

(125 citation statements)

References 36 publications

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“…The Campylobacter-infected flocks may be a source of these bacteria for the corresponding carcasses, although the presence of the same bacterial species in the paired samples (flock -carcass) might also be due to cross-contamination during a slaughter process. These kind of transmission was also confirmed by other authors (Allen et al 2007, Ellerbroek et al 2010. Furthermore, the identification of other Campylobacter species on carcasses than those in the original flocks may also suggest a different contamination sources and routes.…”

Section: Discussionsupporting

confidence: 80%

“…However. contamination of broiler carcasses during processing can occur at various points such as scalding, plucking, defeathering, evisceration or chilling operations (Allen et al 2007, Reich et al 2008.…”

Section: Discussionmentioning

confidence: 99%

“…Poultry are asymptomatic carriers of Campylobacter and the infected flocks cannot be identified by clinical symptoms in birds (Berry et al 1988, Newell et al 2001, Newell and Fearnley 2003, Wieczorek and Osek 2005, Adkin et al 2006, Bull et al 2006, Allen et al 2007, Wirz et al 2010, Hue et al 2011, Ridley et al 2011, Habib et al 2012. The contamination of broiler meat with C. jejuni or C. coli from the chicken intestine may occur during slaughter processing through several routes, such as the air, water, previously slaughtered Campylobacter-positive flocks, equipment used in abattoirs, insects or slaughterhouse personnel (Rivoal et al 1999, Newell et al 2001, Nauta et al 2007, Normand et al 2008, Reich et al 2008, Wirz et al 2010, Hue et al 2011, Habib et al 2012).…”

mentioning

confidence: 99%

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Poultry flocks as a source of Campylobacter contamination of broiler carcasses

Wieczorek¹,

Osek²

2015

Polish Journal of Veterinary Sciences

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Campylobacter infection is the leading foodborne bacterial gastroenteritis worldwide and the bacteria are frequently isolated from the intestines of chickens. The broiler meat contamination with C. jejuni or C. coli may occur during slaughter processing. The aim of the study was to investigate the prevalence of Campylobacter in poultry flocks and the corresponding broiler carcasses in 15 districts (voivodeships) all over Poland. A total of 128 samples from broiler flocks and the corresponding carcasses were collected between February 2011 and April 2013. The Campylobacter isolation and species identification were performed according to ISO 10272-1 standard and with PCR. It was found that 112 flock (96.5%) were contaminated with campylobacters, either C. jejuni (77 samples; 68.7%) or C. coli (35 flocks; 31.3%). Analysis of the corresponding chicken carcasses tested after chilling revealed that 77 out of 128 (60.2%) samples were positive for Campylobacter, either C. jejuni (58; 75.3%) or C. coli (19; 24.7%). Most of the carcasses were contaminated with the same Campylobacter species as identified in the corresponding flock before slaughter. As tested by PCR, out of the 77 crops with C. jejuni 58 were positive for the same bacterial species. On the other hand, out of the remaining 35 flocks infected with C. coli, only 19 corresponding carcass samples were contaminated with C. coli. In three cases in the slaughtered flocks C. jejuni was identified but in the same carcasses C. coli was found. The opposite findings (flock positive for C. coli but the corresponding carcasses contaminated with C. jejuni) were seen in six voivodeships. It was also observed that several carcass samples were negative for C. jejuni and C. coli although the original flocks were Campylobacter-positive before slaughter (total 36 of the 77 samples; 46.7%). On the other hand, some carcasses were contaminated with Campylobacter although the flocks were negative for these bacteria (9 samples; 11.7%) which may also be due to internal contamination during slaughter of broilers.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Poultry flocks as a source of Campylobacter contamination of broiler carcasses

Wieczorek¹,

Osek²

2015

Polish Journal of Veterinary Sciences

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“…Characterization of such isolates with MLST has enabled direct comparison of isolates from a wide variety of samples, and has been consistent with that undertaken with earlier, less precise, typing methods. This indicates that flocks themselves are the source of the majority of strains contaminating the end product, although cross contamination in the abattoir is also significant (Allen et al, 2007;Colles et al, 2010;Wirz et al, 2010). The Campylobacter isolates recovered after slaughter from one free-range broiler crop were more similar to those recovered from retail chicken meat than to isolates obtained from the live flock before slaughter, implying that production processes have an important impact on the differential survival of Campylobacter genotypes .…”

Section: Campylobacter In Animalsmentioning

confidence: 98%

Campylobacter sequence typing databases: applications and future prospects

Colles

Maiden

2012

Microbiology

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“…(Slader et al 2002;Hansson et al 2005;Allen et al 2008;Peyrat et al 2008). Because these crates circulate between unrelated flocks, they may present a contamination risk for uninfected birds Newell et al 2001;Miwa et al 2003;Posch et al 2006;Van Worth et al 2006;Allen et al 2007). The cleaning of equipment involved in poultry processing, including transportation crates, is ineffective , and better decontamination of these crates may be expected to reduce the numbers of pathogens circulating between flocks.…”

Section: Introductionmentioning

confidence: 99%

Campylobacter genotypes from poultry transportation crates indicate a source of contamination and transmission.

Hastings¹,

Colles²,

McCarthy³

et al. 2010

Journal of Applied Microbiology

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Aims-Crates used to transport live poultry can be contaminated with Campylobacter, despite periodic sanitization, and are potential vectors for transmission between flocks. We investigated the microbial contamination of standard and silver ion containing crates in normal use and the genetic structure of associated Campylobacter populations.Methods and Results-Bacteria from crates were enumerated by appropriate culture techniques, and multilocus sequence typing (MLST) was used to determine the genetic structure of Campylobacters isolated from standard and silver ion containing crates. Compared to standard crates, counts of bacteria, including Campylobacter, were consistently lower on silver ion containing crates throughout the decontamination process. In total, 16 different sequence types were identified from 89 Campylobacter jejuni isolates from crates. These were attributed to putative source population (chicken, cattle, sheep, the environment, wild bird) using the population genetic model, STRUCTURE. Most (89%) were attributed to chicken, with 22% attribution to live chicken and 78% to retail poultry meat. MLST revealed a progressive shift in allele frequencies through the crate decontamination process. Campylobacter on crates survived for at least 3 h after sanitization, a period of time equivalent to the journey from the processing plant to the majority of farms in the catchment, showing the potential for involvement of crates in transmission.Conclusions-Inclusion of a silver ion biocide in poultry transportation crates to levels demonstrating acceptable antibacterial activity in vitro reduces the level of bacterial contamination during normal crate use compared to standard crates. Molecular analysis of Campylobacter isolates indicated a change in genetic structure of the population with respect to the poultryprocessing plant sanitization practice.Significance and Impact of the Study-The application of a sustainable antimicrobial to components of poultry processing may contribute to reducing the levels of Campylobacter circulating in poultry.

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Campylobacter spp. contamination of chicken carcasses during processing in relation to flock colonisation

Cited by 149 publications

References 36 publications

Poultry flocks as a source of Campylobacter contamination of broiler carcasses

Poultry flocks as a source of Campylobacter contamination of broiler carcasses

Campylobacter sequence typing databases: applications and future prospects

Campylobacter genotypes from poultry transportation crates indicate a source of contamination and transmission.

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