Can aminopeptidase N determined in the meconium be a candidate for biomarker of fetal intrauterine environment?

Lisowska-Myjak, Barbara; Wilczyńska, Paulina; Bartoszewicz, Zbigniew; Jakimiuk, Artur; Skarżyńska, Ewa

doi:10.1016/j.yexmp.2020.104446

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…The presence of components accumulating in meconium from week 12 confirms that they are in direct contact with the fetus, while their properties and concentrations may provide information about their actual biological role(s) in the intrauterine environment [13]. The possible sources of these meconium components are bile, amniotic fluid swallowed by the fetus or fetal urine [13]. The presence of transferrin and ferritin in meconium has been confirmed by earlier proteomic studies [12].…”

Section: Introductionmentioning

confidence: 73%

“…Meconium is proposed as a readily available and non-invasively obtainable biological material formed during the fetal period which remains in the intestines of the developing fetus to be passed only after birth [12,13]. The presence of components accumulating in meconium from week 12 confirms that they are in direct contact with the fetus, while their properties and concentrations may provide information about their actual biological role(s) in the intrauterine environment [13]. The possible sources of these meconium components are bile, amniotic fluid swallowed by the fetus or fetal urine [13].…”

Section: Introductionmentioning

confidence: 99%

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Meconium Transferrin and Ferritin as Markers of Homeostasis in the Developing Fetus

Skarżyńska,

Mularczyk,

Issat

et al. 2023

IJMS

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The molecular mechanisms regulating homeostasis in the developing fetus have not been satisfactorily elucidated. Meconium contains substances accumulated in the fetal intestines. Measurements of transferrin and ferritin concentrations in meconium and assessment of transferrin–ferritin relationships could enhance knowledge about specific processes of the intrauterine period involving the two proteins and their effects on the development and growth of the fetus. Transferrin and ferritin concentrations were measured by ELISA in the homogenates of first meconium portions from 125 neonates. Higher birth weight was associated with lower ferritin concentrations in meconium (r = −0.22, p = 0.015). In neonates with a birth weight of more than 3750 g, there was a positive correlation between transferrin and ferritin concentrations (r = 0.51, p = 0.003). With meconium transferrin concentrations above 43.52 µg/g, a negative correlation between transferrin and ferritin was established (r = −0.37, p = 0.036), while with transferrin concentrations below 43.52 µg/g, the correlations between the birth weight and the meconium transferrin and ferritin concentrations were negative (r = −0.61, p < 0.001 and r = −0.43, p = 0.017, respectively). Measurements of transferrin and ferritin in meconium specimens create a new use for these common biomarkers to improve our understanding of the effects of homeostasis in utero on the fetal development and growth. Establishing reference ranges of meconium transferrin and ferritin concentrations and their association with the clinical parameters during pregnancy could aid in the assessment of the impact of intrauterine life on the health status of the neonate and its adaptation to extrauterine life.

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Section: Introductionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Meconium Transferrin and Ferritin as Markers of Homeostasis in the Developing Fetus

Skarżyńska,

Mularczyk,

Issat

et al. 2023

IJMS

Self Cite

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Meconium Proteins Involved in Iron Metabolism

Skarżyńska,

Jakimiuk,

Issat

et al. 2024

IJMS

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The lack of specific biological materials and biomarkers limits our knowledge of the mechanisms underlying intrauterine regulation of iron supply to the fetus. Determining the meconium content of proteins commonly used in the laboratory to assess the transport, storage, and distribution of iron in the body may elucidate their roles in fetal development. Ferritin, transferrin, haptoglobin, ceruloplasmin, lactoferrin, myeloperoxidase (MPO), neutrophil gelatinase-associated lipocalin (NGAL), and calprotectin were determined by ELISA in meconium samples obtained from 122 neonates. There were strong correlations between the meconium concentrations of haptoglobin, transferrin, and NGAL (p < 0.05). Meconium concentrations of ferritin were several-fold higher than the concentrations of the other proteins, with the exception of calprotectin whose concentration was approximately three-fold higher than that of ferritin. Meconium ceruloplasmin concentration significantly correlated with the concentrations of MPO, NGAL, lactoferrin, and calprotectin. Correlations between the meconium concentrations of haptoglobin, transferrin, and NGAL may reflect their collaborative involvement in the storage and transport of iron in the intrauterine environment in line with their recognized biological properties. High meconium concentrations of ferritin may provide information about the demand for iron and its utilization by the fetus. The associations between ceruloplasmin and neutrophil proteins may indicate the involvement of ceruloplasmin in the regulation of neutrophil activity in the intrauterine environment.

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Can aminopeptidase N determined in the meconium be a candidate for biomarker of fetal intrauterine environment?

Cited by 2 publications

References 21 publications

Meconium Transferrin and Ferritin as Markers of Homeostasis in the Developing Fetus

Meconium Transferrin and Ferritin as Markers of Homeostasis in the Developing Fetus

Meconium Proteins Involved in Iron Metabolism

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