Can bacterial type <scp>III</scp> effectors mediate pathogen–plant–microbiota ternary interactions?

Liu, Xiaoli; Cai, Jun; Li, Xiaoxu; Yu, Feng; Wu, Dousheng

doi:10.1111/pce.14185

Cited by 5 publications

(2 citation statements)

References 76 publications

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“…Only a small number of genetic determinants in bacterial commensals influencing microbe-microbe and microbe-host interactions during plant microbiota establishment have thus far been identified (Getzke et al, 2023;Hemmerle et al, 2022;Liu et al, 2022). Bacterial DNA barcoding was applied to investigate antagonistic interactions of Pseudomonads leaf strains co-occurring on Arabidopsis (Shalev et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous tracking of near-isogenic bacterial strains in syntheticArabidopsismicrobiota by chromosomally-integrated barcodes

Ordon

Thouin

Nakano

et al. 2023

Preprint

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DNA amplicon-based microbiota profiling currently relies on polymorphisms in microbial marker genes to estimate species diversity and abundance. However, this technique cannot resolve genetic differences among microbial individuals of the same species. We report here the development of modular bacterial tags (MoBacTags) encoding DNA barcodes. These tags facilitate tracking of near-isogenic bacterial commensals in synthetic communities (SynComs), which allow assessment of the contributions of individual bacterial genes to root microbiota establishment inArabidopsis thaliana. Chromosomally-integrated DNA barcodes are co-amplified with endogenous marker genes of the community by integrating corresponding primer binding sites into the barcode. We generated MoBacTag-labeled strains of wild-typePseudomonas capeferrumWCS358 and of pqqF and cyoB mutants with known defects in gluconic acid-mediated host immunosuppression and validated reduced root colonization of both mutants in a 15-member synthetic microbiota. We detected a reduced SynCom load on roots in the presence of the WCS358:pqqF mutant, but not WCS358:cyoB, revealing distinctpqqFandcyoBactivities in a community context. Using MoBacTags, we also show that WCS358 pqqF mutant-specific colonization and community establishment is nottrans-complemented by wild-type WCS358. Given that gluconic acid production inP. capeferrumis indirectly abolished in the pqqF mutant by disruption of pyrroloquinoline quinone (PQQ) biosynthesis, we propose that drastic changes in the root-associated community result from depletion of the cofactor PQQ, which might serve as a common good during root microbiota establishment. Our proof-of-principle experiments illustrate how MoBacTags can be applied to assess scaling of individual bacterial genetic determinants in the plant microbiota.

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Section: Discussionmentioning

confidence: 99%

Simultaneous tracking of near-isogenic bacterial strains in syntheticArabidopsismicrobiota by chromosomally-integrated barcodes

Ordon

Thouin

Nakano

et al. 2023

Preprint

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“…Both these systems are also involved in other roles such as symbiotic interactions including mutualism, commensalism, and pathogenesis [4,[31][32][33][34][35][36]. As recently hypothesized, pathogenic bacteria may also use the T3SS to indirectly target microbiota populations [37]. On the other hand, additional roles of the T6SS have come to light in recent years, including anti-fungal activities and extracellular metal uptake [38][39][40][41].…”

Section: The General Functions and Biology Of Bacterial Secretion Sys...mentioning

confidence: 99%

Bacterial secretion system functions: evidence of interactions and downstream implications

2023

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Unprecedented insights into the biology and functions of bacteria have been and continue to be gained through studying bacterial secretion systems in isolation. This method, however, results in our understanding of the systems being primarily based on the idea that they operate independently, ignoring the subtleties of downstream interconnections. Gram-negative bacteria are naturally able to adapt to and navigate their frequently varied and dynamic surroundings, mostly because of the covert connections between secretion systems. Therefore, to comprehend some of the linked downstream repercussions for organisms that follow this discourse, it is vital to have mechanistic insights into how the intersecretion system functions in bacterial rivalry, virulence, and survival, among other things. To that purpose, this paper discusses a few key instances of molecular antagonistic and interdependent relationships between bacterial secretion systems and their produced functional products.

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The type IX secretion system: Insights into its function and connection to glycosylation in Cytophaga hutchinsonii

Song

Zhuang²,

Tan³

et al. 2022

Engineering Microbiology

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Can bacterial type III effectors mediate pathogen–plant–microbiota ternary interactions?

Cited by 5 publications

References 76 publications

Simultaneous tracking of near-isogenic bacterial strains in syntheticArabidopsismicrobiota by chromosomally-integrated barcodes

Simultaneous tracking of near-isogenic bacterial strains in syntheticArabidopsismicrobiota by chromosomally-integrated barcodes

Bacterial secretion system functions: evidence of interactions and downstream implications

The type IX secretion system: Insights into its function and connection to glycosylation in Cytophaga hutchinsonii

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