Monitoring soil health indicators (SHI) will help ensure that corn (Zea mays L.) stover harvest is sustainable. This study examines SHI changes after 5 yr of growing continuous corn with either chisel plow or no-tillage practices and harvesting 0, ~35, or ~60% of the stover. Two no-tillage treatments with a cereal rye (Secale cereale L.) cover crop and stover harvest rates of ~35 or ~60% were evaluated. All eight treatments were replicated four times in a randomized complete block design at an 11-ha site in Boone county, IA. Soil samples were collected following grain and stover harvest from 0-to 5-and 5-to 15-cm depth increments. Particulate organic matter c (POM-c) decreased when stover was removed or the soil was chisel plowed. no-till with 0% stover removal had 10 mg g -1 POM-c in the 0-to 5-cm soil layer, which was 1.9-fold higher than in other treatments. Potentially mineralizable n (PMn) was greater under cover crop treatments. Average PMn values were 56.9 and 45.5 µg g -1 PMn for no-till with cereal rye at 0-to 5-and 5-to 15-cm depths, respectively, compared with 17.5 and -3.7 µg g -1 PMn for the same no-till treatments without cereal rye. Other soil properties did not respond to increasing levels of stover removal. At this location and at the studied removal rates, 5 yr of harvesting corn stover did not decrease soil health, but POM-c data suggest that changes may be occurring. Long-term monitoring should continue to assess corn stover harvest sustainability.Abbreviations: C0, chisel plow with no stover removal; MBC, microbial biomass C; NT0, no-till with no stover removal; NT35, no-till with moderate stover removal; NT60, no-till with high stover removal; NTR35, no-till with moderate stover removal and rye cover crop; NTR60, no-till with high stover removal and rye cover crop; PMN, potentially mineralizable N; POM-C, particulate organic matter C; POM-N, particulate organic matter N; SHI, soil health indicators; SMAF, Soil Management Assessment Framework; SOC, soil organic C. C orn residues protect soils from the erosive forces of water and wind, maintain soil organic C (SOC) stocks, cycle essential plant nutrients, replenish the C that creates and sustains aggregation, and provide food and energy for the microbial community Ruis et al., 2017;Wilhelm et al., 2007Wilhelm et al., , 2010. Removing an excessive amount of corn stover, defined as the harvested portion to distinguish it from residues left in the field, can result in soil degradation (Blanco-Canqui et al., 2014;Halvorson and Stewart, 2015;Moebius-Clune et al., 2008). However, without stover harvest, producers can encounter residue management problems with subsequent crops and therefore often increase their tillage intensity to reduce surface residues (Al-Kaisi et al., 2015;Sindelar et al., 2013;Swan et al., 1987).A review of stover harvest literature suggests that 40% removal by mass (i.e., 60% remaining in the field) was an upper limit for maintaining SOC and preventing erosion (Ruis et al., 2017;Wilhelm et al., 2010). Johnson et al. (2014) co...