Can established cultured papilloma cells harbor bovine papillomavirus?

Campos, Stella Andrade Rodrigues; Trindade, Carlos S.; Ferraz, O. P.; Giovanni, D.N.S.; Lima, Angélica Alves; Caetano, H.V.A.; Carvalho, Rogério Falleiros; Birgel, Eduardo Harry; Dagli, M.L.; Mori, Ênio; Brandão, P. E.; Richtzenhain, L. J.; Beçak, Willy; Stocco, Rino

doi:10.4238/vol7-4gmr498

Cited by 15 publications

(18 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The identification of several genotypes of papilloma virus (BPV-1, BPV-2, and BPV-13) indicated mixed infections, which were widespread in animals with cutane- ous warts in diverse portions of their body. These results are in agreement with those of Campos et al [43], who also found that cattle, as well as buffalo, had mixed infections of BPV-1, BPV-2, and BPV-13 [44]. We did not find any significant differences in lesions, either grossly or histologically, between samples in relation to specific genotypes, meaning that there may need to be specific studies in the future focusing on these parameters.…”

Section: Discussionsupporting

confidence: 82%

Molecular Epidemiology of Bovine Papillomatosis and Identification of Three Genotypes in Central Iraq

et al. 2017

View full text Add to dashboard Cite

Objective: This study aims to provide a molecular and epidemiological characterization of bovine papillomavirus (BPV) infections in Iraq. Methods: The present study focuses on identifying BPV based on clinical and epidemiological manifestations, histopathological examinations, and polymerase chain reactions (PCR). Samples were collected from 163 animals suffering from cutaneous bovine papillomatosis, including 129 females (79.14%) with an age range of 16–40 months and 34 males (20.85%) with an age range of 17–29 months. Results: The incidence rate was significantly higher in females than in males. The most commonly affected sites were the teats and neck, though warts were found in other areas of the body. Histological sections were diagnosed as fibropapilloma. PCR results showed that 80.13% of the extracted papilloma DNA samples corresponded to the BPV-1 genotype. Furthermore, 7.94% of the samples showed a mixed infection of BPV-1 and BPV-13. While, 40.63% of the extracted DNA blood samples showed 2 DNA fragments corresponding to both genotypes BPV-1 and BPV-2. Conclusions: This study confirmed the presence of BPV-1, BPV-2, and BPV-13, which belong to the Deltapapillomavirus genera, for the first time in the DNA of Iraqi cattle. Understanding BPV diversity and epidemiology is of critical importance for starting prevention strategies.

show abstract

Section: Discussionsupporting

confidence: 82%

Molecular Epidemiology of Bovine Papillomatosis and Identification of Three Genotypes in Central Iraq

et al. 2017

View full text Add to dashboard Cite

show abstract

“…, 2015), placenta (Roperto et al,. 2012) and primary cell cultures (Campos et al , 2008; Campos et al , 2013). Similar results have also been described for HPV (Chiou et al , 2003; Foresta et al , 2013; Pessoa, 2014).…”

Section: Discussionmentioning

confidence: 99%

Papillomaviruses: a systematic review

et al. 2017

View full text Add to dashboard Cite

In the last decades, a group of viruses has received great attention due to its relationship with cancer development and its wide distribution throughout the vertebrates: the papillomaviruses. In this article, we aim to review some of the most relevant reports concerning the use of bovines as an experimental model for studies related to papillomaviruses. Moreover, the obtained data contributes to the development of strategies against the clinical consequences of bovine papillomaviruses (BPV) that have led to drastic hazards to the herds. To overcome the problem, the vaccines that we have been developing involve recombinant DNA technology, aiming at prophylactic and therapeutic procedures. It is important to point out that these strategies can be used as models for innovative procedures against HPV, as this virus is the main causal agent of cervical cancer, the second most fatal cancer in women.

show abstract

“…These data suggest that the in vitro systems are additional and useful model to study the biology of BPV, as discussed by Campos et al [18]. The analysis of cell cycle showed a high percentage of BPVinfected cell in S and/or G2-M phases (figure 2), demonstrating the hyperproliferative action of BPV in vitro.…”

Section: Discussionmentioning

confidence: 52%

Primary Cultures Derived From Bovine Papillomavirus-Infected Lesions As Model To Study Metabolic Deregulation

Stocco¹

2016

Journal of Cancer Research and Therapeutic Oncology

View full text Add to dashboard Cite

Bovine papillomavirus (BPV) is the etiological agent of bovine papillomatosis, disease characterized by the presence of multiple papillomas that can regress or to progress to malignances. Due to the pathological similarities with the human papillomavirus (HPV), BPV is considered a prototype to study the papillomavirus-associated oncogenic process. Although it is clear that both BPV and HPV can interact with host chromatin, the interaction of these viruses with cell metabolism remains understudied due to the little attention given to primary cultures derived from papillomavirus-infected lesions. Thus, this study analyzed the energy metabolism, including the mitochondrial membrane potential (ΔΨm) and Reactive Oxygen Species (ROS) of cells derived from cutaneous papilloma, fibropapilloma and Esophageal Carcinoma (EC) as model to evaluate the cell metabolism. These cells were cultivated until sixth passage and subjected to BPV DNA sequences identification by PCR using specific primers to BPV-1, 2 and 4. PCR results showed the presence and maintenance of at least one BPV type along the six passages analyzed. Cells derived from normal skin, without BPV DNA sequences were used as control. Results of energy metabolism showed the loss of ΔΨm in fibropapilloma and EC cells, suggesting a metabolic switch compatible to the activation of aerobic glycolysis. Cutaneous papilloma and normal skin cells showed the maintenance of ΔΨm. Paradoxically, cutaneous papilloma and fibropapilloma presented high levels of ROS production, while the EC cells reduced the ROS levels, reinforcing the activation of glycolytic metabolism. Our results suggest that the metabolic switch is mediated by BPV E6 oncoprotein, since the addition of this oncoprotein in normal cells promoted the oxidative stress. The oxidative stress showed able to activate the STAT3 nuclear factor in papilloma and fibropapilloma cells, contributing to metabolic deregulation. These data suggest that primary cultures are useful model to study the interaction between BPV and cell metabolism.

show abstract

Can established cultured papilloma cells harbor bovine papillomavirus?

Cited by 15 publications

References 11 publications

Molecular Epidemiology of Bovine Papillomatosis and Identification of Three Genotypes in Central Iraq

Molecular Epidemiology of Bovine Papillomatosis and Identification of Three Genotypes in Central Iraq

Papillomaviruses: a systematic review

Primary Cultures Derived From Bovine Papillomavirus-Infected Lesions As Model To Study Metabolic Deregulation

Contact Info

Product

Resources

About