Can group- and serovar-specific proteins be detected in Ureaplasma urealyticum?

Sa, Horowitz; Duffy, Lynn B.; Garrett, B K; Stephens, John E.; Jk, Davis; Gh, Cassell

doi:10.1097/00006454-198611010-00029

Cited by 22 publications

(26 citation statements)

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“…40% (413/1,061) of the clinical isolates, while 6% (67/1,061) were not typeable and 4% (42/1,061) showed conflicting species and serovar results. It has been observed since the earliest Ureaplasma typing studies that many clinical isolates contain multiple serovars (10,17,29,32,50). Although cross-reactive typing reagents and mixed cultures were generally accepted as plausible explanations, it has never been completely clear whether certain strains can contain multiple serovar specificities and how this may occur.…”

Section: Discussionmentioning

confidence: 99%

“…Although several studies reported that U. urealyticum is more pathogenic than U. parvum (1,13,26,30,34,36), conflicting results have been found by others (6,18), so it is possible that differential pathogenicity might exist at the serovar level rather than at the species level. Inconsistent results implicating specific serovars (or serovar groups) with various clinical conditions (10,12,29,33,46,50,58) coupled with frequent detection of Ureaplasma isolates comprised of more than one serovar have also been reported (10,17,29,32,50). Differing results among investigations could be related to inadequate or imprecise typing methods.…”

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confidence: 99%

“…Differing results among investigations could be related to inadequate or imprecise typing methods. The possibility that individual Ureaplasma isolates may express multiple serovar specificities has been suggested, but never investigated in a systematic manner due to the technical difficulty of separating all of the 14 serovars (17,50).…”

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Extensive Horizontal Gene Transfer in Ureaplasmas from Humans Questions the Utility of Serotyping for Diagnostic Purposes

et al. 2011

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Ureaplasma parvum and Ureaplasma urealyticum are sexually transmitted, opportunistic pathogens of the human urogenital tract. There are 14 known serovars distributed between the two species. For decades, it has been postulated based upon limited data that virulence is related to serotype specificity. The results were often inconclusive due to the small sample size and extensive cross-reactivity between certain serovars. We developed real-time quantitative PCRs that allow reliable differentiation of the two species and type strains of each of the 14 serovars. To investigate species and serovar distributions, we typed 1,061 clinical isolates of human ureaplasmas from diverse patient populations. There was only a tenuous association between individual Ureaplasma serovars and certain patient populations. This may in part be explained by the fact that almost 40% of the isolates were genetic mosaics, apparently arising from the recombination of multiple serovars. This explains the extensive cross-reactivity based upon serotyping and the lack of consistent association of given serotypes with disease.

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Section: Discussionmentioning

confidence: 99%

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Extensive Horizontal Gene Transfer in Ureaplasmas from Humans Questions the Utility of Serotyping for Diagnostic Purposes

et al. 2011

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“…It is disconcerting that up to 96% of isolates are typeable with antisera only to serovars 1 to 10 (84) and that up to 94% are typeable with antisera to serovars 1 to 8 (56), while other studies using antisera to all 14 serovars found that serovars 11 through 14 can occur in 20 to 50% of patients (104). These results may be explained by the tendency of clinical isolates to express multiple specificities (54,84,126,157). This is in part because of cross-reactivity alone but is also because of actual common expression of epitopes (54,126,157).…”

Section: Neonatal Sepsismentioning

confidence: 99%

“…These results may be explained by the tendency of clinical isolates to express multiple specificities (54,84,126,157). This is in part because of cross-reactivity alone but is also because of actual common expression of epitopes (54,126,157). Admittedly, part of the problem may be lack of standardization of reagents and methods between laboratories.…”

Section: Neonatal Sepsismentioning

confidence: 99%

Ureaplasma urealyticum intrauterine infection: role in prematurity and disease in newborns

et al. 1993

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Ureaplasma urealyticum, a common commensal of the urogenital tract of sexually mature humans, is gaining recognition as an important opportunistic pathogen during pregnancy. While its etiologic significance in many aspects of adverse pregnancy remains controversial, recent evidence indicates that U. urealyticum in the absence of other organisms is a cause of chorioamnionitis. Furthermore, ureaplasmal infection of the chorioamnion is significantly associated with premature spontaneous labor and delivery. In at least some cases, it appears to be causal. Present evidence indicates that U. urealyticum is a cause of septicemia, meningitis, and pneumonia in newborn infants, particularly those born prematurely. There is strong but not definitive evidence that ureaplasmal infection of the lower respiratory tract can lead to development of chronic lung disease in very low-birth-weight infants. Although risk factors for colonization of the lower genitourinary tract have been identified, little information is available concerning risk factors for intrauterine infection and host immune responses to invasive infection. Recent establishment of animal models of respiratory and central nervous system diseases should provide an opportunity to evaluate risk factors, pathogenic mechanisms, and operative immune mechanisms. However, the most critical need is additional information concerning indications for diagnosis and treatment as well as efficacy of treatment.

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Ureaplasma

Robertson¹,

Taylor‐Robinson²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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U.re.a.plas'ma. N.L. fem. n. urea urea; Gr. neut. n. plasma anything formed or moulded, image, figure; N.L. neut. n. Ureaplasma urea form. Tenericutes / Mollicutes / Mycoplasmatales / Mycoplasmataceae / Ureaplasma Coccoid cells about 500 nm in diameter ; may appear as coccobacillary forms in exponential growth phase; filaments are rare. Nonmotile. Facultative anaerobes. Form exceptionally small colonies on solid media that are described either as tiny ( T ) “ fried‐egg ” colonies or as “ cauliflower head ” colonies having a lobed periphery. Unusual pH required for growth ( about 6 . 0–6 . 5 ). Optimal incubation temperature for examined species is 35–37°C. Chemo‐organotrophic. Like Mycoplasma , species of Ureaplasma lack oxygen‐dependent, NADH oxidase activity . Unlike Mycoplasma , species of Ureaplasma lack hexokinase or arginine deiminase activities but have a unique and obligate requirement for urea and produce potent ureases that hydrolyze urea to CO 2 and NH 3 for energy generation and growth. Genome sizes range from 760 to 1170 kbp (PFGE). Commensals or opportunistic pathogens in vertebrate hosts, primarily birds and mammals (mainly primates, ungulates, and carnivores). DNA G + C content ( mol %): 25–32 (Bd, T m ). Type species : Ureaplasma urealyticum Shepard, Lunceford, Ford, Purcell, Taylor‐Robinson, Razin and Black 1974, 167 emend. Robertson, Stemke, Davis, Harasawa, Thirkell, Kong, Shepard and Ford 2002, 593.

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Can group- and serovar-specific proteins be detected in Ureaplasma urealyticum?

Cited by 22 publications

References 0 publications

Extensive Horizontal Gene Transfer in Ureaplasmas from Humans Questions the Utility of Serotyping for Diagnostic Purposes

Extensive Horizontal Gene Transfer in Ureaplasmas from Humans Questions the Utility of Serotyping for Diagnostic Purposes

Ureaplasma urealyticum intrauterine infection: role in prematurity and disease in newborns

Ureaplasma

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