Background: The current global situation of COVID-19 epidemic is serious. Routine preoperative methods to assess airway such as the interincisor distance, Mallampati classification, and the upper lip bite test have a certain risk of upper respiratory tract exposure and virus spread. The condyle-tragus maximal distance can be used to assess the airway, and the assessment method does not require the patient to expose the upper respiratory tract, but its value in predicting difficult laryngoscopy compared to other indicators (Mallampati classification, interincisor distance, and upper lip bite test) remains unknown. The purpose of this study was to observe the value of condyle-tragus maximal distance to predict difficult laryngoscopy, and provide a new idea for preoperative airway assessment during epidemic. Methods: We enrolled adult patients who underwent general anesthesia and tracheal intubation. The interincisor distance, Mallampati test result, upper lip bite test result, and the condyle-tragus maximal distance of each patient were evaluated prior to the initiation of anesthesia. The primary outcome was difficult laryngoscopy defined as the Cormack-Lehane Level > grade 2. Results: A total of 304 patients were successfully included in the study ,39 patients were identified as difficult laryngoscopy. The correlation between the condyle-tragus maximal distance and Cormack-Lehane Level was the highest (Spearman correlation coefficient, -0.317; P<0.001), and the area under the ROC curve was the highest (AUC:0.699,P<0.01). The condyle-tragus maximal distance <1 finger width was the most consistent with difficult laryngoscopy (κ=0.485;99% CI,0.286-0.612) and its OR value was 10.09(95%CI: 4.19-24.28), sensitivity was 0.469(95%CI: 0.325-0.617), specificity was 0.929(95%CI: 0.877-0.964), positive predictive value was 0.676 (95%CI: 0.484-0.745), negative predictive value was 0.847(95%CI :0.825-0.865). Conclusion: Compared with the interincisor distance , Mallampati classification and the upper lip bite test, the condyle-tragus maximal distance has higher value in predicting difficult laryngoscopy, which can become a safer airway assessment method during the epidemic of COVID-19. Trial registration: The study was registered on October 21, 2019 in the Chinese Clinical Trial Registry (ChiCTR1900026775).