Methods: Prospective study of 308 low-risk women. Shortened CL was defined as < 25 mm (< 5th centile), while raised EMG activity was defined as presence of > 20 action potentials in 20 minutes of assessment (> 95th centile). Outcome measures were diagnostic accuracy of both tests for prediction of PTD and early PTD (< 34 weeks).Results: The incidence of PTD was 23/308 (7.4%) while the incidence of early was 9/308 (2.9%). Shortened CL and raised EMG activity are significantly related to PTD (prevalence-weighted likelihood ratio (pwLR) 1.9 (95%CI 1.0-3.5) versus 9.5 (95%CI 2.5-35.7), but not to early PTD (pwLR 0.4 (95%CI 0.2-0.8) versus 0.6 (95%CI 0.3-1.7).Significant predictive value for early PTD was found only if shortened CL was concomitant with raised EMG activity (pwLR 4 (95% CI 1.3-14.3).Conclusion: Shortened CL and raised EMG activity in the second trimester have significant accuracy regarding the prediction of PTD in a low-risk population. However, in order to be useful as a predictor for early PTD, both tests must be positive.