Colposcopy is considered a stressful and painful procedure. High anxiety levels stem from a lack of knowledge about the procedure and fear of pain. One of the best ways to relieve anxiety is to provide women with knowledge to improve awareness, cooperation during procedures and compliance. Video assisted-teaching is considered as an effective method to provide accurate practical knowledge, decrease anxiety levels and reduce the pain experience among women undergoing colposcopy. Aim of the study: is to evaluate the effect of video assisted-teaching on level of knowledge, anxiety and pain among women undergoing colposcopy. Research design: A quasi-experimental research design was utilized. Setting: The study was conducted at the colposcopy outpatient clinics at Obstetrics and Gynecology outpatient department at El Kaser Aliniy University Hospital, Cairo University, Egypt. Sample: A purposive sample of 70 women was recruited for the study. Tools: Five tools were used for data collection 1) Women structured interviewing questionnaire; 2) Beck Anxiety Inventory (BAI) scale; 3) Numerical Pain Rating Scale (NPRS); 4) Knowledge Assessment Questionnaires and 5) Video assisted-teaching and booklet. Results: The mean age of the women was 38.90±11.21years old.