Can parasite-derived microRNAs differentiate active and inactive cystic echinococcosis patients?

Örsten, Serra; Baysal, İpek; Yabanoğlu-Çiftçi, Samiye; Çiftçi, Türkmen; Ünal, Emre; Akıncı, Devrim; Akyön, Yakut; Akhan, Okan

doi:10.1007/s00436-021-07382-7

Cited by 10 publications

(3 citation statements)

References 36 publications

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“…15 According to a recent report, three highly expressed parasite-derived miRNAs (egr-let-7-5p, egr-miR-71a-5p, and egr-miR-9-5p) were found to be upregulated in the serum of Cystic Echinococcosis (CE) patients when compared to the active CE patients. ROC analysis showed that the egr-let-7-5p can not only discriminate between healthy and CE patients (AUC = 0.9170), but it also distinguish between active and inactive CE patients (AUC 23 The present study demonstrated the let-7-5p-based RCA-assisted CRISPR/ Cas9 method effectively distinguished between healthy controls and metacestode-infected animals. The diagnostic sensitivity and specificity for rabbit cysticercosis were 100% and 97.67%, respectively, with an AUC of 0.9992.…”

Section: Discussionmentioning

confidence: 54%

“…17 Previous studies have revealed that cestodes share the same miRNA let-7 (let-7-5p), 18 which has been shown to be one of the highly expressed miRNAs in cestodes. [19][20][21] In addition, the expression level of let-7-5p was significantly upregulated in the serum and liver of metacestodes-infected animals and humans, [22][23][24] suggesting its potential as a target molecule for diagnosing metacestodiasis.…”

Section: Introductionmentioning

confidence: 99%

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Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9

Wang,

Pu,

Liu

et al. 2024

The FASEB Journal

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Metacestodiasis is an infectious disease caused by the larval stage of cestode parasites. This disease poses a serious health hazard to wildlife, livestock, and humans, and it incurs substantial economic losses by impacting the safety of the livestock industry, the quality of meat production, and public health security. Unfortunately, there is currently no available molecular diagnostic method capable of distinguishing cysticercus‐ and Echinococcus‐derived microRNAs (miRNAs) from other helminthes and hosts in the plasma of metacestode‐infected animals. This study aims to develop a specific, sensitive, and cost‐efficient molecular diagnostic method for cysticercosis and echinococcosis, particularly for early detection. The study developed a rolling circular amplification (RCA)‐assisted CRISPR/Cas9 detection method based on parasite‐derived miRNA let‐7‐5p. Using a series of dilutions of the let‐7 standard, the limit of detection (LOD) of the qPCR, RCA, and RCA‐assisted CRISPR/Cas9 methods was compared. The specificity of qPCR and CRISPR/Cas9 was evaluated using four artificially synthesized let‐7 standards from different species. A total of 151 plasma samples were used to evaluate the diagnostic performance. Additionally, the study also assessed the correlation between plasma levels of let‐7‐5p, the number of Taenia pisiformis cysticerci, and the weight of Echinococcus multilocularis cysts. The results demonstrated that the RCA‐assisted CRISPR/Cas9 assay could significantly distinguish let‐7 from cestodes and other species, achieving a LOD of 10 aM; the diagnostic sensitivity and specificity for rabbit cysticercosis and mouse E. multilocularis were 100% and 97.67%, and 100% and 100%, respectively. Notably, let‐7‐5p gradually increased in the plasma of T. pisiformis‐infected rabbits from 15 days post infection (dpi), peaked at 60 dpi, and persisted until 120 dpi. In E. multilocularis‐infected mice, let‐7‐5p gradually increased from 15 dpi and persisted until 90 dpi. Furthermore, the expression of let‐7‐5p positively correlated with the number of cysticerci and cyst weight. These results indicated that the let‐7‐5p‐based RCA‐assisted CRISPR/Cas9 assay is a sensitive and specific detection method that can be used as a universal diagnostic method for metacestodiasis, particularly for early diagnosis (15 dpi).

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Section: Discussionmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9

Wang,

Pu,

Liu

et al. 2024

The FASEB Journal

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“…A recent study evaluated the potential of three parasite mi-RNAs of E. granulosus (egr-le-7-5p, egrmiR-71a-5p and egr-miR-9-5p), which are expressed in both the protoscolex and cyst wall, not only as biomarkers of EC but also to distinguish between active and inactive CE. Based on the results, the authors concluded that egr-let-7-5p and egr-miR-9-5p showed potential biomarkers able for the differential diagnosis of active and inactive CE [26 ▪▪ ].…”

Section: Diagnosismentioning

confidence: 99%

Cystic echinococcosis of the bone

Monge-Maillo,

Lopez-Velez

2023

Current Opinion in Infectious Diseases

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Purpose of review Cystic echinococcosis (CE) has a wide world distribution causing important morbidity. Osseous involvement is present in less than 4% of the CE cases. Its diagnosis and therapeutic management is full of challenges and low grade of evidence. Recent findings The study summarizes literature evidence on the management of osseous CE with particular emphasis on new data regarding diagnosis and treatment. Summary Clinical presentation of osseous CE depends on the skeletal area affected. Diagnosis is mostly based on radiological findings and serology. Recent advances with qPCR on osseous tissue samples seem to be a good option for diagnosis confirmation. Complete resection of the cystic lesion is the only curative option, but it is usually not possible performing palliative surgery and prolonged albendazole intake in most cases. Radiotherapy could be an option, but experience to date is only based on clinical cases.

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Serum Level of egr-miR-2a-3p as a Potential Diagnostic Biomarker for Cystic Echinococcosis

et al. 2022

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Can parasite-derived microRNAs differentiate active and inactive cystic echinococcosis patients?

Cited by 10 publications

References 36 publications

Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9

Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9

Cystic echinococcosis of the bone

Serum Level of egr-miR-2a-3p as a Potential Diagnostic Biomarker for Cystic Echinococcosis

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