Can Peripheral Blood Mononuclear Cells be Used as a Proxy for Mitochondrial Dysfunction in Vital Organs During Hemorrhagic Shock and Resuscitation?

Karamercan, Mehmet Akif; Weiss, Scott L.; Villarroel, Jose Paul Perales; Guan, Yihui; Werlin, Evan; Figueredo, Ronald; Becker, Lance B.; Sims, Carrie A.

doi:10.1097/shk.0b013e3182a5175b

Cited by 3 publications

(4 citation statements)

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“…permeabilized RBCs vs. permeabilized muscle; our attempts to permeabilize RBCs were not successful), we showed that mitochondrial parameters measured in intact RBCs moderately correlated to those measured in permeabilized pectoral muscle fibres (see Table 3 for details). Interestingly, similar findings have been found in humans between blood mononuclear cells and organs such as kidney and heart (Karamercan et al 2013). Altogether, it indicates that mitochondrial parameters are to some extent correlated among tissues, including blood.…”

Section: O R R E L a T I O N S B E T W E E N M I T O C H O N D R I supporting

confidence: 82%

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How to measure mitochondrial function in birds using red blood cells: a case study in the king penguin and perspectives in ecology and evolution

et al. 2017

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Summary 221. Mitochondria are the powerhouse of animal cells. They produce through oxidative 23 phosphorylation more than 90% of the cellular energy (ATP) required for organism's 24 growth, reproduction and maintenance. Hence, information on mitochondrial 25 function is expected to bring important insights in animal ecology and evolution. 26Unfortunately, the invasiveness of the procedures required to measure 27 mitochondrial function (e.g. sampling of liver or muscles) has limited its study in wild 28 vertebrate populations so far. Here, we capitalize on the fact that bird red blood cells 29 (RBCs) possess functional mitochondria to describe a minimally--invasive approach to 30 study mitochondrial function using blood samples. 31 2. In the king penguin, we present a protocol using a high--resolution respirometry 32 system and specific agonists and antagonists enabling the assessment of 33 mitochondrial function in RBCs. We evaluated the inter--assay repeatability of our 34 measures of mitochondrial function, and tested the influence of sample storage and 35 bird handling time on these measures. We also compared measures of mitochondrial 36 function in RBCs and in the pectoral muscle obtained from the same individuals. opens new opportunities to study mitochondrial function in free--living animals and 48 could bring knowledge gains in ecology and evolution. Fish, amphibians and reptiles 49 also possess mitochondria in their RBCs, and the approach presented here could also 50 be applicable to these taxa. 51 52

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Section: O R R E L a T I O N S B E T W E E N M I T O C H O N D R I supporting

confidence: 82%

“…Interestingly, similar findings have been found in humans between blood mononuclear cells and organs such as kidney and heart (Karamercan et al . ). Altogether, it indicates that mitochondrial parameters are to some extent correlated among tissues, including blood.…”

Section: Discussionmentioning

confidence: 97%

How to measure mitochondrial function in birds using red blood cells: a case study in the king penguin and perspectives in ecology and evolution

et al. 2017

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“…Some studies have shown that mitochondrial function measured in blood cells is correlated to some extent to mitochondrial function in other tissues such as kidneys, heart, skeletal muscles and brain (e.g. [22,[37][38][39], while some other studies did not find any significant relationship (e.g. [40]).…”

Section: High-resolution Respirometry Analysis Of Mitochondrial Function In Intact Blood Cellsmentioning

confidence: 98%

Experimental demonstration of prenatal programming of mitochondrial aerobic metabolism lasting until adulthood

Stier

Monaghan²,

Metcalfe³

2021

Preprint

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It is increasingly being postulated that among-individual variation in mitochondrial function underlies variation in individual performance (e.g. growth rate) and state of health. Environmental conditions experienced before birth have been suggested to programme postnatal mitochondrial biology, but the hypothesis that early-life conditions induce consistent and long-lasting differences in mitochondrial function among individuals remains mostly untested. We tested this hypothesis in an avian model by experimentally manipulating prenatal conditions (incubation temperature and stability), then measuring mitochondrial aerobic metabolism in blood cells from the same individuals during postnatal growth and at adulthood. Mitochondrial aerobic metabolism changed markedly across life stages, and part of these age-related changes were influenced by the prenatal experimental conditions. A high incubation temperature induced a consistent and long-lasting increase in mitochondrial aerobic metabolism, while unstable incubation conditions had a delayed effect, with an increased metabolism only being observed at adulthood. While we detected significant within-individual consistency in mitochondrial aerobic metabolism across life-stages, the prenatal temperature regime only accounted for a relatively small proportion (<20%) of the consistent among-individual differences we observed. Our results demonstrate that prenatal conditions can program consistent and long-lasting differences in mitochondrial function, which could potentially underly among-individual variation in performance and health state.

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“…As such, there is considerable interest in more direct measures of mitochondrial dysfunction. Peripheral blood mononuclear cells (PBMCs) have often been used for this purpose (10)(11)(12)(13)(14), as they provide an accessible source of mitochondria, have demonstrated moderate correlation with mitochondrial changes in other organ systems (15), and are directly involved in the immuno-inflammatory response characteristic of sepsis and other illnesses (3,16,17). However, PBMCs are a heterogenous mixture of lymphocyte and monocyte subtypes with variable metabolic profiles.…”

Section: Introductionmentioning

confidence: 99%

Influence of Immune Cell Subtypes on Mitochondrial Measurements in Peripheral Blood Mononuclear Cells From Children with Sepsis

Weiss

Henrickson

Lindell

et al. 2021

Shock

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Introduction:Peripheral blood mononuclear cells (PBMCs) are commonly used to compare mitochondrial function in patients with versus without sepsis, but how these measurements in this mixed cell population vary by composition of immune cell subtypes is not known, especially in children. We determined the effect of changing immune cell composition on PBMC mitochondrial respiration and content in children with and without sepsis.Methods:PBMC mitochondrial respiration and citrate synthase (CS) activity, a marker of mitochondrial content, were measured in 167 children with sepsis at three timepoints (day 1–2, 3–5, and 8–14) and once in 19 nonseptic controls. The proportion of lymphocytes and monocytes and T, B, and NK cells was measured using flow cytometry. More specific CD4+ and CD8+ T cell subsets were measured from 13 sepsis patients and 6 controls. Spearman's correlation and simple and mixed effects linear regression were used to determine the association of PBMC mitochondrial measures with proportion of immune cell subtypes.Results:PBMC mitochondrial respiration and CS activity were correlated with proportion of monocytes, lymphocytes, T B, and NK cells in controls, but not in sepsis patients. PBMC mitochondrial respiration was correlated with CD4+ and CD8+ T cell subsets in both groups. After controlling for differences in immune cell composition between groups using linear regression models, PBMC respiration and CS activity remained lower in sepsis patients than controls.Conclusions:Mitochondrial measurements from PBMCs varied with changes in immune cell composition in children with and without sepsis. However, differences in PBMC mitochondrial measurements between sepsis patients and controls were at least partially attributable to the effects of sepsis rather than solely an epiphenomena of variable immune cell composition.

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Can Peripheral Blood Mononuclear Cells be Used as a Proxy for Mitochondrial Dysfunction in Vital Organs During Hemorrhagic Shock and Resuscitation?

Cited by 3 publications

References 5 publications

How to measure mitochondrial function in birds using red blood cells: a case study in the king penguin and perspectives in ecology and evolution

How to measure mitochondrial function in birds using red blood cells: a case study in the king penguin and perspectives in ecology and evolution

Experimental demonstration of prenatal programming of mitochondrial aerobic metabolism lasting until adulthood

Influence of Immune Cell Subtypes on Mitochondrial Measurements in Peripheral Blood Mononuclear Cells From Children with Sepsis

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