2022
DOI: 10.1134/s0003683822060023
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Can Recombinant Tree Shrew (Tupaia belangeri chinensis) Chymosin Coagulate Cow (Bos taurus) Milk?

Abstract: Genetically engineered chymosin from the tree shrew (Tupaia belangeri chinensis) has been obtained and partially characterized for the first time. The target enzyme was produced in Escherichia coli, strain BL21(DE3). It was shown that tree shrew recombinant chymosin coagulates cow milk (Bos taurus). The total and specific milk-clotting activity of the obtained enzyme was 0.7–5.3 IMCU/mL and 8.8–16.6 IMCU/mg. The nonspecific proteolytic activity of tree shrew recombinant chymosin in relation to total bovine cas… Show more

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Cited by 3 publications
(1 citation statement)
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“…From 2001 to the present, the following rChns have been obtained and characterized: sheep (Ovis aries) [13], goat (Capra hircus) [14][15][16], buffalo (Bubalus arnee bubalis) [17,18], yak (Bos grunniens) [19,20], alpaca (Vicugna pacos) [21], Altai maral (Cervus elaphus sibiricus) [22][23][24][25], rabbit (Oryctolagus cuniculus) [26], white whale (Delphinapterus leucas) [27], two-humped camel (Camelus bactrianus) [28,29], and tupaya (Tupaia belangeri chinensis) [30].…”
Section: Introductionmentioning
confidence: 99%
“…From 2001 to the present, the following rChns have been obtained and characterized: sheep (Ovis aries) [13], goat (Capra hircus) [14][15][16], buffalo (Bubalus arnee bubalis) [17,18], yak (Bos grunniens) [19,20], alpaca (Vicugna pacos) [21], Altai maral (Cervus elaphus sibiricus) [22][23][24][25], rabbit (Oryctolagus cuniculus) [26], white whale (Delphinapterus leucas) [27], two-humped camel (Camelus bactrianus) [28,29], and tupaya (Tupaia belangeri chinensis) [30].…”
Section: Introductionmentioning
confidence: 99%