The purpose of this study was to examine the impact of listening to classical music on functional (upper and lower body strength, functional mobility and aerobic endurance) and cognitive (attentional capacities and working memory (WM)) performances in women aged between 50 and 60 years old. A total of 24 middle-aged women were enrolled to participate in this study. Their functional and cognitive performances were assessed under two-auditory conditions (no-music vs. with music conditions) using the Timed Up and Go (TUG) test for functional mobility, the Arm Curl test and 30 s Chair Stand Tests for the upper and lower body strength, respectively, and the 2 min Step test for aerobic endurance. To assess the attentional capacities and the WM, a simple reaction time (SRT) test and Corsi Block-Tapping Task were used, respectively. As a result, we found that listening to music significantly decreased the scores of the TUG test (p < 0.001) and capacities (p < 0.05), and increased the 2 min Step test values (p < 0.001) compared to the no-music condition. However, no significant changes were found for the upper and lower body strength and WM. We conclude that listening to classical music, i.e., Mozart’s Symphony, is effective in improving functional mobility, aerobic endurance and attentional capacities in middle-aged women. However, these gains were absent for muscle strength and WM, suggesting that the positive effects of music on functional and cognitive performances were dependent on a specific task.