Can Solid Matrix Priming with GA 3 Break Seed Dormancy in Eastern Gamagrass?

Abstract

Development of methods for breaking seed dormancy in eastern gamagrass (Tripsacum dactyloides, L.) could increase its use. Solid matrix priming, the controlled hydration of seed in a system of solid carrier and water, has been used with some success to enhance germination in warm-season grasses. gibberellic acid (ga3), a known promoter of eastern gamagrass germination, can be added to solid matrix priming systems. In this study, systems were evaluated for conditioning eastern gamagrass seeds using the solid carriers Agro-Lig, MicroCel E, and Vermiculite #5. GA3 was added in 0.01 M concentration solutions to systems with water potentials of −0.4 and −0.6 MPa in Agro-Lig and −0.2 and −0.4 in MicroCel E and Vermiculite #5 and compared with systems with deionized water. Priming seed with GA3 increased germination to 18% compared with 13% without GA3. MicroCel E and Vermiculite #5 were suitable materials for controlled hydration of eastern gamagrass seed. Germination was only 11% in Agro-Lig compared with 16%-19% for MicroCel E and Vermiculite #5. Priming with GA3 does not appear to be as successful at breaking seed dormancy as cold, wet stratification. AbstractDevelopment of methods for breaking seed dormancy in eastern gamagrass (Tripsacum dactyloides, L.) could increase its use. Solid matrix priming, the controlled hydration of seed in a system of solid carrier and water, has been used with some success to enhance germination in warm-season grasses. Gibberellic acid (GA 3 ), a known promoter of eastern gamagrass germination, can be added to solid matrix priming systems. In this study, systems were evaluated for conditioning eastern gamagrass seeds using the solid carriers Agro-Lig, MicroCel E, and Vermiculite #5. GA 3 was added in 0.01 M concentration solutions to systems with water potentials of À0.4 and À0.6 MPa in Agro-Lig and À0.2 and À0.4 in MicroCel E and Vermiculite #5 and compared with systems with deionized water. Priming seed with GA 3 increased germination to 18% compared with 13% without GA 3. MicroCel E and Vermiculite #5 were suitable materials for controlled hydration of eastern gamagrass seed. Germination was only 11% in Agro-Lig compared with 16%-19% for MicroCel E and Vermiculite #5. Priming with GA 3 does not appear to be as successful at breaking seed dormancy as cold, wet stratification. ResumenEl desarrollo de métodos para romper la dormancia de las semillas del "Eastern gamagrass" (Tripsacum dactyloides, L.) pudiera incrementar su uso. La matriz soelida de preparació n de la superficie de la semilla, la hidratació n controlada de la semilla en un sistema en un sistema de conductor só lido y agua, ha sido usado con algo de éxito para mejorar la germinació n del zacates de estació n caliente. El á cido gibérelico (GA3), un conocido promotor de la germinació n del "Eastern gamagrass", puede ser agregado en los sistemas de matriz só lida de preparació n de la superficie de la semilla. En este estudio, los sistemas fueron evaluados para acondicionar semillas de "Eastern gamagrass" usand...

The germination niches of grassland species targeted for restoration: effects of seed pre-treatments

“…(2) osmotic seed priming was carried out by soaking seeds in a solution of polyethylene glycol (PEG)-6000 with a nominal osmotic potential of 2 1.0 MPa, for which the weight ratios of PEG-6000 and distilled water were calculated with the computer program SPMM (Michel and Radcliffe, 1995); and (3) osmotic priming was also applied in combination with a seed pre-soaking in gibberellic acid (GA 3 ). In agricultural and horticultural species, the latter can further increase benefits associated with priming (Finch-Savage, 1991; Rogis et al, 2004). To this end, we dissolved GA 3 in the PEG solution at a concentration of 1000 mg l 21 .…”

“…We would thus not generally recommend such a combination, especially as GA 3 , at higher concentrations, can induce development of morphologically abnormal seedlings with reduced fitness (e.g. Rogis et al, 2004). We tested only a limited range of pre-treatments, and variation of factors, such as pre-treatment duration, PEG solution concentration, temperature during the pre-treatment phase or use of other priming methods, may increase pre-treatment efficiency.…”

“…In agricultural and horticultural species, the latter can further increase benefits associated with priming (Finch-Savage, 1991;Rogis et al, 2004). To this end, we dissolved GA 3 in the PEG solution at a concentration of 1000 mg l 21 .…”

Application of solid matrix priming to ameliorate salinity stress in mung bean (Vigna radiata)

“…In our experiment, GA 3 showed the best results among all hormonal treatments in all attributes. Previous works revealed that SMP by GA 3 can break seed dormancy and enhance seed germination in eastern gama grass (Tripsacum dactyloides L.) (Rogis et al, 2004). Khan et al (2004) also reported similar trends showing alleviative effect of GA 3 over salt stress on seed germination.…”