Can the location of the isolation laboratory affect the generation of myxomycete data using moist chambers? An experiment in the Neotropics

Alvarado, Carlos; Rollins, Adam W.; Gutiérrez, Kenneth Matarrita; Camacho, Pedro Rojas

doi:10.5943/cream/11/1/6

Cited by 1 publication

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“…This approach has had a very positive impact on the documentation of myxomycetes worldwide (e.g., Härkönen 1977), mainly because it represents a robust secondary method of recording such organisms, after field collecting. Over time, however, researchers have documented some limitations of the method (Alexopoulos 1969, Schnittler et al 2002, and recently, based on Neotropical research (Rojas et al 2021a, c) few experimental shortcomings have been addressed as well. One of the main issues is that the technique calls for its application in "standard" laboratory conditions which are never controlled in real life situations and are most likely different every time the method is used, even in the same laboratory.…”

Section: Introductionmentioning

confidence: 99%

Results of myxomycete experiments could be affected by temperature in “standard” laboratory conditions

Rojas¹,

Valverde²

2023

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Moist chamber cultures are valuable for myxomycete research and can be used for the analysis of ecological patterns with implications in ecosystem conservation programs. However, to make comparisons between datasets valid, the method should be redesigned considering potential biases affecting the generation of results. In the present study, both the effect of the general climate of the laboratory and two microclimatic variables within the moist chamber were studied in relation with the obtained data. Of all the recorded variables, temperature was observed to affect the results, both at the level of the general laboratory climate and in relation with the microclimate of the moist chamber. Increments in laboratory and moist chamber temperature increased the probability, three or fourfold, associated with a higher number of records or species within a group of equivalent samples. Such probabilistic differences are significant enough to suggest that using the moist chamber technique in “standard” laboratory conditions is not enough for cross-dataset comparisons that increase the potential of myxomycete data for applications outside of the biological sciences.

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Section: Introductionmentioning

confidence: 99%

Results of myxomycete experiments could be affected by temperature in “standard” laboratory conditions

Rojas¹,

Valverde²

2023

View full text Add to dashboard Cite

show abstract

Can the location of the isolation laboratory affect the generation of myxomycete data using moist chambers? An experiment in the Neotropics

Cited by 1 publication

References 0 publications

Results of myxomycete experiments could be affected by temperature in “standard” laboratory conditions

Results of myxomycete experiments could be affected by temperature in “standard” laboratory conditions

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