Can tissue engineering concepts advance tumor biology research?

Hutmacher, Dietmar W.; Loessner, Daniela; Rizzi, Simone C.; Kaplan, David L.; Mooney, David J.; Clements, Judith A.

doi:10.1016/j.tibtech.2009.12.001

Cited by 216 publications

(186 citation statements)

References 75 publications

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“…For instance, before effective vascularization, the tumor growth appears to be closely reproduced in 3D culture systems, and the proliferation of tumor cells is typically slower and hence more indicative of physiological growth than that in monolayer cultures. To bridge the gap between 2D studies and in vivo animal models, the 3D culture of cancer cells in vitro has been presented in several recent studies [2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

3D polylactide-based scaffolds for studying human hepatocarcinoma processesin vitro

Scaffaro

Rigogliuso

et al. 2012

Science and Technology of Advanced Materials

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We evaluated the combination of leaching techniques and melt blending of polymers and particles for the preparation of highly interconnected three-dimensional polymeric porous scaffolds for in vitro studies of human hepatocarcinoma processes. More specifically, sodium chloride and poly(ethylene glycol) (PEG) were used as water-soluble porogens to form porous and solvent-free poly(L,D-lactide) (PLA)-based scaffolds. Several characterization techniques, including porosimetry, image analysis and thermogravimetry, were combined to improve the reliability of measurements and mapping of the size, distribution and microarchitecture of pores. We also investigated the effect of processing, in PLA-based blends, on the simultaneous bulk/surface modifications and pore architectures in the scaffolds, and assessed the effects on human hepatocarcinoma viability and cell adhesion. The influence of PEG molecular weight on the scaffold morphology and cell viability and adhesion were also investigated. Morphological studies indicated that it was possible to obtain scaffolds with well-interconnected pores of assorted sizes. The analysis confirmed that SK-Hep1 cells adhered well to the polymeric support and emitted surface protrusions necessary to grow and differentiate three-dimensional systems. PEGs with higher molecular weight showed the best results in terms of cell adhesion and viability.

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Section: Introductionmentioning

confidence: 99%

3D polylactide-based scaffolds for studying human hepatocarcinoma processesin vitro

Scaffaro

Rigogliuso

et al. 2012

Science and Technology of Advanced Materials

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“…The crosstalk between cells and their microenvironment can be investigated if these processes are broken-down and systematically analysed using a versatile technology platform that incorporates tissue-specific extracellular matrix (ECM) elements [1]. Tissue engineering has produced advanced tools that integrate cells into scaffolds and matrices that mimic specific features and the 3-dimensionality (3D) of their natural niche [2]. To design a tissue-specific technology platform, cells need to differentiate and secrete ECM that represents key characteristics of their surrounding microenvironment.…”

Section: Introductionmentioning

confidence: 99%

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

et al. 2017

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds, Acta Biomaterialia (2016), doi: http://dx.doi.org/10. 1016/j.actbio.2016.12.040 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. models that mimic the endosteal microenvironment enable researchers to discover the causes and improve treatments for blood and immune-related diseases. The aim of this study was to establish a physiologically relevant in vitro model using 3D printed scaffolds to assess the contribution of human cells to the formation of a construct that mimics human endosteum. Melt electrospun written scaffolds were used to compare the suitability of primary human osteoblasts (hOBs) and placenta-derived mesenchymal stem cells (plMSCs) in ( when compared to HSCs maintained using tissue culture plastic. This 3D testing platform represents an endosteal bone-like platform and warrants future investigation for the maintenance and expansion of human HSCs.

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“…Although 2-dimensional cell culture models have formed the mainstay of in vitro approaches for cancer research, it has long been recognized that cell behavior is fundamentally altered in monolayer culture systems 18,36,37 . This has led to the development of engineered microenvironments that mimic the complexity of 3-dimensional living tissues, including matrix-and scaffold-based models composed of natural materials such as collagen, or synthetic polymers seeded with specific cell types to create tissue-like microenvironments [36][37][38][39][40][41] . Engineered approaches have also included the use of bioreactor platforms to control and study the hormonal milieu of the microenvironment 18,19,[41][42][43] .…”

Section: Introductionmentioning

confidence: 99%

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

Templeton

Bachmann

Alluri

et al. 2015

JoVE

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Bone is the most common site of breast cancer metastasis. Although it is widely accepted that the microenvironment influences cancer cell behavior, little is known about breast cancer cell properties and behaviors within the native microenvironment of human bone tissue.We have developed approaches to track, quantify and modulate human breast cancer cells within the microenvironment of cultured human bone tissue fragments isolated from discarded femoral heads following total hip replacement surgeries. Using breast cancer cells engineered for luciferase and enhanced green fluorescent protein (EGFP) expression, we are able to reproducibly quantitate migration and proliferation patterns using bioluminescence imaging (BLI), track cell interactions within the bone fragments using fluorescence microscopy, and evaluate breast cells after colonization with flow cytometry. The key advantages of this model include: 1) a native, architecturally intact tissue microenvironment that includes relevant human cell types, and 2) direct access to the microenvironment, which facilitates rapid quantitative and qualitative monitoring and perturbation of breast and bone cell properties, behaviors and interactions. A primary limitation, at present, is the finite viability of the tissue fragments, which confines the window of study to short-term culture. Applications of the model system include studying the basic biology of breast cancer and other bone-seeking malignancies within the metastatic niche, and developing therapeutic strategies to effectively target breast cancer cells in bone tissues. Video LinkThe video component of this article can be found at

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Can tissue engineering concepts advance tumor biology research?

Cited by 216 publications

References 75 publications

3D polylactide-based scaffolds for studying human hepatocarcinoma processesin vitro

3D polylactide-based scaffolds for studying human hepatocarcinoma processesin vitro

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

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