Background: The p rosthetic trochlear design is important in postoperative patellofemoral kinematics and knee function. However, little research has been conducted on the differences in trochlear groove between native and prosthetic knees. We aimed to investigate the differences between Chinese native knees and prosthetic knees of five different femoral component designs using three-dimensional computerized quantification Of the entire trochlear length. Methods: Virtual total knee arthroplasty was performed using three-dimensional models of 42 healthy Chinese knees matched to the femoral components of five different prosthe tic systems by mechanical alignment . T he deepest points of the trochlear groove were marked in multiple cross sections for both the native and prosthetic knees . Taking the lower extremity mechanical axis as reference line, the differences in the mediolateral location of the trochlear groove were analyzed between the native and prosthetic knees. Results: From the proximal to the distal end, the native trochlear groove started from 0° cross section and extended laterally and then medially , with its turning point located at 69° cross section . T he prosthetic trochlear groove showed a similar medial orientation and extended more proximally, but varied in mediolateral location and the length extending to the intercondylar notch . Compared with the proximal portion of the native trochlear groove , the prosthetic knee s extended along a paradoxical orientation and started from a more proximal and lateral position to 3.2 mm in the 0° cross section , with maximal discrepancy. Distally, the prosthetic trochlear groove s were located significantly medial to 2.4 mm in the 69° cross section , with maximal discrepancy. Conclusion: The prosthetic trochlear design varie d among the different types and did not conform to the native knee in terms of shape, orientation, and location, which may cause soft tissue tension imbalance and abnormal patellofemoral biomechanics during knee flexion. This study may provide useful information for creating prosthetic trochlear design s that conform with the native knee anatomy to optimize patellofemoral biomechanics and reduce the risk of patellofemoral complications.